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The map label for this gene is ackA [H]

Identifier: 17230053

GI number: 17230053

Start: 3060033

End: 3061250

Strand: Reverse

Name: ackA [H]

Synonym: all2561

Alternate gene names: 17230053

Gene position: 3061250-3060033 (Counterclockwise)

Preceding gene: 17230055

Following gene: 17230049

Centisome position: 47.73

GC content: 46.63

Gene sequence:

>1218_bases
ATGAAAGTACTAATTTTGAATGCTGGTTCAAGCAGCCAAAAGAGTTGTCTATATGAAATTCCCGATGATGCTCTCCTGAG
TGAAGCACCCCAGCCGCTTTGGGAAGGGAAAGTTAACTGGACTCAAGACAGAAGTGTGGCGGAAATTGAGGTCAAAACAG
CTAGAGGTGAAACGCTCCATGAGTCTATATATGGTGATTCCCGTCAAGCACACGTCACCTATATGCTTTATACCCTCAGT
CGCGGTGCAACTAAGGTTATCGGTCAATTATCCGAAATCGATGTGGTGGGACATCGGGTGGTACATGGTGGGCAAAATTA
CCGCCATAGTGTAATTATTACTGAAGAAGTGAAACAGGCGATCGCTAAATTATCCAACCTCGCCCCAGCACATAATCCTG
CCGCCTTGGAAGGTATCGAAGCTATCGAAAAAAGTTTGGGTGATGTTCCCCAAGTAGCAGTGTTTGATACTGGCTTCCAT
GCCACTCTACCCGATGCCGCCGCTATTTATCCCGGCCCTTTTGAATGGGTGGAACAAGGTATCCGCCGCTATGGATTTCA
TGGCATTAGTCACCAATACTGTTCGGCTCGTGCGGCTCAAATCCTTGGTCGAGATTTGGCATCTCTGCGGATAATTACCT
GCCATCTGGGCAATGGTTGCTCTTTGGCAGCAATTAAAAATGGTCGCAGTATTGACACTACAATGGGTTTCACACCTTTA
GATGGGTTGATGATGGGTAGTCGTTCCGGTGCCATCGATCCGGGGATCATTGTTCACTTAATGCGACAATCAGATTACTC
TGCTGAAAGATTGGACTATGTTTTAAATAAAGCTTCCGGCTTACGCGGTATTTCTGGGGTTTCCAGCGATTTACCCCAGG
TCATAGAAGCCATTACCCAAGGTAACTACCGCGCCCAACTGGCGTGGGATATGTACGTACATCGGCTGCGTTCTGGGATT
GGTTCCATGTTGGCGAGTCTGGGGGGTTTGGATGTGTTGGTGTTTACCGCAGGCGTAGGGGAAAAATCAGCAGGTATTCG
CCAAGCAGCTTGTGAAGCCTTTGGGTTTTTAGGGTTAAAACTTGACCCAGAAAAGAACCAAAACAAACCAGTAGATATAG
ATATCGCCACTGCTGATTCTACAGTACGGGTGTTAGTAATTCATACTCAAGAAGATTGGGCGATCGCACAACAATGTTGG
CATTTGTTAAAAAGGTAA

Upstream 100 bases:

>100_bases
GTCTTTGCTTTTCCTAAAATATCGGTAATAAGTTTAATAAATACCTGAAATTAGGAAATGATATAGCTCACACTTCACAA
ACCTGAATCACGTAAATGTA

Downstream 100 bases:

>100_bases
GCGGGAGAGTGAGCGTATCATGAATTTACCTAGTAGTGGCCAGATATAACGTAAAGTACGTTTGACAATTTCTGGTGTCA
ACCACGAAAGAATTAGCAAC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKVLILNAGSSSQKSCLYEIPDDALLSEAPQPLWEGKVNWTQDRSVAEIEVKTARGETLHESIYGDSRQAHVTYMLYTLS
RGATKVIGQLSEIDVVGHRVVHGGQNYRHSVIITEEVKQAIAKLSNLAPAHNPAALEGIEAIEKSLGDVPQVAVFDTGFH
ATLPDAAAIYPGPFEWVEQGIRRYGFHGISHQYCSARAAQILGRDLASLRIITCHLGNGCSLAAIKNGRSIDTTMGFTPL
DGLMMGSRSGAIDPGIIVHLMRQSDYSAERLDYVLNKASGLRGISGVSSDLPQVIEAITQGNYRAQLAWDMYVHRLRSGI
GSMLASLGGLDVLVFTAGVGEKSAGIRQAACEAFGFLGLKLDPEKNQNKPVDIDIATADSTVRVLVIHTQEDWAIAQQCW
HLLKR

Sequences:

>Translated_405_residues
MKVLILNAGSSSQKSCLYEIPDDALLSEAPQPLWEGKVNWTQDRSVAEIEVKTARGETLHESIYGDSRQAHVTYMLYTLS
RGATKVIGQLSEIDVVGHRVVHGGQNYRHSVIITEEVKQAIAKLSNLAPAHNPAALEGIEAIEKSLGDVPQVAVFDTGFH
ATLPDAAAIYPGPFEWVEQGIRRYGFHGISHQYCSARAAQILGRDLASLRIITCHLGNGCSLAAIKNGRSIDTTMGFTPL
DGLMMGSRSGAIDPGIIVHLMRQSDYSAERLDYVLNKASGLRGISGVSSDLPQVIEAITQGNYRAQLAWDMYVHRLRSGI
GSMLASLGGLDVLVFTAGVGEKSAGIRQAACEAFGFLGLKLDPEKNQNKPVDIDIATADSTVRVLVIHTQEDWAIAQQCW
HLLKR
>Mature_405_residues
MKVLILNAGSSSQKSCLYEIPDDALLSEAPQPLWEGKVNWTQDRSVAEIEVKTARGETLHESIYGDSRQAHVTYMLYTLS
RGATKVIGQLSEIDVVGHRVVHGGQNYRHSVIITEEVKQAIAKLSNLAPAHNPAALEGIEAIEKSLGDVPQVAVFDTGFH
ATLPDAAAIYPGPFEWVEQGIRRYGFHGISHQYCSARAAQILGRDLASLRIITCHLGNGCSLAAIKNGRSIDTTMGFTPL
DGLMMGSRSGAIDPGIIVHLMRQSDYSAERLDYVLNKASGLRGISGVSSDLPQVIEAITQGNYRAQLAWDMYVHRLRSGI
GSMLASLGGLDVLVFTAGVGEKSAGIRQAACEAFGFLGLKLDPEKNQNKPVDIDIATADSTVRVLVIHTQEDWAIAQQCW
HLLKR

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=412, Percent_Identity=38.8349514563107, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI145693184, Length=315, Percent_Identity=44.4444444444444, Blast_Score=251, Evalue=4e-68,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43938; Mature: 43938

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLILNAGSSSQKSCLYEIPDDALLSEAPQPLWEGKVNWTQDRSVAEIEVKTARGETLH
CEEEEEECCCCCCCCEEEECCCHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCCHHH
ESIYGDSRQAHVTYMLYTLSRGATKVIGQLSEIDVVGHRVVHGGQNYRHSVIITEEVKQA
HHHCCCCCCEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHH
IAKLSNLAPAHNPAALEGIEAIEKSLGDVPQVAVFDTGFHATLPDAAAIYPGPFEWVEQG
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
IRRYGFHGISHQYCSARAAQILGRDLASLRIITCHLGNGCSLAAIKNGRSIDTTMGFTPL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCEECCCCCCCCC
DGLMMGSRSGAIDPGIIVHLMRQSDYSAERLDYVLNKASGLRGISGVSSDLPQVIEAITQ
CHHEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
GNYRAQLAWDMYVHRLRSGIGSMLASLGGLDVLVFTAGVGEKSAGIRQAACEAFGFLGLK
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
LDPEKNQNKPVDIDIATADSTVRVLVIHTQEDWAIAQQCWHLLKR
ECCCCCCCCCEEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLILNAGSSSQKSCLYEIPDDALLSEAPQPLWEGKVNWTQDRSVAEIEVKTARGETLH
CEEEEEECCCCCCCCEEEECCCHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCCHHH
ESIYGDSRQAHVTYMLYTLSRGATKVIGQLSEIDVVGHRVVHGGQNYRHSVIITEEVKQA
HHHCCCCCCEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHH
IAKLSNLAPAHNPAALEGIEAIEKSLGDVPQVAVFDTGFHATLPDAAAIYPGPFEWVEQG
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
IRRYGFHGISHQYCSARAAQILGRDLASLRIITCHLGNGCSLAAIKNGRSIDTTMGFTPL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCEECCCCCCCCC
DGLMMGSRSGAIDPGIIVHLMRQSDYSAERLDYVLNKASGLRGISGVSSDLPQVIEAITQ
CHHEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
GNYRAQLAWDMYVHRLRSGIGSMLASLGGLDVLVFTAGVGEKSAGIRQAACEAFGFLGLK
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
LDPEKNQNKPVDIDIATADSTVRVLVIHTQEDWAIAQQCWHLLKR
ECCCCCCCCCEEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA