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The map label for this gene is trpE [H]

Identifier: 17230725

GI number: 17230725

Start: 3904984

End: 3907131

Strand: Direct

Name: trpE [H]

Synonym: alr3233

Alternate gene names: 17230725

Gene position: 3904984-3907131 (Clockwise)

Preceding gene: 17230723

Following gene: 17230726

Centisome position: 60.88

GC content: 45.76

Gene sequence:

>2148_bases
GTGCGCGTATCTCGTAGCACAACCGAGGTGAAGATGGACACTGCACTAGATGAAATTCTCTTTCACCTAAATCAAGTACG
TGGAGGTTTGTTAACCAGTAGTTACGAATATCCAGGGCGATACAAAAGATGGGCGATTGGATTCATTAATCCCCCATTAC
AACTGACAACAAGAGAGAACGCATTTACCATCTCTTCACTCAATCCTCGCGGACAGGTGCTACTACCAACCTTGTTCCAG
CATCTATCAGCCCAGTCGCAACTACAACAAATCAGCCTCAATCATGACTACATCACAGGTGAAATTCGACCCACAAAACA
GTTATTCACAGAAGAACAACGGAGTAAACAACCGTCAGCCTTTACAGTCATCCGCGAAATTCTCCAGATTTTTGCGAGTG
ATGAAGACGAGCATTTAGGGTTATATGGTGCATTTGGTTACGACTTAGTATTTCAATTTGAACCAATTCCCCAAAAAATT
GCTCGTCCCGCAGACCAACGGGATTTAGTCCTGTATCTACCCGATGAACTCATAGTTGTAGATTACTATCTACAAAAAGC
ATATCGTCACCAGTATGAATTTGCCACAGAACATGGCAACACCGAGCATCTTCCACGGACAGGCCAGTCCATCGACTACC
AGGGTAAACATCTTCTACCAAACCAAACTGCTGACCATCAACCAGGAGAATATGCCAACCTAGTTGAGCAAGCACTCGAC
TACTTCCGCCGGGGTGACTTATTTGAAGTAGTTCCTAGTCAAAACTTTTTTACAGCCTGTGAACAATCACCCAGTCAACT
ATTCCAGACCTTAAGGCAAATTAATCCTAGTCCTTATGGATTTCTGTTGAATTTGGGTGGTGAATATCTCATAGGTGCAT
CACCAGAAATGTTTGTGCGAGTTGATGGTAGGCGAGTGGAAACCTGTCCCATTAGTGGCACTATTAGACGGGGAGAAGAT
GCTTTAGGCGACGCTGTACAAATTCGTCAGTTGCTTAACTCCCATAAAGATGAAGCCGAGTTAACAATGTGTACTGACGT
AGACCGCAACGATAAATCGCGGATTTGTGAACCCGGTTCAGTCAGGGTGATTGGTCGTCGCCAGATTGAACTGTACAGCC
ACCTCATTCATACAGTAGACCATGTAGAAGGGATACTGAGGCCGGAATTTGACGCTTTAGATGCCTTCTTGAGTCATACT
TGGGCAGTTACAGTCACAGGCGCACCCAAACGAGCCGCCATGCAGTTCATCGAACAGCATGAACGCAGCGCCCGTCGTTG
GTATGGGGGAGCAGTTGGTTATTTAGGCTTTAATGGTAACTTGAATACCGGATTAACCTTGCGGACAATTCGTTTACAAG
ACTCCATCGCCGAAGTGCGAGTTGGTGCAACAGTCCTTTACGACTCCATTCCGTCAGCCGAAGAAGAGGAAACAATTACT
AAAGCGACTGCATTATTTGAGACCATTCGCCGTCATACCACTGCCAATAAAACTCAAGGAAACGATAGTCATCGCCCTGG
GGATATCGCCCACAATAAGCGTATCCTCCTCATCGACTACGAAGATTCATTTGTTCACACATTAGCCAATTACATCCGCA
CCACCGGCGCAACCGTCACCACCCTACGTCATGGTTTTGCTGAATCATATTTTGATGCAGAACGCCCAGACTTAGTGGTA
TTGTCTCCCGGCCCTGGTAGACCCAGTGACTTCCGAGTTCCCCAAACGGTTGCAGCCTTGGTAGGTCGAGAAATCCCCAT
TTTTGGCGTTTGTCTGGGATTACAAGGCATAGTGGAAGCTTTTGGCGGAGAATTAGGCGTGCTTGATTATCCCCAACACG
GTAAACCCGCACGGATTTCAGTGACTGCACCTGATTCTGTGCTGTTTCAAAATTTACCAGCATCCTTCATCGTGGGTAGA
TACCATTCCTTATTTGCCCAACCCCAAACTATACCCGGTGAACTCAAAGTCACAGCGATTTCTGAGGACAATGTAATTAT
GGCAATTGAACACCAAACACTACCTATAGCCGCCGTCCAATTTCATCCAGAGTCAATCATGACCCTAGCAGGAGAAGTTG
GTCAGACAATCATTAAAAATGTGGTGCAGACATATACCCAAACTTTAGAAACATCAATTTACTCTTAG

