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The map label for this gene is rihA [H]

Identifier: 17230777

GI number: 17230777

Start: 3960134

End: 3961057

Strand: Direct

Name: rihA [H]

Synonym: alr3285

Alternate gene names: 17230777

Gene position: 3960134-3961057 (Clockwise)

Preceding gene: 17230775

Following gene: 17230779

Centisome position: 61.74

GC content: 41.99

Gene sequence:

>924_bases
ATGACAAAACAACTAGTGTTAATGGATCATGATGGTGGTGTAGATGATTATCTAGCAACTATGCTGCTGTTGACGATGGA
TAATGTGCAACTCATGGGTGTTGTTGTTACTCCTGCTGATTGTTATGTCCAGCCTGCGGTGAGTGCTACGCGTAAAATTA
TTGATTTGATGGGATTTTCTCATATCCCAGTGGCAGAAAGTACTGTACGCGGGATTAATCCGTTTCCTCGGCTGTATCGG
CGTGATTCGTTTATTGTTGACCATCTGCCTATTCTCAATCAAACCGAATTGATTCATACACCTTTGGTTGAGGAAACTGG
TCAAGATTTTATGGTCAGAGTATTGCAAGAAGCACTAGAACCCGTAACATTAATGGTTACAGGCCCACTCACAACGGTTG
CAGTTGCTTTAGACCAAGCACCAGACATTGAAGCCAAGATTGACAAGATTGTCTGGATGGGTGGCGCTTTGAATGTTCCT
GGTAATGTGGAAAAAAGTTTAGAGGCTGGACAAGATGGGTCTGCCGAATGGAATGTCTATTGGGATGCAGTTTCAGCTGC
ACGAGTATGGCAAAGGGAAATTAAGATTATTATGTGTCCCTTAGATTTGACTAACGATGTACCAGTGACATCTGAGTTAG
TATACAAAATGGGGCGACAAAGACATTATCCCGTATCTGATTTAGCTGGACAATGTTATGCCTTAGTTATCCCACAAGAT
TATTATTTCTGGGATGTGTTAGCAACGGCTTATTTAGGACACCCAGAATTTTATCAACTCCGCGAATGGGAGACAGAAAT
TATTACTAGTGGTTTGAGTCAGGGACGGACTAAAGTTGTCACTGGTGGGCGCAAGATTTTAGCTATGGATAAAGTGGATA
AGGATGCTTTTTATGCTTACATTTTGCAACAGTGGGCAAGGTAA

Upstream 100 bases:

>100_bases
GACAAGCACGGAACCAATGGGAATACCTCCTTCTTGTCTACCTTGTTTTGCTTCTTGAATGGCGGCCTGCATAAACTCAT
CCATTTTTTATCCTCCTTAT

Downstream 100 bases:

>100_bases
ACAATTCAAAATACCCTAAGGGAAGCTAAAGCTACAAAATTCAAAATTCAAAAAAATGGAAATTTGAGGACTTGACATCC
TCCACAAAGTCCGATCAAGC

Product: inosine-adenosine-guanosine-nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTKQLVLMDHDGGVDDYLATMLLLTMDNVQLMGVVVTPADCYVQPAVSATRKIIDLMGFSHIPVAESTVRGINPFPRLYR
RDSFIVDHLPILNQTELIHTPLVEETGQDFMVRVLQEALEPVTLMVTGPLTTVAVALDQAPDIEAKIDKIVWMGGALNVP
GNVEKSLEAGQDGSAEWNVYWDAVSAARVWQREIKIIMCPLDLTNDVPVTSELVYKMGRQRHYPVSDLAGQCYALVIPQD
YYFWDVLATAYLGHPEFYQLREWETEIITSGLSQGRTKVVTGGRKILAMDKVDKDAFYAYILQQWAR

Sequences:

>Translated_307_residues
MTKQLVLMDHDGGVDDYLATMLLLTMDNVQLMGVVVTPADCYVQPAVSATRKIIDLMGFSHIPVAESTVRGINPFPRLYR
RDSFIVDHLPILNQTELIHTPLVEETGQDFMVRVLQEALEPVTLMVTGPLTTVAVALDQAPDIEAKIDKIVWMGGALNVP
GNVEKSLEAGQDGSAEWNVYWDAVSAARVWQREIKIIMCPLDLTNDVPVTSELVYKMGRQRHYPVSDLAGQCYALVIPQD
YYFWDVLATAYLGHPEFYQLREWETEIITSGLSQGRTKVVTGGRKILAMDKVDKDAFYAYILQQWAR
>Mature_306_residues
TKQLVLMDHDGGVDDYLATMLLLTMDNVQLMGVVVTPADCYVQPAVSATRKIIDLMGFSHIPVAESTVRGINPFPRLYRR
DSFIVDHLPILNQTELIHTPLVEETGQDFMVRVLQEALEPVTLMVTGPLTTVAVALDQAPDIEAKIDKIVWMGGALNVPG
NVEKSLEAGQDGSAEWNVYWDAVSAARVWQREIKIIMCPLDLTNDVPVTSELVYKMGRQRHYPVSDLAGQCYALVIPQDY
YFWDVLATAYLGHPEFYQLREWETEIITSGLSQGRTKVVTGGRKILAMDKVDKDAFYAYILQQWAR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=316, Percent_Identity=26.5822784810127, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788486, Length=298, Percent_Identity=24.496644295302, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786213, Length=172, Percent_Identity=30.2325581395349, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34478; Mature: 34347

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQLVLMDHDGGVDDYLATMLLLTMDNVQLMGVVVTPADCYVQPAVSATRKIIDLMGFS
CCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEECCHHHEECHHHHHHHHHHHHHCCC
HIPVAESTVRGINPFPRLYRRDSFIVDHLPILNQTELIHTPLVEETGQDFMVRVLQEALE
CCCCCHHHHCCCCHHHHHHCCCCEEEECCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHCC
PVTLMVTGPLTTVAVALDQAPDIEAKIDKIVWMGGALNVPGNVEKSLEAGQDGSAEWNVY
CEEEEEECCHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEE
WDAVSAARVWQREIKIIMCPLDLTNDVPVTSELVYKMGRQRHYPVSDLAGQCYALVIPQD
ECHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCC
YYFWDVLATAYLGHPEFYQLREWETEIITSGLSQGRTKVVTGGRKILAMDKVDKDAFYAY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHH
ILQQWAR
HHHHHCC
>Mature Secondary Structure 
TKQLVLMDHDGGVDDYLATMLLLTMDNVQLMGVVVTPADCYVQPAVSATRKIIDLMGFS
CCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEECCHHHEECHHHHHHHHHHHHHCCC
HIPVAESTVRGINPFPRLYRRDSFIVDHLPILNQTELIHTPLVEETGQDFMVRVLQEALE
CCCCCHHHHCCCCHHHHHHCCCCEEEECCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHCC
PVTLMVTGPLTTVAVALDQAPDIEAKIDKIVWMGGALNVPGNVEKSLEAGQDGSAEWNVY
CEEEEEECCHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCCCCHHHHHCCCCCCCEEEEE
WDAVSAARVWQREIKIIMCPLDLTNDVPVTSELVYKMGRQRHYPVSDLAGQCYALVIPQD
ECHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCC
YYFWDVLATAYLGHPEFYQLREWETEIITSGLSQGRTKVVTGGRKILAMDKVDKDAFYAY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHH
ILQQWAR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA