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The map label for this gene is topA [H]

Identifier: 17230123

GI number: 17230123

Start: 3143069

End: 3145252

Strand: Reverse

Name: topA [H]

Synonym: all2631

Alternate gene names: 17230123

Gene position: 3145252-3143069 (Counterclockwise)

Preceding gene: 17230124

Following gene: 17230122

Centisome position: 49.04

GC content: 46.43

Gene sequence:

>2184_bases
ATGCCGAAACGCCTGCTAGTGGTTGAATCTCCGGGTAAAGTGAAAAAGCTCAGTCAAATTTTGGGTTCTGAGTGGATTGT
CCGTGCTTCTTGTGGACATATTCGGGAACTGAGTGATGAGGGTGAGGATGCTTTGGGGTTCAGCATGGATGGTAGTAGTG
TGCAGTGTCACTACGTACCGCGTGACCAACGTTCAAAAGAAACTATCCAGCAGTTAAAGAGTGCGGCGAAGCAGGTAAGT
GAAGTTGTGTTAGGGACTGACCCGGATAGGGAAGGAGAAACCATTGCTTGGCATCTCAAGGAAGTTTTGGGATTGAGAGA
ACCGAAACGGGTTGTCTATACAGAGATTACAGCCTCGGCGGTACAGAGTGCGATCGCTCATCCGCGCAAACTGGACTTAA
ATTTAGTCGGGGCGGGATTGTGTCGAGATTGTCTAGATAAGTTGGTCGGGTATAAGGGTAGTCCTCTGGTTTGGGCGTTA
AATAATGGGGCCAAGAGTGTGGGGAGAGTGCAGAGTTCTACACTCCACCTGATTTGTCAACGGGAGAGGGAAATTCAAAG
TTTTGTACCTCAAGATTATTGGAGTGTTTGGGTTGATTATGTTGAAGGTTTCCGCGCTTTCTATAAAGGTGCAGTTAACA
CTCGCTCAAACTCTCCAGAAGCAGAAGTAGAAATTCATGATGATGCGGCGGGAAATAGCACAACTGCGCCTGAGTCTACC
CGCGTACTTGCAGAAGCCGAAGCTAACCGTTTAGTAGAAGAAGCACGCCGTCATCCCCACCAAATCGTGCAGTATGAGGG
AAAAGTTGCCAACCGTCAACCACCGCCACCTTTTATTACTTCCACTCTGCAACAAGCGGCTGGTTCTAAGTTGAAGTTTG
CACCAGAGAAGACGATGCAGCTAGCCCAAAAGTTGTATGAGGCTGGGTTGATTACATATATGCGGACAGACTCGGTAATG
TTGAGTCCAGAGTTTTGTGAAAGTGCGCGTCAGTGGTTGGAACAAAATGACCCGCAGAATGTACCGACACAGGTTGCCAG
ACATCGCAGTAGCAAGACAGCCCAACAAGGACACGAAGCAATTCGCCCCACTGATGTGTTTCGTCCTTCCGCCCAGTTGC
GAATAGAACTATCCACGGATGAGTTTAACTTGTATGTGATGATTTGGAAACGGGCGATCGCTTCCCAATGTCGTCCAGCC
CAACTACGCAAAACGGTCGTAATCACCCAGTCTGGTCAAATTCTTTGGCAAGCAAGAGGACAAGTAGTAGAATTTCTCGG
TTATACCCGCTACTGGCCTAATCTCAGCAAGGATACCCTCTTACCCACCTTGCAACAAGGGCAAATGGTAACTTTAGAAA
ATGCCGGTCACGAGAAAAAACAAACCCAACCTCCACCACGCTACAGCGAACCAAAGTTAGTGCAACTTATGGAACGTAAA
GGCATTGGTCGTCCCAGTACCTATTCTCCCACCATCGCCACCCTGAAGAAACGGGGTTACGTCGAACTGACCAAAGACAA
CTTACACCCAACAAATCTAGGTTTAGAAGTTGATACTTTCTTACAAAAAGCCCTACCGGATTTACTAGAAGCAGAATTTA
CTGCCAAAATGGAAAATGCCCTCGATGCGATCGCTGAAGGTAAACAACCTTGGCAGATATACTTGACCACTTGGAATCAG
AATTATTTTGCCCCAGCTTTAGCCAAAGCTAAGACTGTCACTGTCGATTCAGGCAAGACGACAAAAACTTTTCCCGCGCG
TCAATACGATACCAGCCGGACTCGCTGCCCTGATTGCAATAACTTTCTCAGCAAAATTCCCAGCAGTAAACTGAAGAAAA
AATACTTCCTCAAATGCACTAGCGGTTGTGAAAACACTGTCTTATTTTGGAGTGAATTTAGTAAGACTTGGCAAGCGCCA
CGAACAAAAGATGACAAGATGGCAGAAAATGGGGAAAAGAATCATCTCTCACCTTCAACAAAACTTCCCACTCAGTTGAC
AGCATACCCCTGTCCAGTATGTAAAAAACCTTTGGAAGAGTATACTTACACCAAAGATGGACAGAAAAAAACTATGCTGC
GTTGCTCTGCATCCTCATCTCGCACTGACAAAAAGCATAAAGATGTGGCTTATTTCCACACTGCAAAAGGTTGGTGGAGT
CCTAAGTTTGGGGAAATAACCTAG

Upstream 100 bases:

>100_bases
AATTAGCTTTAAGGATGGGGGTAATGGTATCGCTATATGGGACGCGAGAGTAGAGCAATTTGATTTTTGCTGTTCCCTTT
TTCTTACTTTTGAATTGTTT

Downstream 100 bases:

>100_bases
CCATGCAACGTTTTCATCCCCCCTAGCCCCCTTAAAAAAAGCAGGGCTGTTTCATTATCTCAGACTTTCCAAGACGAATT
TGGAATTTTAAATCAATCTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGSSVQCHYVPRDQRSKETIQQLKSAAKQVS
EVVLGTDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWAL
NNGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSNSPEAEVEIHDDAAGNSTTAPEST
RVLAEAEANRLVEEARRHPHQIVQYEGKVANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVM
LSPEFCESARQWLEQNDPQNVPTQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPA
QLRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQ
NYFAPALAKAKTVTVDSGKTTKTFPARQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAP
RTKDDKMAENGEKNHLSPSTKLPTQLTAYPCPVCKKPLEEYTYTKDGQKKTMLRCSASSSRTDKKHKDVAYFHTAKGWWS
PKFGEIT

Sequences:

>Translated_727_residues
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGSSVQCHYVPRDQRSKETIQQLKSAAKQVS
EVVLGTDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWAL
NNGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSNSPEAEVEIHDDAAGNSTTAPEST
RVLAEAEANRLVEEARRHPHQIVQYEGKVANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVM
LSPEFCESARQWLEQNDPQNVPTQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPA
QLRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQ
NYFAPALAKAKTVTVDSGKTTKTFPARQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAP
RTKDDKMAENGEKNHLSPSTKLPTQLTAYPCPVCKKPLEEYTYTKDGQKKTMLRCSASSSRTDKKHKDVAYFHTAKGWWS
PKFGEIT
>Mature_726_residues
PKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGSSVQCHYVPRDQRSKETIQQLKSAAKQVSE
VVLGTDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALN
NGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSNSPEAEVEIHDDAAGNSTTAPESTR
VLAEAEANRLVEEARRHPHQIVQYEGKVANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVML
SPEFCESARQWLEQNDPQNVPTQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQ
LRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERKG
IGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQN
YFAPALAKAKTVTVDSGKTTKTFPARQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPR
TKDDKMAENGEKNHLSPSTKLPTQLTAYPCPVCKKPLEEYTYTKDGQKKTMLRCSASSSRTDKKHKDVAYFHTAKGWWSP
KFGEIT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=605, Percent_Identity=24.4628099173554, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI4507635, Length=574, Percent_Identity=24.0418118466899, Blast_Score=116, Evalue=1e-25,
Organism=Escherichia coli, GI1787529, Length=652, Percent_Identity=34.0490797546012, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1788061, Length=544, Percent_Identity=25.7352941176471, Blast_Score=119, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=545, Percent_Identity=24.4036697247706, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=685, Percent_Identity=22.7737226277372, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640096, Length=574, Percent_Identity=23.5191637630662, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585251, Length=622, Percent_Identity=22.8295819935691, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81974; Mature: 81843

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGSSVQCHYVP
CCCCEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEECCCCEEEEECC
RDQRSKETIQQLKSAAKQVSEVVLGTDPDREGETIAWHLKEVLGLREPKRVVYTEITASA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHH
VQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALNNGAKSVGRVQSSTLHLICQ
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
REREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSNSPEAEVEIHDDAAGNSTTAPEST
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
RVLAEAEANRLVEEARRHPHQIVQYEGKVANRQPPPPFITSTLQQAAGSKLKFAPEKTMQ
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHH
LAQKLYEAGLITYMRTDSVMLSPEFCESARQWLEQNDPQNVPTQVARHRSSKTAQQGHEA
HHHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQLRKTVVITQSGQILWQARG
CCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHHHHCCCCHHHCEEEEEECCCCEEEHHHH
QVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
HHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHC
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENA
CCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDAIAEGKQPWQIYLTTWNQNYFAPALAKAKTVTVDSGKTTKTFPARQYDTSRTRCPDCN
HHHHHCCCCCEEEEEEEECCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
NFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPRTKDDKMAENGEKNHLSPST
HHHHHCCHHHHHHHHHEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
KLPTQLTAYPCPVCKKPLEEYTYTKDGQKKTMLRCSASSSRTDKKHKDVAYFHTAKGWWS
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCHHHCCEEEEEECCCCCC
PKFGEIT
CCCCCCC
>Mature Secondary Structure 
PKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGSSVQCHYVP
CCCEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEECCCCEEEEECC
RDQRSKETIQQLKSAAKQVSEVVLGTDPDREGETIAWHLKEVLGLREPKRVVYTEITASA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHH
VQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALNNGAKSVGRVQSSTLHLICQ
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
REREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSNSPEAEVEIHDDAAGNSTTAPEST
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
RVLAEAEANRLVEEARRHPHQIVQYEGKVANRQPPPPFITSTLQQAAGSKLKFAPEKTMQ
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHH
LAQKLYEAGLITYMRTDSVMLSPEFCESARQWLEQNDPQNVPTQVARHRSSKTAQQGHEA
HHHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQLRKTVVITQSGQILWQARG
CCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHHHHCCCCHHHCEEEEEECCCCEEEHHHH
QVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
HHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHC
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENA
CCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDAIAEGKQPWQIYLTTWNQNYFAPALAKAKTVTVDSGKTTKTFPARQYDTSRTRCPDCN
HHHHHCCCCCEEEEEEEECCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
NFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPRTKDDKMAENGEKNHLSPST
HHHHHCCHHHHHHHHHEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
KLPTQLTAYPCPVCKKPLEEYTYTKDGQKKTMLRCSASSSRTDKKHKDVAYFHTAKGWWS
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCHHHCCEEEEEECCCCCC
PKFGEIT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]