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The map label for this gene is pdhC [H]

Identifier: 17231098

GI number: 17231098

Start: 4356210

End: 4357502

Strand: Direct

Name: pdhC [H]

Synonym: alr3606

Alternate gene names: 17231098

Gene position: 4356210-4357502 (Clockwise)

Preceding gene: 17231097

Following gene: 17231099

Centisome position: 67.92

GC content: 48.18

Gene sequence:

>1293_bases
ATGAGCATTCACGAAATCTTCATGCCGGCGCTGAGTTCCACCATGACCGAAGGCAAAATTGTCTCCTGGGTGAAATCGCC
AGGCGATAAAGTGGAAAAAGGCGAAACAGTGGTGGTTGTCGAGTCAGACAAAGCAGATATGGATGTAGAAACCTTTTATG
AAGGTTTTCTCGCCCACATCATTGTAGAAGCTGGTGATAGCGCACCTGTAGGAGCTGCGATCGCTTACGTAGCGGAAACC
GAAGCCGAAATCGAAGCCGCTAAGTCTTTAGGCAGTTCTGGTGCTGCTGCGGCTACCCCTCCGGCCGCCCCTCAACCAGT
CGCAACTACCGCCGCCGTAGGTGTACCAGCCACATCACAAAATGGCTCTAACCACCGTGAGGGTAGGCTTATAGCTTCAC
CCCGCGCCCGCAAATTGGCAAAAGAACTGAAAGTTGATTTGACAAGTCTCAAAGGTAGTGGCCCCTACGGTCGCATTGTT
GCCGATGACATAGAATCTGCTGTTGGTAAAGTCAAGCAACCAGCCACGACACCATCTGCACCCACACCCACCTTTACCCC
AGCCGCACCCCCAGCACCCCGCACTCCAGCACCAGCGCCTGCACCAATAGCGGCTGCACCTGGGCAAGTAGTACCATTTA
ATACCCTACAAAACGCTGTCATTCGCAACATGGTGGCGAGCCTTGATGTACCCGTGTTCCGTGTGGGTTACACAATCACC
ACCGATGGCTTAGACAAACTTTACAAACAAATTAAATCCAAAGGCGTAACCATGACAGCGCTACTGGCGAAAGCTGTGGC
GGTGACATTACAAAAACATCCTTTATTAAATGCCAGCTATTCAGATCAAGGTCTTGTTTATCATCCTGACATCAATGTTG
CGGTAGCTGTAGCGATGGATGGTGGCGGTTTAATTACACCAGTGTTAAAAAATGCCGACAAGATAGATATCTACTCCCTA
TCACGTACTTGGAAATCTCTAGTAGACAAAGCCAGGAGCAAACAACTACAACCAGACGAATACACTGGTGGTAATTTTAC
CCTGTCAAACTTGGGGATGTTTGGTGTCGATACATTTGATGCCATTTTACCCCCAGGACAAGGTTCAATTTTAGCGATCG
GTGCATCTCGTCCCCAATTGGTAGCTACAGGCGATGGTTTATTTGGTGTCAAACAACAAATGCAGGTAAATATTACATCT
GATCACCGGATTATTTACGGTGCTGATGCTGCCGCATTTTTACAAGATTTAGCCAAGTTGATTGAAACTGATGCTCAATC
TTTGACACTGTAA

Upstream 100 bases:

>100_bases
TTCCCAATTCCCAATTACTCAAAAACCCTCGTACCAAGATACAATTCGATCTGGCAAAAGCTGTTAAATTCCTTAAACCC
ATTGATTCTGAAACCACCCT

Downstream 100 bases:

>100_bases
AGGTGTAAGGGTGTAAGGGTGAACTTTTCTGTATTCTCACCCCTATACCCCATACCCCTATACCCCCAGCCTCGGTTTAT
CATAGATCAGAGCATGATAG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGFLAHIIVEAGDSAPVGAAIAYVAET
EAEIEAAKSLGSSGAAAATPPAAPQPVATTAAVGVPATSQNGSNHREGRLIASPRARKLAKELKVDLTSLKGSGPYGRIV
ADDIESAVGKVKQPATTPSAPTPTFTPAAPPAPRTPAPAPAPIAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTIT
TDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINVAVAVAMDGGGLITPVLKNADKIDIYSL
SRTWKSLVDKARSKQLQPDEYTGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGLFGVKQQMQVNITS
DHRIIYGADAAAFLQDLAKLIETDAQSLTL

