Click here to switch to the map view.

The map label for this gene is gacS [H]

Identifier: 17230389

GI number: 17230389

Start: 3535277

End: 3537217

Strand: Reverse

Name: gacS [H]

Synonym: all2897

Alternate gene names: 17230389

Gene position: 3537217-3535277 (Counterclockwise)

Preceding gene: 17230390

Following gene: 17230386

Centisome position: 55.15

GC content: 41.83

Gene sequence:

>1941_bases
ATGGCACAACCGCTAACCGTTCTAATTATTGATGATTGTGTTGAAGATCGCCAAGTTTATCGCCGCTATCTCCAGCAAGA
CCAAGAGCATAACTATACAATTCTGGAAAAAGACTCAGGAGAGGAAGCACTGGAATTATGTCGGCAGTTGCAACCTGATG
TAATTTTATTAGACTTTTTACTGCCAGACCAAGATGGACTAGAATTTCTGGCTGAATTACAAAAGCTGGTCAAGGGAAGT
ATGCCAGCTATAGTCATGTTAACTGGTTACGGTAATGAAGCGATCGCCGTACAAGCAATGAAAAGTGGTGTTCATGACTA
TCTGGTAAAAGAACAAACAACAGCAGAACGTTTACGCTCAACTCTCCACTCAGCAATTATCCAAACGCGATTACGTCAAG
AACTCTCCCGGAGTGAGGAGCAATTACACAATAGCCAAAAATTTATTAAACGCATTGCAGAAACCACTCCGGGAATTCTA
TACGTTTACGATTTAGTCGAAAAGCGCAATGTTTATCTCAATGGTAAGGTTGGAGATTTATTAGGTTATACTCCCCAAAC
GATTCAAGATTTGGGAAAAGAATTTCTCATCAAATTAATGCACCCTGAGGATTTAGTTCAACTTCCGCAAGTATTTCAGC
AATTTGACTCAGCCAAAGATGGGGATATCATCGAACATGAGTATAGGATGCACCACGCTAATGGTGGATGGTGTTGGTTT
TTTAGTCGTAATTCCGTGTTCACGAGGAATGCTGATGGTTCACCACGCCAAATAGTTGGTACAGCCTTTGATATCACTGC
TCGTAAGCAAGTTGAAGAAGAACTGCGCTCTAGCAATGAACGTTTCCGACTAGCAGCCGCCGCCATTAATGCTCTCATTT
ATGAATGGGATGTAAAAAAAGGTACGGTGACCAGAACGGAGGGACTGACGCGGATTTTAGGCTACTCTGTGGAGGAAGCC
ACCCCAACTATTGAATGGTGGCAAGAGCAAATTCATCCTGAAGACCAGGATTTTTTGGTTCAGCAATTCCAAAATTTACC
TGTTAATCAAAATCGTTATACTTTTGAATATCGCATTCGACACAAAAACAAACAGTATTTATACGTATTAGACCAAGGGG
TAATCACCAGAGATGAAAATGGCCAGCCAGTGCGAGTAGTCGGCAGTACAACCGATATTAGCGATCGCAAACGTGCGGAA
ACAGAACGCAACCAACTATTGCAATTAGAAAAATTAGCTCGTGCAGAAGCGGAAGCCGCCAATCACACAAAAGACGAGTT
TGTGGCCATGGTATCTCATGATTTGCGATCGCCTCTGAATGCAATTCTCGGTTGGTCACAATTGCTACGTACTCGCCAGC
TTGATGAAACCACATTTACCCGTGCCTTAGAAACCATTGAACGCAATGCTCAATCACAGTCAAAACTTTTGGAAGACTTA
CTGGATATGTCTCGTGTCCTCAGAGGTAAGTTACAGTTGGAATTTTGCCAAGTAGATTTAGCTGCCATTATTGGTGTAGC
AATTGAGACTGCTTATCCCTCAGCAAAAGCTAAGAATATTCATTTAAAGTCTGTGATAGATGAATCAATTCCACTGATTC
CTGGTGATATCAATCGCTTGTTACAAGTTTTAGGCAATCTGCTCTCAAATGCGATTAAGTTCACACCCCCAGAGGGACAG
ATCACAGTCCAACTATCATATACAGACTCTGAAGCCCATATCACGGTTACTGATACAGGTATTGGTATTAAGTCAGAATT
TTTACCCTACGTTTTTGATCGCTACCGTCAAGCAGACTGCCAACATAAACAACATGGTTTGGGTTTAGGGTTAGCGATCG
CCCGTTATCTCATAGAATTACACGGTGGGGCGATTCATGTCACTAGCCCTGGTGAAGGACAAGGAACTACTTTCACTATT
AAATTACTTTTGTATAAATAA

Upstream 100 bases:

>100_bases
TTAAGCCTATAGATATAAACCGTTTAGTTAAAACAATTCACAGTTTTTTAACTTACTGGTTAGATATTGTGATTCTTCCT
GATGCTGTTAGCAATTAGTC

Downstream 100 bases:

>100_bases
TATTTGTGCAAATTGGTAATGCGTAAAGATTCCCTACTCTCCATCTGATGCGGTGATTGGTGATTTTTTATTTGCCAATC
CCGAATTCCTCACAAGTTCC

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MAQPLTVLIIDDCVEDRQVYRRYLQQDQEHNYTILEKDSGEEALELCRQLQPDVILLDFLLPDQDGLEFLAELQKLVKGS
MPAIVMLTGYGNEAIAVQAMKSGVHDYLVKEQTTAERLRSTLHSAIIQTRLRQELSRSEEQLHNSQKFIKRIAETTPGIL
YVYDLVEKRNVYLNGKVGDLLGYTPQTIQDLGKEFLIKLMHPEDLVQLPQVFQQFDSAKDGDIIEHEYRMHHANGGWCWF
FSRNSVFTRNADGSPRQIVGTAFDITARKQVEEELRSSNERFRLAAAAINALIYEWDVKKGTVTRTEGLTRILGYSVEEA
TPTIEWWQEQIHPEDQDFLVQQFQNLPVNQNRYTFEYRIRHKNKQYLYVLDQGVITRDENGQPVRVVGSTTDISDRKRAE
TERNQLLQLEKLARAEAEAANHTKDEFVAMVSHDLRSPLNAILGWSQLLRTRQLDETTFTRALETIERNAQSQSKLLEDL
LDMSRVLRGKLQLEFCQVDLAAIIGVAIETAYPSAKAKNIHLKSVIDESIPLIPGDINRLLQVLGNLLSNAIKFTPPEGQ
ITVQLSYTDSEAHITVTDTGIGIKSEFLPYVFDRYRQADCQHKQHGLGLGLAIARYLIELHGGAIHVTSPGEGQGTTFTI
KLLLYK

Sequences:

>Translated_646_residues
MAQPLTVLIIDDCVEDRQVYRRYLQQDQEHNYTILEKDSGEEALELCRQLQPDVILLDFLLPDQDGLEFLAELQKLVKGS
MPAIVMLTGYGNEAIAVQAMKSGVHDYLVKEQTTAERLRSTLHSAIIQTRLRQELSRSEEQLHNSQKFIKRIAETTPGIL
YVYDLVEKRNVYLNGKVGDLLGYTPQTIQDLGKEFLIKLMHPEDLVQLPQVFQQFDSAKDGDIIEHEYRMHHANGGWCWF
FSRNSVFTRNADGSPRQIVGTAFDITARKQVEEELRSSNERFRLAAAAINALIYEWDVKKGTVTRTEGLTRILGYSVEEA
TPTIEWWQEQIHPEDQDFLVQQFQNLPVNQNRYTFEYRIRHKNKQYLYVLDQGVITRDENGQPVRVVGSTTDISDRKRAE
TERNQLLQLEKLARAEAEAANHTKDEFVAMVSHDLRSPLNAILGWSQLLRTRQLDETTFTRALETIERNAQSQSKLLEDL
LDMSRVLRGKLQLEFCQVDLAAIIGVAIETAYPSAKAKNIHLKSVIDESIPLIPGDINRLLQVLGNLLSNAIKFTPPEGQ
ITVQLSYTDSEAHITVTDTGIGIKSEFLPYVFDRYRQADCQHKQHGLGLGLAIARYLIELHGGAIHVTSPGEGQGTTFTI
KLLLYK
>Mature_645_residues
AQPLTVLIIDDCVEDRQVYRRYLQQDQEHNYTILEKDSGEEALELCRQLQPDVILLDFLLPDQDGLEFLAELQKLVKGSM
PAIVMLTGYGNEAIAVQAMKSGVHDYLVKEQTTAERLRSTLHSAIIQTRLRQELSRSEEQLHNSQKFIKRIAETTPGILY
VYDLVEKRNVYLNGKVGDLLGYTPQTIQDLGKEFLIKLMHPEDLVQLPQVFQQFDSAKDGDIIEHEYRMHHANGGWCWFF
SRNSVFTRNADGSPRQIVGTAFDITARKQVEEELRSSNERFRLAAAAINALIYEWDVKKGTVTRTEGLTRILGYSVEEAT
PTIEWWQEQIHPEDQDFLVQQFQNLPVNQNRYTFEYRIRHKNKQYLYVLDQGVITRDENGQPVRVVGSTTDISDRKRAET
ERNQLLQLEKLARAEAEAANHTKDEFVAMVSHDLRSPLNAILGWSQLLRTRQLDETTFTRALETIERNAQSQSKLLEDLL
DMSRVLRGKLQLEFCQVDLAAIIGVAIETAYPSAKAKNIHLKSVIDESIPLIPGDINRLLQVLGNLLSNAIKFTPPEGQI
TVQLSYTDSEAHITVTDTGIGIKSEFLPYVFDRYRQADCQHKQHGLGLGLAIARYLIELHGGAIHVTSPGEGQGTTFTIK
LLLYK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=272, Percent_Identity=32.7205882352941, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=33.7209302325581, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=35.4166666666667, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788713, Length=250, Percent_Identity=32, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=31.9327731092437, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081816, Length=257, Percent_Identity=33.852140077821, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788549, Length=262, Percent_Identity=33.206106870229, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=29.2682926829268, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=29.646017699115, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=27.8538812785388, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=252, Percent_Identity=25.7936507936508, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=244, Percent_Identity=29.9180327868852, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082128, Length=270, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=203, Percent_Identity=26.6009852216749, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790300, Length=260, Percent_Identity=27.3076923076923, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=155, Percent_Identity=32.9032258064516, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73601; Mature: 73469

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPLTVLIIDDCVEDRQVYRRYLQQDQEHNYTILEKDSGEEALELCRQLQPDVILLDFL
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEE
LPDQDGLEFLAELQKLVKGSMPAIVMLTGYGNEAIAVQAMKSGVHDYLVKEQTTAERLRS
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHCCHHHHHHCCHHHHHHHHH
TLHSAIIQTRLRQELSRSEEQLHNSQKFIKRIAETTPGILYVYDLVEKRNVYLNGKVGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCEEECCCHHHH
LGYTPQTIQDLGKEFLIKLMHPEDLVQLPQVFQQFDSAKDGDIIEHEYRMHHANGGWCWF
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHEEECCCCEEEE
FSRNSVFTRNADGSPRQIVGTAFDITARKQVEEELRSSNERFRLAAAAINALIYEWDVKK
EECCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCC
GTVTRTEGLTRILGYSVEEATPTIEWWQEQIHPEDQDFLVQQFQNLPVNQNRYTFEYRIR
CCEEHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEE
HKNKQYLYVLDQGVITRDENGQPVRVVGSTTDISDRKRAETERNQLLQLEKLARAEAEAA
ECCCCEEEEEECCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NHTKDEFVAMVSHDLRSPLNAILGWSQLLRTRQLDETTFTRALETIERNAQSQSKLLEDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDMSRVLRGKLQLEFCQVDLAAIIGVAIETAYPSAKAKNIHLKSVIDESIPLIPGDINRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCCHHHH
LQVLGNLLSNAIKFTPPEGQITVQLSYTDSEAHITVTDTGIGIKSEFLPYVFDRYRQADC
HHHHHHHHHCCEECCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
QHKQHGLGLGLAIARYLIELHGGAIHVTSPGEGQGTTFTIKLLLYK
HHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEC
>Mature Secondary Structure 
AQPLTVLIIDDCVEDRQVYRRYLQQDQEHNYTILEKDSGEEALELCRQLQPDVILLDFL
CCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEE
LPDQDGLEFLAELQKLVKGSMPAIVMLTGYGNEAIAVQAMKSGVHDYLVKEQTTAERLRS
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHCCHHHHHHCCHHHHHHHHH
TLHSAIIQTRLRQELSRSEEQLHNSQKFIKRIAETTPGILYVYDLVEKRNVYLNGKVGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCEEECCCHHHH
LGYTPQTIQDLGKEFLIKLMHPEDLVQLPQVFQQFDSAKDGDIIEHEYRMHHANGGWCWF
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHEEECCCCEEEE
FSRNSVFTRNADGSPRQIVGTAFDITARKQVEEELRSSNERFRLAAAAINALIYEWDVKK
EECCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCC
GTVTRTEGLTRILGYSVEEATPTIEWWQEQIHPEDQDFLVQQFQNLPVNQNRYTFEYRIR
CCEEHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEE
HKNKQYLYVLDQGVITRDENGQPVRVVGSTTDISDRKRAETERNQLLQLEKLARAEAEAA
ECCCCEEEEEECCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NHTKDEFVAMVSHDLRSPLNAILGWSQLLRTRQLDETTFTRALETIERNAQSQSKLLEDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDMSRVLRGKLQLEFCQVDLAAIIGVAIETAYPSAKAKNIHLKSVIDESIPLIPGDINRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCCHHHH
LQVLGNLLSNAIKFTPPEGQITVQLSYTDSEAHITVTDTGIGIKSEFLPYVFDRYRQADC
HHHHHHHHHCCEECCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
QHKQHGLGLGLAIARYLIELHGGAIHVTSPGEGQGTTFTIKLLLYK
HHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]