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The map label for this gene is mycA [H]

Identifier: 17230130

GI number: 17230130

Start: 3154511

End: 3155746

Strand: Reverse

Name: mycA [H]

Synonym: all2638

Alternate gene names: 17230130

Gene position: 3155746-3154511 (Counterclockwise)

Preceding gene: 17230131

Following gene: 17230129

Centisome position: 49.2

GC content: 46.52

Gene sequence:

>1236_bases
ATGGGACTAGGAATTAACTTATTTGGACATAACCCCCCATTCATTCAAACAGCCATCAGCGAACAACTACAACAGGGAAT
GCACATCGGTGTCCAAAATACACTAGCTGGCGAAGTAGCCCAACTCATCAGCGAACTAACAGGTGCAGAAAGAGTCACCT
TTAGTAACACAGGTACAGAAGCCGTCATGACAGCCGTGCGTATCGCCCGCGCCGCCACAAAACGCCCAAAAATAGCGATT
TTCACCAACTCCTACCACGGACACTTTGATTCTGTACTTGTCCGGGCAACAATGACAGAATATGCTAAAAAAGCCGCCCG
TCGCATCATCGCCGCTAAATCTGGTAGTTTTATCGGTAGACTAGCCCAACCAATTCAAGCCCTTTTCACAGCCAACCTCA
ATCCCAAAGCCGTACCCGCAGCCCCAGGTATTCCCAACAGTGCAGCTGCTGACGTACTGATTTTAGAGTACGGTAACGAA
CATTCTTTAAAGCTGATTCGCAAACATCGCCGAGAACTCGCCGCCGTCTTAGTAGAACCAGTACAAAGCCGTTGTCCCGA
ACTGCAACCGCGTGAATTTCTGCAACAACTACGACAAATCACCCAAGAACTAGGAATAGTCCTGATTTTTGACGAAATGG
TGACAGGTTTTCGCATCCACCCAGGAGGAGCGCAAGCACACTTTGGTGTAAAAGCCGACATTGCAACCTATAGCAAAATC
GTTGGGGGAGGTCTACCGCTTTCGGTAATTGCTGGGAAAGCTAGTTACATGAACTACATAGACGGTGGACAATGGCAATT
TGGAGATGATTCCACGCCTCAAGTTCCCACAACCTTCTTTGCTGGAACATTTTGCAAACATCCCCTCGCCCTCGCCGCCG
CCCGTGCCACCTTACAACACCTCAAAATTTCAGGTATTGAATTACACAACGAATTAAATCAACGGACTACGCAACTCGTC
AACGATTTAAATACTCACATGACACAGCAGGGTATAGCTATCAAGTTTACTAGCTTTGGCTCATTTTTTGCTGTAGCAGC
CTCCCAAAGCGCAGTTCCCCCAATGGCGATAAACTTACTCTCTTATCACCTCATCACAAAAGGAATACATCTGCGCCAAG
GAGACAAAGGCGGTTTTCTTTCCACAGCACACACCGAAGAAGATATTAATCTCATCAAAAAAGCCTTTATCGACAGTATT
AACGAACTACGCAACGAAGGATTTATTAACAACTAA

Upstream 100 bases:

>100_bases
TGCACCAGAAACCAAAGAACTCTGCTACCCAATTGCTATCGAAAAATCCAACGGTTCTCGCCTGTGGGATGTCGATGGAA
ACGAATACATAGACATCCTC

Downstream 100 bases:

>100_bases
TCTTCTCTCCGCGCCCCTCCGCGTTTCCCTCTGCGCCCCTTTGCGTTAAAAAAATATGCTCCTAGAACTCTTAAACCCCG
CAGAACTCCCCATCCAACAA

Product: glutamate-1-semialdehyde aminotransferase

Products: 5-Amino-4-oxopentanoate [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MGLGINLFGHNPPFIQTAISEQLQQGMHIGVQNTLAGEVAQLISELTGAERVTFSNTGTEAVMTAVRIARAATKRPKIAI
FTNSYHGHFDSVLVRATMTEYAKKAARRIIAAKSGSFIGRLAQPIQALFTANLNPKAVPAAPGIPNSAAADVLILEYGNE
HSLKLIRKHRRELAAVLVEPVQSRCPELQPREFLQQLRQITQELGIVLIFDEMVTGFRIHPGGAQAHFGVKADIATYSKI
VGGGLPLSVIAGKASYMNYIDGGQWQFGDDSTPQVPTTFFAGTFCKHPLALAAARATLQHLKISGIELHNELNQRTTQLV
NDLNTHMTQQGIAIKFTSFGSFFAVAASQSAVPPMAINLLSYHLITKGIHLRQGDKGGFLSTAHTEEDINLIKKAFIDSI
NELRNEGFINN

