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The map label for this gene is pilT [H]

Identifier: 17229935

GI number: 17229935

Start: 2936180

End: 2937298

Strand: Reverse

Name: pilT [H]

Synonym: all2443

Alternate gene names: 17229935

Gene position: 2937298-2936180 (Counterclockwise)

Preceding gene: 17229936

Following gene: 17229934

Centisome position: 45.8

GC content: 43.61

Gene sequence:

>1119_bases
ATGGAAATGATGATTGAAGACTTGATGGAGCAGATGATTGAAATGGGTGGTTCGGATATGCACTTATCCGCAGGGTTGCC
CCCCTATTTCCGTATCAGTGGCAAACTCACACCCATAGGCGAAGAAGTACTGACCGCCGACCAGTGCCAAAGACTGATTT
TTAGTATGCTCAATAACACCCAACGTAAAACCTTAGAGCAGACTTGGGAGTTAGATTGTTCCTATGGTGTCAAAGGTTTG
GCTCGCTTCCGGGTCAATGTTTATAAAGAGCGTGGTGCTTATGCTGCTTGTTTAAGAGCGCTCAGTTCCAAAATTCCTAA
TTTTGAAAAGTTGGGTTTACCGGATGTTGTACGGGAAATGTGCGACAAACCTAGAGGGTTGATTCTCGTCACAGGGCCGA
CAGGTTCCGGTAAAACAACCACTCTGGCGGCAATGATTGACTTAATTAACCGCACGAAGGCAGAGCATATCCTGACAGTA
GAAGACCCCATTGAATTTGTATACGAACCAATTAAAAGCTTAGTTCACCAACGACAATTAGGTGAAGATACAAAAAGCTT
TGCTAACGCTTTAAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTTTGGTTGGGGAAATGCGGGATTTGGAGACGATTT
CTTTGGCGATTTCTGCCGCAGAAACCGGACACTTAGTATTTGGTACGCTGCACACTAGTTCTGCTTCTCAGACTGTTGAC
CGGATTATCGACGTTTTTCCCCACGAAAAACAAACTCAAGTACGGGTACAGTTATCTAACTCTTTAGTAGCTGTATTTAG
CCAAACATTAGTGCCGAAGAAAAATCCCAAGCCCGGTGAATACGGCCGTGTCATGGCACAAGAAATTATGATTATTACTC
CTGCTATTTCTAACTTAATTCGTGAAGGTAAAACCTCACAAATTTATTCCGCCATTCAGACTGGCGGTAAGTTAGGAATG
CAAACATTAGAAAAGGTTTTAGCTGATTATTACAAATCAGGCACAATTTCTTTTGAAGCAGCAATGTCTAAAACATCTAA
GCCAGATGAAATTCAACGCCTCATCGGTCCTTCTGCCCCACCACCAGGGGCTAAAGCCGGAGCCGTCAAAGCACATTAA

Upstream 100 bases:

>100_bases
AACCAGAATGGCTAGATTGTCCCTACTGTCTAACACCCCGTTTTGAAGATTAGTCATTGCATTTATTTGTTTGAATTTTG
AATTGGAGCAAAGCGACCCT

Downstream 100 bases:

>100_bases
AAAAAAGTCAATAGTTATTGTTTATTAGCCATGAATAATAACTATTAATTATTTTTGAATTTTGTAGCTTGCTTCTCGTA
GAGTATTTTGAATTTTGATA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKSGTISFEAAMSKTSKPDEIQRLIGPSAPPPGAKAGAVKAH

Sequences:

>Translated_372_residues
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKSGTISFEAAMSKTSKPDEIQRLIGPSAPPPGAKAGAVKAH
>Mature_372_residues
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKSGTISFEAAMSKTSKPDEIQRLIGPSAPPPGAKAGAVKAH

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=43.4650455927052, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1789723, Length=304, Percent_Identity=35.1973684210526, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786296, Length=393, Percent_Identity=29.2620865139949, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40823; Mature: 40823

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNT
CCHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCH
QRKTLEQTWELDCSYGVKGLARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREM
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHHHHH
CDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTVEDPIEFVYEPIKSLVHQRQL
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHC
GEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEECCCCCCEEEEEEECCCHHHHHH
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLI
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECHHHHHHH
REGKTSQIYSAIQTGGKLGMQTLEKVLADYYKSGTISFEAAMSKTSKPDEIQRLIGPSAP
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCHHHHHHHHCCCCC
PPGAKAGAVKAH
CCCCCCCCCCCC
>Mature Secondary Structure
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNT
CCHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCH
QRKTLEQTWELDCSYGVKGLARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREM
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHHHHH
CDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTVEDPIEFVYEPIKSLVHQRQL
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHC
GEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEECCCCCCEEEEEEECCCHHHHHH
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLI
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECHHHHHHH
REGKTSQIYSAIQTGGKLGMQTLEKVLADYYKSGTISFEAAMSKTSKPDEIQRLIGPSAP
HCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCHHHHHHHHCCCCC
PPGAKAGAVKAH
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]