| Definition | Nostoc sp. PCC 7120, complete genome. |
|---|---|
| Accession | NC_003272 |
| Length | 6,413,771 |
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The map label for this gene is phoA [H]
Identifier: 17230335
GI number: 17230335
Start: 3463675
End: 3465558
Strand: Reverse
Name: phoA [H]
Synonym: all2843
Alternate gene names: 17230335
Gene position: 3465558-3463675 (Counterclockwise)
Preceding gene: 17230336
Following gene: 17230334
Centisome position: 54.03
GC content: 46.55
Gene sequence:
>1884_bases GTGTTTGGCACACCAGGCGTTGAATCTGCCCTAGCGGCTGGTAATGGAATTAACGTCATCATCATGATTGGTGATGGGAT GGGTTGGGAAATGGCCAGAGCCGCAGCCGTAGCTAAAGGCGCTCCCTTCTATACCAGTGGGAAAGGTTCTGGTCTGAGCT TTCAAAAGCTAACTGGATATGGAATTGTGACAACCTACGGGACAACAGTCCAAGGTAGTACATCAACTAATCCAGATAAT GAGACACGTTCAGCCAATAACTCGGCTCTAGATGGAAGCAATCCATTAACTGGCGCAAGTCCTGTGCGTCCCAACTTCTC ATTCTCACCTGCTCCTTTTAACCCAGGCGATCGCGCTGATGGTAACAGTCCAGCACCCGGTAACTTGGCTGGTTATGACC CCAGCAAAGGCGGCCCTGTACCTTGGATACCTCTATCTCCCGCTAATCCTGGTAACTACGATAAAGAGTACATCAAATAT AGCTATCCCGACTCTGCAAACACCGCTACAACTCTATACACAGGTGTTAAGAGCTATAACAACGCAATGGGGGTAGATAT TTACGAGCAGAAGTTAAAAACTATCCTAGAAATTGCTAAAGAAGAGGGCAAAGCTACAGGGCTAGTAACTTCTGTACCCA TCACCCACGCTACACCAGGGGCTGCGGCTTCTTACGTCAACCGTCGTAGCAAGTACGACAGTGTTTATGACCCTACCAAG ACCAACCAAGACAGCATCTTACAACAGATGTTGCTAGACTTTCAGCCCAACATATTGTTAGGTGGTGGTCATCCTCTCGA TATGCAAAATAGAGGCGCGACAGGCCCAGTTAATAACTACACCTTCATCACCCAAGACACTTACGAACATCTCAAAAATA ACCCCACTCCCACATCTAACCGTTATGGTTATACCTTCTTGGAGCGTGGGCCTAATGCTACTCAAACATTACTAAACACA GCAGCTCAAATTGATCCCAATAGAGGCGATAAACTCTTGGGTCTATACGGCGCTCGTGGACAAAACGGTAATATTCCTCT AAGTTCTTCCAAGGGAGACTACAGCACCACAGGTTTAGATAACTTCTCTGTCTATAGCACTGTTGTGAGAGGTGGAACCC CCGCTACTTGCCCATCAGGACAAATCATTCCTGGCTCTATCAGTGAGTGTGTACCCGTGCTGGATGGCAATGGTAATCCT GTGACTGGCCCCACACCTGATAGAGTCCGTCCCCTCGCTCCCGGTGAAACCGATGCTAGTTTCATCGCCAGAGAAATTAA CGAAAACCCTACCTTGGCTGATTTAAGCAAGGCTGCTCTCAATGTCTTAGGTAAAGACAAAGATGGCTTTTGGTTAATGA TTGAGGGTGGTGATATTGACTGGTCTGCTCATGATGACAACATGGATAACCTGATTGGAACCATGAATGACTTTGATAAA GCAGTCCAAGAGGTGATCAGTTGGATTAACAAAAATGGTGGTTGGAGTAAGAACCTGTTAATCGTGACAGCTGACCATGA CCATTATTTGACTCTAAATAATGACTTTGTTTCTAAGCTGACTCCTAATCCCCACCCCAATCAATCCAGAGGCTTGAAGT ATAACGCCAAAGAAATTACTTACAACAAGCACAACCCTCAAGATGCGGGACACTTCTGGGGTTCTGATCCCACTCAGAAA TACCTCTGGGGAAGTCACAGCCGAAGACCTGTCCCTGTTTATTTCCAAGGTGCTTATTCTTCCAACCTCGTGAGATATTT TGGTCAACCGGTGCAGTTCACAGATAGTTCTGGTTCCTACACTGCATCTGGTATTCGCAGTGTTGTTGATCAAAGCCACA TCTTCCAAGCCATGAAAGCTGCATTAACTCAGCCACCACTCTAG
Upstream 100 bases:
>100_bases TTAATTAACTGCAAGGCGCACTGCAATGATTTCACTTTTGGAAAGATGTCGGCGAGCGTTCAGCTTTGCTATGGCTTGTT TTTTCTGCACTATGGGGCTA
Downstream 100 bases:
>100_bases TGGAGAGTTTACATAGCTCATATATATTCTGTAAATCAAGGAAAAAAATGACTAGCTCTATCCTCAAAAAAGTAGCAGGT GTAACAGTAGGAACTACTTT
Product: alkaline phosphatase
Products: NA
Alternate protein names: High molecular weight phosphatase; H-AP [H]
Number of amino acids: Translated: 627; Mature: 627
Protein sequence:
>627_residues MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL
Sequences:
>Translated_627_residues MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL >Mature_627_residues MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGYGIVTTYGTTVQGSTSTNPDN ETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRADGNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKY SYPDSANTATTLYTGVKSYNNAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSNRYGYTFLERGPNATQTLLNT AAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLDNFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNP VTGPTPDRVRPLAPGETDASFIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEITYNKHNPQDAGHFWGSDPTQK YLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSYTASGIRSVVDQSHIFQAMKAALTQPPL
Specific function: Has only a phosphomonoesterase activity [H]
COG id: COG1785
COG function: function code P; Alkaline phosphatase
Gene ontology:
Cell location: Secreted. Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the alkaline phosphatase family [H]
Homologues:
Organism=Escherichia coli, GI48994877, Length=112, Percent_Identity=41.0714285714286, Blast_Score=71, Evalue=2e-13,
Paralogues:
None
Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR017849 - InterPro: IPR001952 - InterPro: IPR018299 - InterPro: IPR017850 [H]
Pfam domain/function: PF00245 Alk_phosphatase [H]
EC number: =3.1.3.1 [H]
Molecular weight: Translated: 67221; Mature: 67221
Theoretical pI: Translated: 6.14; Mature: 6.14
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGY CCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHCCCC GIVTTYGTTVQGSTSTNPDNETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRAD EEEEECCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC GNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKYSYPDSANTATTLYTGVKSYN CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHC NAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSN CCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCC RYGYTFLERGPNATQTLLNTAAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLD CCCEEHEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC NFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNPVTGPTPDRVRPLAPGETDAS CHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHH FIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEIT HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCEECCCEEE YNKHNPQDAGHFWGSDPTQKYLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSY ECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCE TASGIRSVVDQSHIFQAMKAALTQPPL EHHHHHHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MFGTPGVESALAAGNGINVIIMIGDGMGWEMARAAAVAKGAPFYTSGKGSGLSFQKLTGY CCCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEECCCCCCCCHHHCCCC GIVTTYGTTVQGSTSTNPDNETRSANNSALDGSNPLTGASPVRPNFSFSPAPFNPGDRAD EEEEECCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC GNSPAPGNLAGYDPSKGGPVPWIPLSPANPGNYDKEYIKYSYPDSANTATTLYTGVKSYN CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHC NAMGVDIYEQKLKTILEIAKEEGKATGLVTSVPITHATPGAAASYVNRRSKYDSVYDPTK CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCC TNQDSILQQMLLDFQPNILLGGGHPLDMQNRGATGPVNNYTFITQDTYEHLKNNPTPTSN CCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCC RYGYTFLERGPNATQTLLNTAAQIDPNRGDKLLGLYGARGQNGNIPLSSSKGDYSTTGLD CCCEEHEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC NFSVYSTVVRGGTPATCPSGQIIPGSISECVPVLDGNGNPVTGPTPDRVRPLAPGETDAS CHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHH FIAREINENPTLADLSKAALNVLGKDKDGFWLMIEGGDIDWSAHDDNMDNLIGTMNDFDK HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH AVQEVISWINKNGGWSKNLLIVTADHDHYLTLNNDFVSKLTPNPHPNQSRGLKYNAKEIT HHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCEECCCEEE YNKHNPQDAGHFWGSDPTQKYLWGSHSRRPVPVYFQGAYSSNLVRYFGQPVQFTDSSGSY ECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCE TASGIRSVVDQSHIFQAMKAALTQPPL EHHHHHHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043; 8454193 [H]