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The map label for this gene is hsdM [H]

Identifier: 17230967

GI number: 17230967

Start: 4194672

End: 4196114

Strand: Direct

Name: hsdM [H]

Synonym: alr3475

Alternate gene names: 17230967

Gene position: 4194672-4196114 (Clockwise)

Preceding gene: 17230966

Following gene: 17230968

Centisome position: 65.4

GC content: 40.4

Gene sequence:

>1443_bases
ATGTCAATTAGCACTACAATTAAGACTATTCAAGATATCATGCGGAAAGATGCGGGCGTTGATGGTGACGCGCAACGCAT
TAACCAGTTAGTTTGGATGATATTCCTCAAGGTTTTTGATGCCCGTGAAGAAGAATATGAACTGTTAGAAGATAATTATA
AATCTCCCATTCCTGAAGGTTTGCGTTGGCGGAACTGGGCAGCAGATTCCGAAGGTATCACAGGAGATGGTTTACTAGAT
TTTGTAGATAATGCTTTATTTAAAACCCTTAAAGAACTAAGAACTACGGCAACTGATGCCCGTGGGCAGATGATTGGTAA
AGTGTTTGAGGATGCCTACAACTATATGAAAAATGGCACTCTCATCCGCCAAGTAATTAATAAGCTCAATGAAGTTGATT
TTAATAAAAAAGACCAGAAAAAACAGTTTAGCGAAATTTACGAGAAAATTCTCAAAGATTTGCAGAGTGCGGGAAATGCC
GGAGAATATTATACTCCCCGTGCGGTGACAAAGTTTATTGTAGATAGAATCAAGCCGCAATTAGGGGAAATTGTATTTGA
TCCAGCTTGCGGTACAGGTGGCTTTTTAACAGCAGCAATTGATTACATTCGCCAACATTTTCAAAGTGCTGATGTACCAG
AAATTCTGCAACGGACGATTCGCGGAACTGAGAAAAAGCCGTTACCTTTTAACCTTTGTGTCACGAATCTGATTTTAAAT
GGTATTGATGTGCCGTCAGCAGAACATGATAATACTTTGGCACGACCATTGCGCGATTACAGTCCTCACGAACGGGTGGA
TGTAATTATCACTAATCCGCCTTTTGGTGGGATGGAAGAAGATGGAATTGAGGACAATTTCCCAGCTACCTTCCGCACCA
GAGAAACGGCAGATTTATTTCTGGTGCTGATTGCTCATTTACTCAAGGAAGGCGGTAGAGGTGCAATAGTTTTACCAGAT
GGTACGCTGTTTGGCGAAGGTGTAAAAACGCGGATTAAAGAAAAACTCCTGCAAGATTGCAATCTGCATACAATTGTGCG
CTTACCAAATGGCGTATTTAATCCCTACACAGGTATTAAAACTAATCTGCTATTTTTCACTAAAGGGGAACCCACAGAAA
CGATTTGGTATTATGAACACCCTTACCCAGCAGGTTATAAGTCATACTCGAAAACTAAGCCGATTCGCTTTGAGGAGTTT
GCACCGGAGCAAGAATGGTGGGATAACCGCGAGGAAAATGAATTTGCTTGGAAAGTTTCAATTGCAGATTTGAAGGCGAA
TAATTACAACCTAGATATTAAAAATCCTCACAAAGTTGATGTAGAACACGCTGATTTAGATGAAATGTTAGCAGATCATC
AAAAACTTATGGCTGAATTGGGTGAGGTGCGGAGTAAGTTGAAATTTGAATTAATGGAAGCGTTGGAAAGAGATGAAGCT
TGA

Upstream 100 bases:

>100_bases
CCAGGGAGAGGTGCTTCACAGGAAAAAAGCGAAACTTCTGCATCACAATTATCTGCACCTACAGAAGAAGATAAAGAAAA
CGATAAAAGTTAAGTAAGCA

Downstream 100 bases:

