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The map label for this gene is wcaF [H]

Identifier: 17230670

GI number: 17230670

Start: 3846744

End: 3847307

Strand: Direct

Name: wcaF [H]

Synonym: alr3178

Alternate gene names: 17230670

Gene position: 3846744-3847307 (Clockwise)

Preceding gene: 17230669

Following gene: 17230674

Centisome position: 59.98

GC content: 48.76

Gene sequence:

>564_bases
ATGACTAACGACAAACCCTTTGTAGATTTACGCCAGTATGACCAATCTTGGTTTGATCGGGGGCGTTCAGCTTGGTATAT
TTTGTTATGGTGGTTGGTGCAGGCGATCGCATTTCCTCTGACTCCCCATTTTTCTAGTAACATCCGTTGTTGGATATTAC
GTCTGTTTGGTGCGCGGATTGGTAAAGGTGTGGTGGTTCGTCCAACTGCGCGTTTTACCTTTCCCTGGAAAGTCACCATC
GGAGATTACAGTTGGGTTGGCGATGATGTAGTTTTCTACAGTCTAGATGAGATTCACATCGGTCAACACTGCGTAATTTC
CCAGAAAAGTTACCTCTGTACAGGTAGCCATGATATCCAAGACCCGACATTCAGACTAAAGGTAGCAGGGATTACCATTG
GTAACGGTGCTTGGGTCGCTACAGATTGTTTCATCGCGCCAGGGGTGCAAATAGGAGCTAACGCAGTGATTGGCGCTCGC
AGCAGCGTTTTTACTAGTATGCCGACTGGGCAGGTTTGCTGGGGTAGCCCTTGCCGTCCCCAGCATCCCAGGTTGAAGGA
GTGA

Upstream 100 bases:

>100_bases
TTAAATACTGCAAATACATCTAATCCTATCCCGCCATCTCTCACCCAAGAAATCGAGAAATCTACAACTTAGAAGATGCG
GGGGAGATGACTGTTGACTA

Downstream 100 bases:

>100_bases
GGGGGAGTAGGGAAGCAGGGGAGGCAGGGGGGAATTGTTGAATTTTGAACTTTGAATTTTGAATTGATTCCTCCCCAATC
CCCTACTGAAGTTTACGAGA

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTNDKPFVDLRQYDQSWFDRGRSAWYILLWWLVQAIAFPLTPHFSSNIRCWILRLFGARIGKGVVVRPTARFTFPWKVTI
GDYSWVGDDVVFYSLDEIHIGQHCVISQKSYLCTGSHDIQDPTFRLKVAGITIGNGAWVATDCFIAPGVQIGANAVIGAR
SSVFTSMPTGQVCWGSPCRPQHPRLKE

Sequences:

>Translated_187_residues
MTNDKPFVDLRQYDQSWFDRGRSAWYILLWWLVQAIAFPLTPHFSSNIRCWILRLFGARIGKGVVVRPTARFTFPWKVTI
GDYSWVGDDVVFYSLDEIHIGQHCVISQKSYLCTGSHDIQDPTFRLKVAGITIGNGAWVATDCFIAPGVQIGANAVIGAR
SSVFTSMPTGQVCWGSPCRPQHPRLKE
>Mature_186_residues
TNDKPFVDLRQYDQSWFDRGRSAWYILLWWLVQAIAFPLTPHFSSNIRCWILRLFGARIGKGVVVRPTARFTFPWKVTIG
DYSWVGDDVVFYSLDEIHIGQHCVISQKSYLCTGSHDIQDPTFRLKVAGITIGNGAWVATDCFIAPGVQIGANAVIGARS
SVFTSMPTGQVCWGSPCRPQHPRLKE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=167, Percent_Identity=53.8922155688623, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1786664, Length=118, Percent_Identity=30.5084745762712, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 21079; Mature: 20948

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDKPFVDLRQYDQSWFDRGRSAWYILLWWLVQAIAFPLTPHFSSNIRCWILRLFGARI
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GKGVVVRPTARFTFPWKVTIGDYSWVGDDVVFYSLDEIHIGQHCVISQKSYLCTGSHDIQ
CCCEEEECCCEEEEEEEEEECCCEECCCCEEEEEECEEECCCEEEECCCCEEEECCCCCC
DPTFRLKVAGITIGNGAWVATDCFIAPGVQIGANAVIGARSSVFTSMPTGQVCWGSPCRP
CCEEEEEEEEEEECCCCEEEEEEEECCCCEECCCEEEECCCHHEECCCCCCCEECCCCCC
QHPRLKE
CCCCCCC
>Mature Secondary Structure 
TNDKPFVDLRQYDQSWFDRGRSAWYILLWWLVQAIAFPLTPHFSSNIRCWILRLFGARI
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
GKGVVVRPTARFTFPWKVTIGDYSWVGDDVVFYSLDEIHIGQHCVISQKSYLCTGSHDIQ
CCCEEEECCCEEEEEEEEEECCCEECCCCEEEEEECEEECCCEEEECCCCEEEECCCCCC
DPTFRLKVAGITIGNGAWVATDCFIAPGVQIGANAVIGARSSVFTSMPTGQVCWGSPCRP
CCEEEEEEEEEEECCCCEEEEEEEECCCCEECCCEEEECCCHHEECCCCCCCEECCCCCC
QHPRLKE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]