Click here to switch to the map view.

The map label for this gene is ispE [H]

Identifier: 17230722

GI number: 17230722

Start: 3900913

End: 3901866

Strand: Direct

Name: ispE [H]

Synonym: alr3230

Alternate gene names: 17230722

Gene position: 3900913-3901866 (Clockwise)

Preceding gene: 17230721

Following gene: 17230723

Centisome position: 60.82

GC content: 44.13

Gene sequence:

>954_bases
ATGCGTTCCTACAAATTAATTGCTCCCGCCAAAATTAACTTGTATCTAGAAATCATCGGCGACAGACCCGATGGGTATCA
TGAGTTAGTGATGATATTGCAAAGTATCGACCTAGCCGACGAGATTGAAATACATTCCCTCAGCAGTGAAACTATCCACG
TTCATTGCAACCACCCACAAGTTCCCACAGACAAAAGTAATCTCGTATATCGCGCCGCCGAACTGATGGTGACAAGATTT
CCTGAAGCCTTCACGAAACATGGGGGTGTAGATATTACCGTTCACAAACATATTCCCGTAGCAGCTGGCTTGGCTGGAGG
TTCAACTAACGCCGCAGCTGTGTTAGTCGGGATAGATTTACTTTGGAACTTGGGACTAACCCAAACAGAACTAGAAGAGT
TAGGCTCTACTCTTGGTTCCGACGTACCATTTTGTGTGGCTGGTGGTACAGTCATCGCCACAGGTAGAGGAGAACAACTT
TCCCCCTTGCCCAGTTTGGATCATATATATATAGTATTAGGCAAATATCGCAGCTTAGAAGTTTCCACCGCCTGGGCATA
TAAAAACTACCGTCAAGAATATGGTAGTACTTATCTTAGAGATACCAATGACTTAGCCTCCCGCGCCGCCGCAGTTCATT
CAGGTAGTATAGTTAAGGCTATTGTAGAAAAAGATGCTGTAGCGATCGCCCAAAGACTGCACAATGATTTAGAGAAAGTA
GTATTACCTTCCTATCCGCAAGTTTTGCACCTCCGAGAATTGTTAGCATCTCAACCAGGTGTTATCGGAACCATGATGTC
TGGTTCAGGCCCTTCAGTATTTGCTCTGTGTGAAACTCAAGCACAAGCAGAACAAGTTCAGCAGCAAGTAAGACAAACAA
TTCCCGACGAAGATTTAGAATTATTTGTAACTCGCACAATTACACATGGGATTCAAGTTGTGGGTAATGGGTAA

Upstream 100 bases:

>100_bases
AAAATTAGCAAACTTGTTGGGAGTAGAGTAATTTCAGATTTTGGATTTTCGATAAAAGTCAAATCCCCGTCCCCAGTCCC
CAGTCCCCAATTCCCATTCC

Downstream 100 bases:

>100_bases
CTTATGAACAACGAAACTCAACAAACAGATACATCAGGACAAGTTCCACCTACGCCATTACGCTGCATAACAGGAGCAGG
TATTTCTGGCGGAATGGGAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRSYKLIAPAKINLYLEIIGDRPDGYHELVMILQSIDLADEIEIHSLSSETIHVHCNHPQVPTDKSNLVYRAAELMVTRF
PEAFTKHGGVDITVHKHIPVAAGLAGGSTNAAAVLVGIDLLWNLGLTQTELEELGSTLGSDVPFCVAGGTVIATGRGEQL
SPLPSLDHIYIVLGKYRSLEVSTAWAYKNYRQEYGSTYLRDTNDLASRAAAVHSGSIVKAIVEKDAVAIAQRLHNDLEKV
VLPSYPQVLHLRELLASQPGVIGTMMSGSGPSVFALCETQAQAEQVQQQVRQTIPDEDLELFVTRTITHGIQVVGNG

Sequences:

>Translated_317_residues
MRSYKLIAPAKINLYLEIIGDRPDGYHELVMILQSIDLADEIEIHSLSSETIHVHCNHPQVPTDKSNLVYRAAELMVTRF
PEAFTKHGGVDITVHKHIPVAAGLAGGSTNAAAVLVGIDLLWNLGLTQTELEELGSTLGSDVPFCVAGGTVIATGRGEQL
SPLPSLDHIYIVLGKYRSLEVSTAWAYKNYRQEYGSTYLRDTNDLASRAAAVHSGSIVKAIVEKDAVAIAQRLHNDLEKV
VLPSYPQVLHLRELLASQPGVIGTMMSGSGPSVFALCETQAQAEQVQQQVRQTIPDEDLELFVTRTITHGIQVVGNG
>Mature_317_residues
MRSYKLIAPAKINLYLEIIGDRPDGYHELVMILQSIDLADEIEIHSLSSETIHVHCNHPQVPTDKSNLVYRAAELMVTRF
PEAFTKHGGVDITVHKHIPVAAGLAGGSTNAAAVLVGIDLLWNLGLTQTELEELGSTLGSDVPFCVAGGTVIATGRGEQL
SPLPSLDHIYIVLGKYRSLEVSTAWAYKNYRQEYGSTYLRDTNDLASRAAAVHSGSIVKAIVEKDAVAIAQRLHNDLEKV
VLPSYPQVLHLRELLASQPGVIGTMMSGSGPSVFALCETQAQAEQVQQQVRQTIPDEDLELFVTRTITHGIQVVGNG

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=293, Percent_Identity=30.7167235494881, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 34407; Mature: 34407

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYKLIAPAKINLYLEIIGDRPDGYHELVMILQSIDLADEIEIHSLSSETIHVHCNHPQ
CCCCEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCC
VPTDKSNLVYRAAELMVTRFPEAFTKHGGVDITVHKHIPVAAGLAGGSTNAAAVLVGIDL
CCCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCEEEECCCCCCCCEEEEEEHHH
LWNLGLTQTELEELGSTLGSDVPFCVAGGTVIATGRGEQLSPLPSLDHIYIVLGKYRSLE
HHCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCEEE
VSTAWAYKNYRQEYGSTYLRDTNDLASRAAAVHSGSIVKAIVEKDAVAIAQRLHNDLEKV
EEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLPSYPQVLHLRELLASQPGVIGTMMSGSGPSVFALCETQAQAEQVQQQVRQTIPDEDLE
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHH
LFVTRTITHGIQVVGNG
EEEHEEHHCCEEEEECC
>Mature Secondary Structure
MRSYKLIAPAKINLYLEIIGDRPDGYHELVMILQSIDLADEIEIHSLSSETIHVHCNHPQ
CCCCEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCC
VPTDKSNLVYRAAELMVTRFPEAFTKHGGVDITVHKHIPVAAGLAGGSTNAAAVLVGIDL
CCCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCEEEECCCCCCCCEEEEEEHHH
LWNLGLTQTELEELGSTLGSDVPFCVAGGTVIATGRGEQLSPLPSLDHIYIVLGKYRSLE
HHCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCEEE
VSTAWAYKNYRQEYGSTYLRDTNDLASRAAAVHSGSIVKAIVEKDAVAIAQRLHNDLEKV
EEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLPSYPQVLHLRELLASQPGVIGTMMSGSGPSVFALCETQAQAEQVQQQVRQTIPDEDLE
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHH
LFVTRTITHGIQVVGNG
EEEHEEHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA