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The map label for this gene is epsE [H]

Identifier: 17229936

GI number: 17229936

Start: 2937346

End: 2939358

Strand: Reverse

Name: epsE [H]

Synonym: all2444

Alternate gene names: 17229936

Gene position: 2939358-2937346 (Counterclockwise)

Preceding gene: 17229943

Following gene: 17229935

Centisome position: 45.83

GC content: 44.96

Gene sequence:

>2013_bases
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTTACTACAAGAACAGAGTTTTCGCCTTTCGGTAATAAGCTAGT
GCAGTCTGGCTACATTAATACCGAACAGATGAGACAGGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGAAG
TTTTGGAGTCAATTACTGGGCGACAATTGTCACCTGAGTATCTCAGGCAGTACAAGAAACAACAATTATTTGAACTGAAA
ATACTATACGGTGTTGAATACCTCGATCCGGAGGTCAACAATATCGGTAGCTCGATGGTGGGTAATTTAATTGAAACCCT
CATCCCAGTTGATATCTGCCGTCGTCATCGTTTAGTTCCACTATCTAAAAATGATGAACATACGCCGCCTTATGTTTTAG
TGGCGATGGTGGCTCCCGATAACTTGGAAGCATCGGATGACCTAAACCGCATCTTACGCCCCCAAGGTTTAACATTACAG
CGTATGGTAATTACCCAGGAGGATTACCAACAGCTAATTAACCAATATTTGGATGAATTAGCTGTGCGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTTGGCAATCTCGACCTAGAAGATGCTCCTGAAGAGA
TGGAGGCTGATTTAGGTTCAGCAATGAAGGGTGCAGAGGATGCACCAGTCATCAACCTCGTCAATAGAATTTTGGCGAAG
GCTTTGCATGAGAAGGTTTCTGATATCCACATCGAACCCCAAGAAGAAAACCTGCGGATTCGCTTCCGCAAGGATGGGGT
ACTGCGGGAGGCTTTTGACCCCCTACCGAAAAAAATTATTCCGGCTGTTACAGCTAGATTTAAAATTATCTCCAATTTAG
ATATTGCCGAGCGACGTTTACCCCAAGATGGACGTATCCGGCGGATGTTTGAGGGACGCAAGGTTGACTTCCGGGTGAAT
ACCTTACCTAGTCGCTATGGGGAAAAGGTAGTACTACGAATTTTGGATAATTCTTCTACCCAACTGGGGCTAAATAAGTT
AATCACTGACCCAGAAACATTGCAGATTGTCCAAGATATGGTGAGTAAGCCTTTCGGGTTGATTTTGGTGACTGGGCCGA
CTGGTTCAGGTAAAACCACTTCCTTGTATTCGGCACTGTCTGAGAAGAACTCACCAGGTATTAATATCAGTACGGTAGAA
GACCCGATTGAGTACAGTTTGCCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAAGGGCTAGATTTTGCCACAGCATT
ACGGGCTTTTTTGCGACAAGATCCTGATGTGTTGCTGGTGGGTGAAACAAGAGACAAAGAAACAGCGAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTGGTCTTAACTACATTACACACCAACGATGCCCCTGGTGCGATCGCTCGTTTGGGCGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTAATTGGGGTATTGGCACAACGTCTAGTGCGCCGTGTTTGTAGTGA
GTGCCGTATTGCTTATACTCCCACCACTGAGGAATTAGCTCGTTATGGAATGTCTGCTTCTCAAGAAGTGTCTGTCACCT
TCTATAAAGCTAACATTGTCCCAGCAGAAGCGATCGCCGAAGCCAAAGCTAAAAATGAGCTTTGTCCAGCTTGTAACGGT
GTCGGCTATAAAGGACGCTGTGGTGTTTATGAAGTGATGCGGGTTACAGAAAACCTACAAACTTTAATCAACGAAGAAGC
ACCAACAGAACGCATCAAAGAAGTAGCCGTAGAAGAAGGGATGAAAACCTTATTGGCTTATAGCCTAGACCTCGTTCGCC
AAGGTGCTACCACCTTAGAAGAAGTGGAACGGGTGACATTCACAGACACAGGTTTGGAAGCTGAGTTAAAAGCTAAACGC
AAGACTGGTCTTACCTGCCGGACGTGTAGTGCCATATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACACCCCG
TTTTGAAGATTAG

Upstream 100 bases:

>100_bases
TCGTAAAGTTTTGACTGGAATTTACTAAGTGTGGATGAAAATTTTTAGCCTCTGGCTGGGAATACTCTAAATAGAGGTTT
TCCCTACTCATTGCTCACCT

Downstream 100 bases:

>100_bases
TCATTGCATTTATTTGTTTGAATTTTGAATTGGAGCAAAGCGACCCTATGGAAATGATGATTGAAGACTTGATGGAGCAG
ATGATTGAAATGGGTGGTTC

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELK
ILYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIVPAEAIAEAKAKNELCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCSAILQPEWLDCPYCLTPRFED

Sequences:

>Translated_670_residues
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELK
ILYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIVPAEAIAEAKAKNELCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCSAILQPEWLDCPYCLTPRFED
>Mature_669_residues
TYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELKI
LYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQR
MVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAKA
LHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVED
PIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIVPAEAIAEAKAKNELCPACNGV
GYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKRK
TGLTCRTCSAILQPEWLDCPYCLTPRFED

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=431, Percent_Identity=46.8677494199536, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786296, Length=397, Percent_Identity=43.8287153652393, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082188, Length=198, Percent_Identity=38.8888888888889, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 75008; Mature: 74877

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITG
CCCCCCCCHHHHEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHC
RQLSPEYLRQYKKQQLFELKILYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVP
CCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQRMVITQEDYQQLINQYLDEL
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHH
AVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRL
HHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHC
PQDGRIRRMFEGRKVDFRVNTLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDM
CCCCCHHHHHCCCEEEEEEECCCHHHCCEEEEHEECCCCCHHHHHHHCCCHHHHHHHHHH
VSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVEDPIEYSLPGITQVQVIREKG
HCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHC
LDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
CCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHH
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIV
HCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCCCCEEEEEEEEECCC
PAEAIAEAKAKNELCPACNGVGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEG
CHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKRKTGLTCRTCSAILQPEWLDC
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCC
PYCLTPRFED
CCCCCCCCCC
>Mature Secondary Structure 
TYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITG
CCCCCCCHHHHEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHC
RQLSPEYLRQYKKQQLFELKILYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVP
CCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQRMVITQEDYQQLINQYLDEL
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHH
AVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRL
HHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHC
PQDGRIRRMFEGRKVDFRVNTLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDM
CCCCCHHHHHCCCEEEEEEECCCHHHCCEEEEHEECCCCCHHHHHHHCCCHHHHHHHHHH
VSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVEDPIEYSLPGITQVQVIREKG
HCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHC
LDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
CCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHH
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIV
HCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCCCCEEEEEEEEECCC
PAEAIAEAKAKNELCPACNGVGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEG
CHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKRKTGLTCRTCSAILQPEWLDC
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCC
PYCLTPRFED
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]