Sequences:

>Translated_411_residues
MGLGINLFGHNPPFIQTAISEQLQQGMHIGVQNTLAGEVAQLISELTGAERVTFSNTGTEAVMTAVRIARAATKRPKIAI
FTNSYHGHFDSVLVRATMTEYAKKAARRIIAAKSGSFIGRLAQPIQALFTANLNPKAVPAAPGIPNSAAADVLILEYGNE
HSLKLIRKHRRELAAVLVEPVQSRCPELQPREFLQQLRQITQELGIVLIFDEMVTGFRIHPGGAQAHFGVKADIATYSKI
VGGGLPLSVIAGKASYMNYIDGGQWQFGDDSTPQVPTTFFAGTFCKHPLALAAARATLQHLKISGIELHNELNQRTTQLV
NDLNTHMTQQGIAIKFTSFGSFFAVAASQSAVPPMAINLLSYHLITKGIHLRQGDKGGFLSTAHTEEDINLIKKAFIDSI
NELRNEGFINN
>Mature_410_residues
GLGINLFGHNPPFIQTAISEQLQQGMHIGVQNTLAGEVAQLISELTGAERVTFSNTGTEAVMTAVRIARAATKRPKIAIF
TNSYHGHFDSVLVRATMTEYAKKAARRIIAAKSGSFIGRLAQPIQALFTANLNPKAVPAAPGIPNSAAADVLILEYGNEH
SLKLIRKHRRELAAVLVEPVQSRCPELQPREFLQQLRQITQELGIVLIFDEMVTGFRIHPGGAQAHFGVKADIATYSKIV
GGGLPLSVIAGKASYMNYIDGGQWQFGDDSTPQVPTTFFAGTFCKHPLALAAARATLQHLKISGIELHNELNQRTTQLVN
DLNTHMTQQGIAIKFTSFGSFFAVAASQSAVPPMAINLLSYHLITKGIHLRQGDKGGFLSTAHTEEDINLIKKAFIDSIN
ELRNEGFINN

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=401, Percent_Identity=31.6708229426434, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787560, Length=266, Percent_Identity=30.0751879699248, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693181, Length=312, Percent_Identity=27.2435897435897, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 5.4.3.8 [C]

Molecular weight: Translated: 44624; Mature: 44493

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLGINLFGHNPPFIQTAISEQLQQGMHIGVQNTLAGEVAQLISELTGAERVTFSNTGTE
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
AVMTAVRIARAATKRPKIAIFTNSYHGHFDSVLVRATMTEYAKKAARRIIAAKSGSFIGR
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAQPIQALFTANLNPKAVPAAPGIPNSAAADVLILEYGNEHSLKLIRKHRRELAAVLVEP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
VQSRCPELQPREFLQQLRQITQELGIVLIFDEMVTGFRIHPGGAQAHFGVKADIATYSKI
HHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHCCEEECCCCCCCCCCCCHHHHHHHHH
VGGGLPLSVIAGKASYMNYIDGGQWQFGDDSTPQVPTTFFAGTFCKHPLALAAARATLQH
HCCCCCHHHHHCCHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
LKISGIELHNELNQRTTQLVNDLNTHMTQQGIAIKFTSFGSFFAVAASQSAVPPMAINLL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCHHHHHHH
SYHLITKGIHLRQGDKGGFLSTAHTEEDINLIKKAFIDSINELRNEGFINN
HHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GLGINLFGHNPPFIQTAISEQLQQGMHIGVQNTLAGEVAQLISELTGAERVTFSNTGTE
CCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
AVMTAVRIARAATKRPKIAIFTNSYHGHFDSVLVRATMTEYAKKAARRIIAAKSGSFIGR
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAQPIQALFTANLNPKAVPAAPGIPNSAAADVLILEYGNEHSLKLIRKHRRELAAVLVEP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
VQSRCPELQPREFLQQLRQITQELGIVLIFDEMVTGFRIHPGGAQAHFGVKADIATYSKI
HHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHCCEEECCCCCCCCCCCCHHHHHHHHH
VGGGLPLSVIAGKASYMNYIDGGQWQFGDDSTPQVPTTFFAGTFCKHPLALAAARATLQH
HCCCCCHHHHHCCHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
LKISGIELHNELNQRTTQLVNDLNTHMTQQGIAIKFTSFGSFFAVAASQSAVPPMAINLL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCHHHHHHH
SYHLITKGIHLRQGDKGGFLSTAHTEEDINLIKKAFIDSINELRNEGFINN
HHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Ethanolamine; Methylamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Glutamate 1-semialdehyde [C]

Specific reaction: L-Glutamate 1-semialdehyde --> 5-Amino-4-oxopentanoate [C]

General reaction: Group transfer (intramolecular amino group isomerization) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]