>100_bases
AAGTTTTTTTGATAATTTTGAATTGTTAACTGATGCGCCAAATGCGAGCGCAAAGCTGCGGGATTTGATTTTGCAATTGG
CTGTTAAAGGTAAGCTAGTT

Product: type I restriction modification enzyme M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSISTTIKTIQDIMRKDAGVDGDAQRINQLVWMIFLKVFDAREEEYELLEDNYKSPIPEGLRWRNWAADSEGITGDGLLD
FVDNALFKTLKELRTTATDARGQMIGKVFEDAYNYMKNGTLIRQVINKLNEVDFNKKDQKKQFSEIYEKILKDLQSAGNA
GEYYTPRAVTKFIVDRIKPQLGEIVFDPACGTGGFLTAAIDYIRQHFQSADVPEILQRTIRGTEKKPLPFNLCVTNLILN
GIDVPSAEHDNTLARPLRDYSPHERVDVIITNPPFGGMEEDGIEDNFPATFRTRETADLFLVLIAHLLKEGGRGAIVLPD
GTLFGEGVKTRIKEKLLQDCNLHTIVRLPNGVFNPYTGIKTNLLFFTKGEPTETIWYYEHPYPAGYKSYSKTKPIRFEEF
APEQEWWDNREENEFAWKVSIADLKANNYNLDIKNPHKVDVEHADLDEMLADHQKLMAELGEVRSKLKFELMEALERDEA

Sequences:

>Translated_480_residues
MSISTTIKTIQDIMRKDAGVDGDAQRINQLVWMIFLKVFDAREEEYELLEDNYKSPIPEGLRWRNWAADSEGITGDGLLD
FVDNALFKTLKELRTTATDARGQMIGKVFEDAYNYMKNGTLIRQVINKLNEVDFNKKDQKKQFSEIYEKILKDLQSAGNA
GEYYTPRAVTKFIVDRIKPQLGEIVFDPACGTGGFLTAAIDYIRQHFQSADVPEILQRTIRGTEKKPLPFNLCVTNLILN
GIDVPSAEHDNTLARPLRDYSPHERVDVIITNPPFGGMEEDGIEDNFPATFRTRETADLFLVLIAHLLKEGGRGAIVLPD
GTLFGEGVKTRIKEKLLQDCNLHTIVRLPNGVFNPYTGIKTNLLFFTKGEPTETIWYYEHPYPAGYKSYSKTKPIRFEEF
APEQEWWDNREENEFAWKVSIADLKANNYNLDIKNPHKVDVEHADLDEMLADHQKLMAELGEVRSKLKFELMEALERDEA
>Mature_479_residues
SISTTIKTIQDIMRKDAGVDGDAQRINQLVWMIFLKVFDAREEEYELLEDNYKSPIPEGLRWRNWAADSEGITGDGLLDF
VDNALFKTLKELRTTATDARGQMIGKVFEDAYNYMKNGTLIRQVINKLNEVDFNKKDQKKQFSEIYEKILKDLQSAGNAG
EYYTPRAVTKFIVDRIKPQLGEIVFDPACGTGGFLTAAIDYIRQHFQSADVPEILQRTIRGTEKKPLPFNLCVTNLILNG
IDVPSAEHDNTLARPLRDYSPHERVDVIITNPPFGGMEEDGIEDNFPATFRTRETADLFLVLIAHLLKEGGRGAIVLPDG
TLFGEGVKTRIKEKLLQDCNLHTIVRLPNGVFNPYTGIKTNLLFFTKGEPTETIWYYEHPYPAGYKSYSKTKPIRFEEFA
PEQEWWDNREENEFAWKVSIADLKANNYNLDIKNPHKVDVEHADLDEMLADHQKLMAELGEVRSKLKFELMEALERDEA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=434, Percent_Identity=28.110599078341, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54925; Mature: 54794

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure 
SISTTIKTIQDIMRKDAGVDGDAQRINQLVWMIFLKVFDAREEEYELLEDNYKSPIPEG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
LRWRNWAADSEGITGDGLLDFVDNALFKTLKELRTTATDARGQMIGKVFEDAYNYMKNGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LIRQVINKLNEVDFNKKDQKKQFSEIYEKILKDLQSAGNAGEYYTPRAVTKFIVDRIKPQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LGEIVFDPACGTGGFLTAAIDYIRQHFQSADVPEILQRTIRGTEKKPLPFNLCVTNLILN
HCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GIDVPSAEHDNTLARPLRDYSPHERVDVIITNPPFGGMEEDGIEDNFPATFRTRETADLF
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCHHHHHH
LVLIAHLLKEGGRGAIVLPDGTLFGEGVKTRIKEKLLQDCNLHTIVRLPNGVFNPYTGIK
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCE
TNLLFFTKGEPTETIWYYEHPYPAGYKSYSKTKPIRFEEFAPEQEWWDNREENEFAWKVS
EEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCEEEEEE
IADLKANNYNLDIKNPHKVDVEHADLDEMLADHQKLMAELGEVRSKLKFELMEALERDEA
EEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]