Upstream 100 bases:

>100_bases
GCCAAAAGTAGGGCGAACCGTTCGCGCTATACTCCCTCCAACACACAGAAAAGAAAAGACAATGAATTACTATACACTTG
CTTACACAACCCTTGGTAAC

Downstream 100 bases:

>100_bases
TGGTAATGGGTAATATAGCTGTTGACAGTTAACTGTTGACAGTTGACTGTTGACAGATTTGAAAGTCTTGTTTTATCAGG
GTTTGATGTTAGCTGGATGT

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MRVSRSTTEVKMDTALDEILFHLNQVRGGLLTSSYEYPGRYKRWAIGFINPPLQLTTRENAFTISSLNPRGQVLLPTLFQ
HLSAQSQLQQISLNHDYITGEIRPTKQLFTEEQRSKQPSAFTVIREILQIFASDEDEHLGLYGAFGYDLVFQFEPIPQKI
ARPADQRDLVLYLPDELIVVDYYLQKAYRHQYEFATEHGNTEHLPRTGQSIDYQGKHLLPNQTADHQPGEYANLVEQALD
YFRRGDLFEVVPSQNFFTACEQSPSQLFQTLRQINPSPYGFLLNLGGEYLIGASPEMFVRVDGRRVETCPISGTIRRGED
ALGDAVQIRQLLNSHKDEAELTMCTDVDRNDKSRICEPGSVRVIGRRQIELYSHLIHTVDHVEGILRPEFDALDAFLSHT
WAVTVTGAPKRAAMQFIEQHERSARRWYGGAVGYLGFNGNLNTGLTLRTIRLQDSIAEVRVGATVLYDSIPSAEEEETIT
KATALFETIRRHTTANKTQGNDSHRPGDIAHNKRILLIDYEDSFVHTLANYIRTTGATVTTLRHGFAESYFDAERPDLVV
LSPGPGRPSDFRVPQTVAALVGREIPIFGVCLGLQGIVEAFGGELGVLDYPQHGKPARISVTAPDSVLFQNLPASFIVGR
YHSLFAQPQTIPGELKVTAISEDNVIMAIEHQTLPIAAVQFHPESIMTLAGEVGQTIIKNVVQTYTQTLETSIYS

Sequences:

>Translated_715_residues
MRVSRSTTEVKMDTALDEILFHLNQVRGGLLTSSYEYPGRYKRWAIGFINPPLQLTTRENAFTISSLNPRGQVLLPTLFQ
HLSAQSQLQQISLNHDYITGEIRPTKQLFTEEQRSKQPSAFTVIREILQIFASDEDEHLGLYGAFGYDLVFQFEPIPQKI
ARPADQRDLVLYLPDELIVVDYYLQKAYRHQYEFATEHGNTEHLPRTGQSIDYQGKHLLPNQTADHQPGEYANLVEQALD
YFRRGDLFEVVPSQNFFTACEQSPSQLFQTLRQINPSPYGFLLNLGGEYLIGASPEMFVRVDGRRVETCPISGTIRRGED
ALGDAVQIRQLLNSHKDEAELTMCTDVDRNDKSRICEPGSVRVIGRRQIELYSHLIHTVDHVEGILRPEFDALDAFLSHT
WAVTVTGAPKRAAMQFIEQHERSARRWYGGAVGYLGFNGNLNTGLTLRTIRLQDSIAEVRVGATVLYDSIPSAEEEETIT
KATALFETIRRHTTANKTQGNDSHRPGDIAHNKRILLIDYEDSFVHTLANYIRTTGATVTTLRHGFAESYFDAERPDLVV
LSPGPGRPSDFRVPQTVAALVGREIPIFGVCLGLQGIVEAFGGELGVLDYPQHGKPARISVTAPDSVLFQNLPASFIVGR
YHSLFAQPQTIPGELKVTAISEDNVIMAIEHQTLPIAAVQFHPESIMTLAGEVGQTIIKNVVQTYTQTLETSIYS
>Mature_715_residues
MRVSRSTTEVKMDTALDEILFHLNQVRGGLLTSSYEYPGRYKRWAIGFINPPLQLTTRENAFTISSLNPRGQVLLPTLFQ
HLSAQSQLQQISLNHDYITGEIRPTKQLFTEEQRSKQPSAFTVIREILQIFASDEDEHLGLYGAFGYDLVFQFEPIPQKI
ARPADQRDLVLYLPDELIVVDYYLQKAYRHQYEFATEHGNTEHLPRTGQSIDYQGKHLLPNQTADHQPGEYANLVEQALD
YFRRGDLFEVVPSQNFFTACEQSPSQLFQTLRQINPSPYGFLLNLGGEYLIGASPEMFVRVDGRRVETCPISGTIRRGED
ALGDAVQIRQLLNSHKDEAELTMCTDVDRNDKSRICEPGSVRVIGRRQIELYSHLIHTVDHVEGILRPEFDALDAFLSHT
WAVTVTGAPKRAAMQFIEQHERSARRWYGGAVGYLGFNGNLNTGLTLRTIRLQDSIAEVRVGATVLYDSIPSAEEEETIT
KATALFETIRRHTTANKTQGNDSHRPGDIAHNKRILLIDYEDSFVHTLANYIRTTGATVTTLRHGFAESYFDAERPDLVV
LSPGPGRPSDFRVPQTVAALVGREIPIFGVCLGLQGIVEAFGGELGVLDYPQHGKPARISVTAPDSVLFQNLPASFIVGR
YHSLFAQPQTIPGELKVTAISEDNVIMAIEHQTLPIAAVQFHPESIMTLAGEVGQTIIKNVVQTYTQTLETSIYS