Sequences:

>Translated_430_residues
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGFLAHIIVEAGDSAPVGAAIAYVAET
EAEIEAAKSLGSSGAAAATPPAAPQPVATTAAVGVPATSQNGSNHREGRLIASPRARKLAKELKVDLTSLKGSGPYGRIV
ADDIESAVGKVKQPATTPSAPTPTFTPAAPPAPRTPAPAPAPIAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTIT
TDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINVAVAVAMDGGGLITPVLKNADKIDIYSL
SRTWKSLVDKARSKQLQPDEYTGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGLFGVKQQMQVNITS
DHRIIYGADAAAFLQDLAKLIETDAQSLTL
>Mature_429_residues
SIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGFLAHIIVEAGDSAPVGAAIAYVAETE
AEIEAAKSLGSSGAAAATPPAAPQPVATTAAVGVPATSQNGSNHREGRLIASPRARKLAKELKVDLTSLKGSGPYGRIVA
DDIESAVGKVKQPATTPSAPTPTFTPAAPPAPRTPAPAPAPIAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTITT
DGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINVAVAVAMDGGGLITPVLKNADKIDIYSLS
RTWKSLVDKARSKQLQPDEYTGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGLFGVKQQMQVNITSD
HRIIYGADAAAFLQDLAKLIETDAQSLTL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=32.9545454545455, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=31.1659192825112, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=31.2087912087912, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI110671329, Length=413, Percent_Identity=27.1186440677966, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=38.3233532934132, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI19923748, Length=167, Percent_Identity=36.5269461077844, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=26.2790697674419, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1786305, Length=416, Percent_Identity=28.8461538461538, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=29.9776286353468, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=26.1792452830189, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=291, Percent_Identity=29.553264604811, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25146366, Length=165, Percent_Identity=32.1212121212121, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=32.4384787472036, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320352, Length=425, Percent_Identity=25.4117647058824, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321632, Length=188, Percent_Identity=28.7234042553192, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129315, Length=442, Percent_Identity=32.579185520362, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=31.4942528735632, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=26.7441860465116, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645909, Length=166, Percent_Identity=32.5301204819277, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44833; Mature: 44701

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGFLAHI
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
IVEAGDSAPVGAAIAYVAETEAEIEAAKSLGSSGAAAATPPAAPQPVATTAAVGVPATSQ
HEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEECCCCCCC
NGSNHREGRLIASPRARKLAKELKVDLTSLKGSGPYGRIVADDIESAVGKVKQPATTPSA
CCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCC
PTPTFTPAAPPAPRTPAPAPAPIAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEEEE
TDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINVAVAVAMD
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEC
GGGLITPVLKNADKIDIYSLSRTWKSLVDKARSKQLQPDEYTGGNFTLSNLGMFGVDTFD
CCCEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCHHH
AILPPGQGSILAIGASRPQLVATGDGLFGVKQQMQVNITSDHRIIYGADAAAFLQDLAKL
HHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCEEEEECCHHHHHHHHHHH
IETDAQSLTL
HHCCHHHCCC
>Mature Secondary Structure 
SIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGFLAHI
CHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
IVEAGDSAPVGAAIAYVAETEAEIEAAKSLGSSGAAAATPPAAPQPVATTAAVGVPATSQ
HEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEECCCCCCC
NGSNHREGRLIASPRARKLAKELKVDLTSLKGSGPYGRIVADDIESAVGKVKQPATTPSA
CCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCC
PTPTFTPAAPPAPRTPAPAPAPIAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEEEE
TDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINVAVAVAMD
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEC
GGGLITPVLKNADKIDIYSLSRTWKSLVDKARSKQLQPDEYTGGNFTLSNLGMFGVDTFD
CCCEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCHHH
AILPPGQGSILAIGASRPQLVATGDGLFGVKQQMQVNITSDHRIIYGADAAAFLQDLAKL
HHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCEEEEECCHHHHHHHHHHH
IETDAQSLTL
HHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]