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The map label for this gene is hsdS [H]

Identifier: 17230969

GI number: 17230969

Start: 4196580

End: 4197731

Strand: Direct

Name: hsdS [H]

Synonym: alr3477

Alternate gene names: 17230969

Gene position: 4196580-4197731 (Clockwise)

Preceding gene: 17230968

Following gene: 17230970

Centisome position: 65.43

GC content: 39.24

Gene sequence:

>1152_bases
ATGACTGGGACAAAGAATAAAAGAGATTTTTGTTTTACTGCCATAGTAGATGCAACTCACATTGCTGAAAAGAAGCTTTA
CTTAAATCAAAGAGTTGGCTGTATAAGAATAATTAAACCAGTTTATAACCAATTAGTTAACATTTTTTTGAAATCTCCCA
TAATTGTAGATTATCTACTATCATCTGCAACTGGAACTGCAAATCAAGCCAATATAGGAGCCAATACTCTACGGGAACTC
CCATTCCCTCTTCCACCCCTTGCAGAACAAAAACGCATTGTAGAGAAGTGCGATCGCCTGCTTTCCATCTGCGACGAAAT
CGAAAAACGGCACCAGCAAAGGCAAGAGAGCATTGTGAGGATGAATGAGAGTGCGATCGCTCAACTCCTCTCATCCCAAA
ATCCCGACGATTTCCGCCAACACTGGCAGCGCATTTGCAATAATTTTGACTTACTCTACAGCATTCCTGAAACTATTCCT
AAACTGCGTCAGGCGATTTTGCAATTGGCTGTGCAGGGTAAACTAACTAATCAAAGTAGTAAAGAAATCAAAAAAATATC
AGATACTCACAAAGTATCTGATTATGTCAGCATCTTAAATGGTTATGCGTTCAAAAGCACTTGGTTTATCAATGATGGGA
TTCGTCTTTTGCGGAATGCAAACGTTGGTCATGGTGATTTAAGATGGGACGATGTAGCCACAATATCTGAAGAAAGAGCG
CAAGAATTTCAAAGATTCAAGCTAGATATAGATGATATAGTTATATCCCTTGATCGTCCTATCATTTCAACCGGATTAAA
AGTTGCAAGAATTACTAAGAATGACCTTCCATGCCTTCTTTTACAGCGTGTTGGAAAATTCGAGTTTAAAACCGATAAAG
TTATCCCTGATTTTTTCTTCCTGTGGCTACAGTCTCCAATTTTCATAAATGCGATAGATCCAGGCAGAAGTAATGGAGTA
CCACATATTTCATCCAAAAGTATAGAAGCGATTCTCTTCAATCCTCCTTCCAGAGAAGAACAAAAACGGATTGTAGAGAA
GTGCGATCGCCTGATGTCTCTCTGTGATACCCTAGAAGCCAAACTAAAACAAGGGCGAGACAGCAGCGAGAAACTGATGG
AAGTTGCAGCAAAACAGGTTTTAACAGTCTAA

Upstream 100 bases:

>100_bases
TGACGAAATACTTTTGAATCAGAATCCAGTTTTTATAACAGATGAGGTTGCTAGTGAGAGCGATAAATTTTTAATTCATG
AGAATGATATTCTCATAACT

Downstream 100 bases:

>100_bases
TAGCCATTTTCCGTAGGGACGGGTTTAAAAATCTGCTCGATTATTAATGATGATCTTGGTGAACCCGCCCCTACAAACGG
CGTTTAGGCTTACGATAAGG

Product: type I restriction-modification enzyme S subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTGTKNKRDFCFTAIVDATHIAEKKLYLNQRVGCIRIIKPVYNQLVNIFLKSPIIVDYLLSSATGTANQANIGANTLREL
PFPLPPLAEQKRIVEKCDRLLSICDEIEKRHQQRQESIVRMNESAIAQLLSSQNPDDFRQHWQRICNNFDLLYSIPETIP
KLRQAILQLAVQGKLTNQSSKEIKKISDTHKVSDYVSILNGYAFKSTWFINDGIRLLRNANVGHGDLRWDDVATISEERA
QEFQRFKLDIDDIVISLDRPIISTGLKVARITKNDLPCLLLQRVGKFEFKTDKVIPDFFFLWLQSPIFINAIDPGRSNGV
PHISSKSIEAILFNPPSREEQKRIVEKCDRLMSLCDTLEAKLKQGRDSSEKLMEVAAKQVLTV

Sequences:

>Translated_383_residues
MTGTKNKRDFCFTAIVDATHIAEKKLYLNQRVGCIRIIKPVYNQLVNIFLKSPIIVDYLLSSATGTANQANIGANTLREL
PFPLPPLAEQKRIVEKCDRLLSICDEIEKRHQQRQESIVRMNESAIAQLLSSQNPDDFRQHWQRICNNFDLLYSIPETIP
KLRQAILQLAVQGKLTNQSSKEIKKISDTHKVSDYVSILNGYAFKSTWFINDGIRLLRNANVGHGDLRWDDVATISEERA
QEFQRFKLDIDDIVISLDRPIISTGLKVARITKNDLPCLLLQRVGKFEFKTDKVIPDFFFLWLQSPIFINAIDPGRSNGV
PHISSKSIEAILFNPPSREEQKRIVEKCDRLMSLCDTLEAKLKQGRDSSEKLMEVAAKQVLTV
>Mature_382_residues
TGTKNKRDFCFTAIVDATHIAEKKLYLNQRVGCIRIIKPVYNQLVNIFLKSPIIVDYLLSSATGTANQANIGANTLRELP
FPLPPLAEQKRIVEKCDRLLSICDEIEKRHQQRQESIVRMNESAIAQLLSSQNPDDFRQHWQRICNNFDLLYSIPETIPK
LRQAILQLAVQGKLTNQSSKEIKKISDTHKVSDYVSILNGYAFKSTWFINDGIRLLRNANVGHGDLRWDDVATISEERAQ
EFQRFKLDIDDIVISLDRPIISTGLKVARITKNDLPCLLLQRVGKFEFKTDKVIPDFFFLWLQSPIFINAIDPGRSNGVP
HISSKSIEAILFNPPSREEQKRIVEKCDRLMSLCDTLEAKLKQGRDSSEKLMEVAAKQVLTV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 43720; Mature: 43589

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTKNKRDFCFTAIVDATHIAEKKLYLNQRVGCIRIIKPVYNQLVNIFLKSPIIVDYLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SSATGTANQANIGANTLRELPFPLPPLAEQKRIVEKCDRLLSICDEIEKRHQQRQESIVR
HHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNESAIAQLLSSQNPDDFRQHWQRICNNFDLLYSIPETIPKLRQAILQLAVQGKLTNQSS
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KEIKKISDTHKVSDYVSILNGYAFKSTWFINDGIRLLRNANVGHGDLRWDDVATISEERA
HHHHHHHHHHHHHHHHHHHHCCHHCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QEFQRFKLDIDDIVISLDRPIISTGLKVARITKNDLPCLLLQRVGKFEFKTDKVIPDFFF
HHHHHHHCCHHHHHHHCCCHHHHHCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHH
LWLQSPIFINAIDPGRSNGVPHISSKSIEAILFNPPSREEQKRIVEKCDRLMSLCDTLEA
HHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KLKQGRDSSEKLMEVAAKQVLTV
HHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGTKNKRDFCFTAIVDATHIAEKKLYLNQRVGCIRIIKPVYNQLVNIFLKSPIIVDYLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SSATGTANQANIGANTLRELPFPLPPLAEQKRIVEKCDRLLSICDEIEKRHQQRQESIVR
HHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNESAIAQLLSSQNPDDFRQHWQRICNNFDLLYSIPETIPKLRQAILQLAVQGKLTNQSS
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KEIKKISDTHKVSDYVSILNGYAFKSTWFINDGIRLLRNANVGHGDLRWDDVATISEERA
HHHHHHHHHHHHHHHHHHHHCCHHCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QEFQRFKLDIDDIVISLDRPIISTGLKVARITKNDLPCLLLQRVGKFEFKTDKVIPDFFF
HHHHHHHCCHHHHHHHCCCHHHHHCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHH
LWLQSPIFINAIDPGRSNGVPHISSKSIEAILFNPPSREEQKRIVEKCDRLMSLCDTLEA
HHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KLKQGRDSSEKLMEVAAKQVLTV
HHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]