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The map label for this gene is miaE [H]

Identifier: 17230413

GI number: 17230413

Start: 3561840

End: 3562439

Strand: Direct

Name: miaE [H]

Synonym: alr2921

Alternate gene names: 17230413

Gene position: 3561840-3562439 (Clockwise)

Preceding gene: 17230412

Following gene: 17230414

Centisome position: 55.53

GC content: 45.0

Gene sequence:

>600_bases
GTGCTTACCCCCGTGTTACCAAAAATCAATGTACTAAAGCAAACTACGAGTGAGGCTTGGATAGAACAAGCGATCGCTAA
CTTAGATATCATATTGCTTGACCACTCCCACTGCGAACGCAAAGCGGCGGGTGTGGCTTTAAATTTTATGTTTCGCTACC
CATCCAATACCAAAATGGTAAGGGAATTAACGGCGATCGCCCGTGAAGAATTAGAACATTTTGAACTGGTAAATCAGTGG
CTAGAACGCCGCAATATACCCCTAGCACCCCTACCCCCTCCTCCCTATGGCGCTGGCTTAAAAGCGGCTGCACGTACCAA
CGAACCCGAACGCTTTTTAGATTCCTTACTCGTGACTGGTTTAATCGAAGCACGAAGTCACGAACGCTTAGGCTTATTAG
CTACACACTGTCCCGAACCAGAACTAGCCAAGTTTTATAAAGGTTTGATGGCCTCAGAGGCGCGACACTTTGGTACATAT
TGGGTTTTAGCGGATACTTACTTTGACCGGGAAATTGTTAGTCAAAGGCTCGATGAATTAGCTATTGTTGAAAGTGAGTT
ACTGGAAAATTTACACCCAGAACCTAGAATTCATAGTTAG

Upstream 100 bases:

>100_bases
AAAATATAACAGCACTTTTTTGACGCAGATAACCGAAAAGCCAGCCACTCACATTACAATCAATGTGCTGGAGTTACAAT
TTTGATAGTTTTTTCGTCCT

Downstream 100 bases:

>100_bases
TATAATCACCAATAAATATGCAGATACAGTTAACACATATCAGGTTGCTTGTCTCCAACTACAAAGATTGTTTTTTATTC
TATCGAGATGCACTCGGATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MLTPVLPKINVLKQTTSEAWIEQAIANLDIILLDHSHCERKAAGVALNFMFRYPSNTKMVRELTAIAREELEHFELVNQW
LERRNIPLAPLPPPPYGAGLKAAARTNEPERFLDSLLVTGLIEARSHERLGLLATHCPEPELAKFYKGLMASEARHFGTY
WVLADTYFDREIVSQRLDELAIVESELLENLHPEPRIHS

Sequences:

>Translated_199_residues
MLTPVLPKINVLKQTTSEAWIEQAIANLDIILLDHSHCERKAAGVALNFMFRYPSNTKMVRELTAIAREELEHFELVNQW
LERRNIPLAPLPPPPYGAGLKAAARTNEPERFLDSLLVTGLIEARSHERLGLLATHCPEPELAKFYKGLMASEARHFGTY
WVLADTYFDREIVSQRLDELAIVESELLENLHPEPRIHS
>Mature_199_residues
MLTPVLPKINVLKQTTSEAWIEQAIANLDIILLDHSHCERKAAGVALNFMFRYPSNTKMVRELTAIAREELEHFELVNQW
LERRNIPLAPLPPPPYGAGLKAAARTNEPERFLDSLLVTGLIEARSHERLGLLATHCPEPELAKFYKGLMASEARHFGTY
WVLADTYFDREIVSQRLDELAIVESELLENLHPEPRIHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 22685; Mature: 22685

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPVLPKINVLKQTTSEAWIEQAIANLDIILLDHSHCERKAAGVALNFMFRYPSNTKMV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCEEEEEEEECCCCHHHH
RELTAIAREELEHFELVNQWLERRNIPLAPLPPPPYGAGLKAAARTNEPERFLDSLLVTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
LIEARSHERLGLLATHCPEPELAKFYKGLMASEARHFGTYWVLADTYFDREIVSQRLDEL
HHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHH
AIVESELLENLHPEPRIHS
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTPVLPKINVLKQTTSEAWIEQAIANLDIILLDHSHCERKAAGVALNFMFRYPSNTKMV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCEEEEEEEECCCCHHHH
RELTAIAREELEHFELVNQWLERRNIPLAPLPPPPYGAGLKAAARTNEPERFLDSLLVTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
LIEARSHERLGLLATHCPEPELAKFYKGLMASEARHFGTYWVLADTYFDREIVSQRLDEL
HHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHH
AIVESELLENLHPEPRIHS
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]