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The map label for this gene is anfA [H]

Identifier: 17231050

GI number: 17231050

Start: 4294476

End: 4297001

Strand: Reverse

Name: anfA [H]

Synonym: all3558

Alternate gene names: 17231050

Gene position: 4297001-4294476 (Counterclockwise)

Preceding gene: 17231051

Following gene: 17231049

Centisome position: 67.0

GC content: 43.27

Gene sequence:

>2526_bases
ATGACATCTCCAAACACAGTTATATGGCTACGAGAACGGACAACTTTAGGTATTCTCTCATCGATGGTGTTGGATGCGAT
CGCCCAAACTCTGGAAGAGCAAGTTGTACCAGCCCACAGTACTCTAGTTTCAGAAGCTACTCACCCAGAAGCACTGTACA
TTGTCCAAGATGGTCAACTAGAAAGCGAGACTACTAATCAAAACAGTTCGGCTTTAGCTCGTGGATTTCTACCGGGGGCA
GTCATTAATCTGCAAGAATTACTTTTGGATGAATTAACACCATGTAAAATTGTCACCCTGACAGAATCTCATGTGTGGGT
CATACCTGCGGCTAAATTTCGGGACTTAATTAGCCAATACCCGGAGATTAATCAGGCTTTTTCTCGACAATTAGCCCAAG
AATTAGCCCAGGTGACATCTGCGTTAACCTATGAGCAAGAACGTTCTGTAGCGTTGCGTCCCTATTTAGTCACCAAAGCG
CAACGGGGAATTGTCGGTACGAGTCGCTACGCTGTGCGTCTGCGAGAGCAAATTCGCGTCGCGGCCACTGACCGCAAATC
TGTAGAAATCTTTGGCGAACCTGGCTTAGAAAAAGATAATATCGCCACTCTGATTCACTTCAGTTCCCCCAAACGACGGG
AACCGATTATTAAAATCAATTGTGGGATTTTACAGACAAGCGGTGCAGATTTATTTGGTCGTGCTGGCGGTAAACCTGGA
CTTTTGGAGTGGTTGGGGGAAGGTAGTTTAATTCTCAATAATATCCAAGAATTACCCCCAGAGTTATTACAGCCTGTAGC
ACAGTTACTGCAAACAAATATATATACTCCCGTTTCTCGACCAGGAGAACCAGCTGCTGCACCCCTTACCAGTAAAGCCC
GGATTCTACTTGTGTCGGAAAAAACCGAGTCTAAGATTGAGCGTTGTGTGGGACATATTATTAAAGTACCACCGCTACGG
GTAAGGAAAGCTGATATTAAAGCCCAGGTAGAATATTACACTAGTTTGTATGTGCGATCGCGTGGACTGAAGAAACCCCA
CATAACCCCAGAAGCTTTACGCCGCCTACAATCTTATGATTTCCCCGGTAATCTCAAGGAGTTAAAAAACCTTGTAGAAA
GGGCAATTATCCAAGCAGGGGAACGCAGGGAATTAACAGAAGAAATATTCTGGGCGGCTGAACCTAAGAAAAAACAATTT
CGTGTCAATCTTTTAAATCTTTATCCAGGGTTGCGGCGGTTTTTACGCAGTTCTTGGTGGCCTGACAAGATTAACTATGG
TTTTACTGTGGCGGCGTTTGCATTTATTGTAGGCGTTTTGTTTGTCGGGCCGCAAACACGCGATCGCAATTTTGCTTTAA
ATTTATTTTGGGCTTGGTGGTGGCCTCTCATCCTCTTGAGTTTCCCCTTCTTCGGGCGTGTGTGGTGTGCTGTCTGTCCC
TTCATGATTTACGGGGAAATCACCCAAAAACTATCTCTGTGGCTATTCCCCCGCCAACTCAAGCGCTGGCCGCGAGAACA
AGCAGAAAAATGGGGCGGATGGTTTCTATTTGGTTTATTTACCTTAATTTTCCTGTGGGAAGAACTTTGGCATTTAGAAA
ATACAGCCTATCTTTCCGCTTGTTTACTATTATTAATTACCTCTGGGGCAATGATTTTTTCTGCCCTGTTTGAGCGACGT
TTTTGGTGTCGCTATCTTTGTCCCATTGGGGGAATGAATGGTTTGTTTGCTAAACTTTCCATGACTGAACTAAGAGCGCA
GCAAGGTATCTGTTCTGCTACCTGTAATACCTATCAATGTTATAAAGGCGGCCCCGAAAAAGGCGAAGGGATGGAAACAG
ATGGTTGTCCCCTATATTCTCACCCAGCCCAGTTAGAAGATAACCGAGATTGTGTACTGTGCATGACTTGTCTCAAAGCT
TGTCCCCATCGTTCTGTAGAATTTAACTTGCGTCCCCCTGGAATTGAACTATGGACAACTCATGTACCCCGCAATTATGA
AGTAGCTTTGTTGTTTTTGTTGTTAGGGGGAATATATATCCATCGTTTACAAGAGTTGTTAGATTGGCTGGGATTACAAA
TTGATTTAACTCAATTTTGGCTACATTTAGGGATATCAATTATAGCATTATTAATGCCATTTGCTATTATTTTATCCAGC
TACTGGTTAATTAAAATCTTCAATACTCGCCGTAAACCTCGACGCTTGATTGAACTTGCTTATGGTTATTTGCCATTAGT
TTTAGGGGGAAATTTTGCTCACTATCTACGTTTAGGTTTAGGTGAAAGTGGCAAGATTCTCCCCGTTACTTTTGCCACAT
TTGGTCTGAGTGGTGAGCAATTACCAATATTAGTAGCACACCCAGCCGTGATTGCCTTTTTACAAGGTGCAACCCTCATT
TTTTCCGTGCTGTTGACGATAGTATTGACGCAAAAAATCGCCCGACAACCATTGAGAACCTTAATTTGGCAACACCTCGC
AGCTATTATTTTGGGTATAAGTATGTGGACAATTATTGTTCTTTAG