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=410, Percent_Identity=33.1707317073171, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788114, Length=357, Percent_Identity=29.4117647058824, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1789760, Length=190, Percent_Identity=37.3684210526316, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787517, Length=183, Percent_Identity=37.1584699453552, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786809, Length=232, Percent_Identity=31.0344827586207, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI87082077, Length=272, Percent_Identity=24.6323529411765, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=375, Percent_Identity=34.4, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322638, Length=201, Percent_Identity=36.318407960199, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324361, Length=268, Percent_Identity=24.2537313432836, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 80168; Mature: 80168

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRSTTEVKMDTALDEILFHLNQVRGGLLTSSYEYPGRYKRWAIGFINPPLQLTTREN
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCEEEEECCC
AFTISSLNPRGQVLLPTLFQHLSAQSQLQQISLNHDYITGEIRPTKQLFTEEQRSKQPSA
EEEEECCCCCCCEEHHHHHHHHCHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHH
FTVIREILQIFASDEDEHLGLYGAFGYDLVFQFEPIPQKIARPADQRDLVLYLPDELIVV
HHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCCHHHCCCCCCCCEEEECCCCEEEH
DYYLQKAYRHQYEFATEHGNTEHLPRTGQSIDYQGKHLLPNQTADHQPGEYANLVEQALD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YFRRGDLFEVVPSQNFFTACEQSPSQLFQTLRQINPSPYGFLLNLGGEYLIGASPEMFVR
HHHCCCEEEECCCCCHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEE
VDGRRVETCPISGTIRRGEDALGDAVQIRQLLNSHKDEAELTMCTDVDRNDKSRICEPGS
ECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHCCCCCCC
VRVIGRRQIELYSHLIHTVDHVEGILRPEFDALDAFLSHTWAVTVTGAPKRAAMQFIEQH
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
ERSARRWYGGAVGYLGFNGNLNTGLTLRTIRLQDSIAEVRVGATVLYDSIPSAEEEETIT
HHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHCCEEEECCCCCCCHHHHHH
KATALFETIRRHTTANKTQGNDSHRPGDIAHNKRILLIDYEDSFVHTLANYIRTTGATVT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
TLRHGFAESYFDAERPDLVVLSPGPGRPSDFRVPQTVAALVGREIPIFGVCLGLQGIVEA
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
FGGELGVLDYPQHGKPARISVTAPDSVLFQNLPASFIVGRYHSLFAQPQTIPGELKVTAI
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
SEDNVIMAIEHQTLPIAAVQFHPESIMTLAGEVGQTIIKNVVQTYTQTLETSIYS
CCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSRSTTEVKMDTALDEILFHLNQVRGGLLTSSYEYPGRYKRWAIGFINPPLQLTTREN
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCEEEEECCC
AFTISSLNPRGQVLLPTLFQHLSAQSQLQQISLNHDYITGEIRPTKQLFTEEQRSKQPSA
EEEEECCCCCCCEEHHHHHHHHCHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHH
FTVIREILQIFASDEDEHLGLYGAFGYDLVFQFEPIPQKIARPADQRDLVLYLPDELIVV
HHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCCHHHCCCCCCCCEEEECCCCEEEH
DYYLQKAYRHQYEFATEHGNTEHLPRTGQSIDYQGKHLLPNQTADHQPGEYANLVEQALD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YFRRGDLFEVVPSQNFFTACEQSPSQLFQTLRQINPSPYGFLLNLGGEYLIGASPEMFVR
HHHCCCEEEECCCCCHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEE
VDGRRVETCPISGTIRRGEDALGDAVQIRQLLNSHKDEAELTMCTDVDRNDKSRICEPGS
ECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHCCCCCCC
VRVIGRRQIELYSHLIHTVDHVEGILRPEFDALDAFLSHTWAVTVTGAPKRAAMQFIEQH
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
ERSARRWYGGAVGYLGFNGNLNTGLTLRTIRLQDSIAEVRVGATVLYDSIPSAEEEETIT
HHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHCCEEEECCCCCCCHHHHHH
KATALFETIRRHTTANKTQGNDSHRPGDIAHNKRILLIDYEDSFVHTLANYIRTTGATVT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
TLRHGFAESYFDAERPDLVVLSPGPGRPSDFRVPQTVAALVGREIPIFGVCLGLQGIVEA
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
FGGELGVLDYPQHGKPARISVTAPDSVLFQNLPASFIVGRYHSLFAQPQTIPGELKVTAI
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
SEDNVIMAIEHQTLPIAAVQFHPESIMTLAGEVGQTIIKNVVQTYTQTLETSIYS
CCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]