Upstream 100 bases:

>100_bases
AAGGAACACCAAGATTAGGTAAATCAGATAAACTCATCAGACAGCTATTTTCAGGGATAATTCCTTTTATACAGCAAGCA
GCCTGCACGGAGAACCCAGG

Downstream 100 bases:

>100_bases
GTGTCGTTATTAGGACTTACGCAAACAGGTTATCTGTTGAGACCGGGTGTAAGGGTTTAAGGGTACAGGGGTGTAAGGGT
TTCAAGCATTTATACTCCTA

Product: nitrogen assimilation regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 841; Mature: 840

Protein sequence:

>841_residues
MTSPNTVIWLRERTTLGILSSMVLDAIAQTLEEQVVPAHSTLVSEATHPEALYIVQDGQLESETTNQNSSALARGFLPGA
VINLQELLLDELTPCKIVTLTESHVWVIPAAKFRDLISQYPEINQAFSRQLAQELAQVTSALTYEQERSVALRPYLVTKA
QRGIVGTSRYAVRLREQIRVAATDRKSVEIFGEPGLEKDNIATLIHFSSPKRREPIIKINCGILQTSGADLFGRAGGKPG
LLEWLGEGSLILNNIQELPPELLQPVAQLLQTNIYTPVSRPGEPAAAPLTSKARILLVSEKTESKIERCVGHIIKVPPLR
VRKADIKAQVEYYTSLYVRSRGLKKPHITPEALRRLQSYDFPGNLKELKNLVERAIIQAGERRELTEEIFWAAEPKKKQF
RVNLLNLYPGLRRFLRSSWWPDKINYGFTVAAFAFIVGVLFVGPQTRDRNFALNLFWAWWWPLILLSFPFFGRVWCAVCP
FMIYGEITQKLSLWLFPRQLKRWPREQAEKWGGWFLFGLFTLIFLWEELWHLENTAYLSACLLLLITSGAMIFSALFERR
FWCRYLCPIGGMNGLFAKLSMTELRAQQGICSATCNTYQCYKGGPEKGEGMETDGCPLYSHPAQLEDNRDCVLCMTCLKA
CPHRSVEFNLRPPGIELWTTHVPRNYEVALLFLLLGGIYIHRLQELLDWLGLQIDLTQFWLHLGISIIALLMPFAIILSS
YWLIKIFNTRRKPRRLIELAYGYLPLVLGGNFAHYLRLGLGESGKILPVTFATFGLSGEQLPILVAHPAVIAFLQGATLI
FSVLLTIVLTQKIARQPLRTLIWQHLAAIILGISMWTIIVL

Sequences:

>Translated_841_residues
MTSPNTVIWLRERTTLGILSSMVLDAIAQTLEEQVVPAHSTLVSEATHPEALYIVQDGQLESETTNQNSSALARGFLPGA
VINLQELLLDELTPCKIVTLTESHVWVIPAAKFRDLISQYPEINQAFSRQLAQELAQVTSALTYEQERSVALRPYLVTKA
QRGIVGTSRYAVRLREQIRVAATDRKSVEIFGEPGLEKDNIATLIHFSSPKRREPIIKINCGILQTSGADLFGRAGGKPG
LLEWLGEGSLILNNIQELPPELLQPVAQLLQTNIYTPVSRPGEPAAAPLTSKARILLVSEKTESKIERCVGHIIKVPPLR
VRKADIKAQVEYYTSLYVRSRGLKKPHITPEALRRLQSYDFPGNLKELKNLVERAIIQAGERRELTEEIFWAAEPKKKQF
RVNLLNLYPGLRRFLRSSWWPDKINYGFTVAAFAFIVGVLFVGPQTRDRNFALNLFWAWWWPLILLSFPFFGRVWCAVCP
FMIYGEITQKLSLWLFPRQLKRWPREQAEKWGGWFLFGLFTLIFLWEELWHLENTAYLSACLLLLITSGAMIFSALFERR
FWCRYLCPIGGMNGLFAKLSMTELRAQQGICSATCNTYQCYKGGPEKGEGMETDGCPLYSHPAQLEDNRDCVLCMTCLKA
CPHRSVEFNLRPPGIELWTTHVPRNYEVALLFLLLGGIYIHRLQELLDWLGLQIDLTQFWLHLGISIIALLMPFAIILSS
YWLIKIFNTRRKPRRLIELAYGYLPLVLGGNFAHYLRLGLGESGKILPVTFATFGLSGEQLPILVAHPAVIAFLQGATLI
FSVLLTIVLTQKIARQPLRTLIWQHLAAIILGISMWTIIVL
>Mature_840_residues
TSPNTVIWLRERTTLGILSSMVLDAIAQTLEEQVVPAHSTLVSEATHPEALYIVQDGQLESETTNQNSSALARGFLPGAV
INLQELLLDELTPCKIVTLTESHVWVIPAAKFRDLISQYPEINQAFSRQLAQELAQVTSALTYEQERSVALRPYLVTKAQ
RGIVGTSRYAVRLREQIRVAATDRKSVEIFGEPGLEKDNIATLIHFSSPKRREPIIKINCGILQTSGADLFGRAGGKPGL
LEWLGEGSLILNNIQELPPELLQPVAQLLQTNIYTPVSRPGEPAAAPLTSKARILLVSEKTESKIERCVGHIIKVPPLRV
RKADIKAQVEYYTSLYVRSRGLKKPHITPEALRRLQSYDFPGNLKELKNLVERAIIQAGERRELTEEIFWAAEPKKKQFR
VNLLNLYPGLRRFLRSSWWPDKINYGFTVAAFAFIVGVLFVGPQTRDRNFALNLFWAWWWPLILLSFPFFGRVWCAVCPF
MIYGEITQKLSLWLFPRQLKRWPREQAEKWGGWFLFGLFTLIFLWEELWHLENTAYLSACLLLLITSGAMIFSALFERRF
WCRYLCPIGGMNGLFAKLSMTELRAQQGICSATCNTYQCYKGGPEKGEGMETDGCPLYSHPAQLEDNRDCVLCMTCLKAC
PHRSVEFNLRPPGIELWTTHVPRNYEVALLFLLLGGIYIHRLQELLDWLGLQIDLTQFWLHLGISIIALLMPFAIILSSY
WLIKIFNTRRKPRRLIELAYGYLPLVLGGNFAHYLRLGLGESGKILPVTFATFGLSGEQLPILVAHPAVIAFLQGATLIF
SVLLTIVLTQKIARQPLRTLIWQHLAAIILGISMWTIIVL

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG0348

COG function: function code C; Polyferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=30.2127659574468, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1789233, Length=232, Percent_Identity=30.1724137931034, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082152, Length=261, Percent_Identity=29.1187739463602, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI87081872, Length=246, Percent_Identity=28.4552845528455, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788550, Length=213, Percent_Identity=30.9859154929577, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1789087, Length=282, Percent_Identity=26.9503546099291, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1790437, Length=240, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=25, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788905, Length=235, Percent_Identity=28.936170212766, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786524, Length=286, Percent_Identity=28.3216783216783, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=205, Percent_Identity=25.3658536585366, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 95331; Mature: 95200

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPNTVIWLRERTTLGILSSMVLDAIAQTLEEQVVPAHSTLVSEATHPEALYIVQDGQL
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCC
ESETTNQNSSALARGFLPGAVINLQELLLDELTPCKIVTLTESHVWVIPAAKFRDLISQY
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHHC
PEINQAFSRQLAQELAQVTSALTYEQERSVALRPYLVTKAQRGIVGTSRYAVRLREQIRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHH
AATDRKSVEIFGEPGLEKDNIATLIHFSSPKRREPIIKINCGILQTSGADLFGRAGGKPG
HCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEECCCCCHHHCCCCCCC
LLEWLGEGSLILNNIQELPPELLQPVAQLLQTNIYTPVSRPGEPAAAPLTSKARILLVSE
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
KTESKIERCVGHIIKVPPLRVRKADIKAQVEYYTSLYVRSRGLKKPHITPEALRRLQSYD
CHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
FPGNLKELKNLVERAIIQAGERRELTEEIFWAAEPKKKQFRVNLLNLYPGLRRFLRSSWW
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDKINYGFTVAAFAFIVGVLFVGPQTRDRNFALNLFWAWWWPLILLSFPFFGRVWCAVCP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHH
FMIYGEITQKLSLWLFPRQLKRWPREQAEKWGGWFLFGLFTLIFLWEELWHLENTAYLSA
HHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
CLLLLITSGAMIFSALFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGICSATCNTYQC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCEEE
YKGGPEKGEGMETDGCPLYSHPAQLEDNRDCVLCMTCLKACPHRSVEFNLRPPGIELWTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEE
HVPRNYEVALLFLLLGGIYIHRLQELLDWLGLQIDLTQFWLHLGISIIALLMPFAIILSS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
YWLIKIFNTRRKPRRLIELAYGYLPLVLGGNFAHYLRLGLGESGKILPVTFATFGLSGEQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCCC
LPILVAHPAVIAFLQGATLIFSVLLTIVLTQKIARQPLRTLIWQHLAAIILGISMWTIIV
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
TSPNTVIWLRERTTLGILSSMVLDAIAQTLEEQVVPAHSTLVSEATHPEALYIVQDGQL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCC
ESETTNQNSSALARGFLPGAVINLQELLLDELTPCKIVTLTESHVWVIPAAKFRDLISQY
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHHC
PEINQAFSRQLAQELAQVTSALTYEQERSVALRPYLVTKAQRGIVGTSRYAVRLREQIRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHH
AATDRKSVEIFGEPGLEKDNIATLIHFSSPKRREPIIKINCGILQTSGADLFGRAGGKPG
HCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEECCCCCHHHCCCCCCC
LLEWLGEGSLILNNIQELPPELLQPVAQLLQTNIYTPVSRPGEPAAAPLTSKARILLVSE
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
KTESKIERCVGHIIKVPPLRVRKADIKAQVEYYTSLYVRSRGLKKPHITPEALRRLQSYD
CHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
FPGNLKELKNLVERAIIQAGERRELTEEIFWAAEPKKKQFRVNLLNLYPGLRRFLRSSWW
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDKINYGFTVAAFAFIVGVLFVGPQTRDRNFALNLFWAWWWPLILLSFPFFGRVWCAVCP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHH
FMIYGEITQKLSLWLFPRQLKRWPREQAEKWGGWFLFGLFTLIFLWEELWHLENTAYLSA
HHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
CLLLLITSGAMIFSALFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGICSATCNTYQC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCEEE
YKGGPEKGEGMETDGCPLYSHPAQLEDNRDCVLCMTCLKACPHRSVEFNLRPPGIELWTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEE
HVPRNYEVALLFLLLGGIYIHRLQELLDWLGLQIDLTQFWLHLGISIIALLMPFAIILSS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
YWLIKIFNTRRKPRRLIELAYGYLPLVLGGNFAHYLRLGLGESGKILPVTFATFGLSGEQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCCC
LPILVAHPAVIAFLQGATLIFSVLLTIVLTQKIARQPLRTLIWQHLAAIILGISMWTIIV
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]