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The map label for this gene is glnA

Identifier: 17229820

GI number: 17229820

Start: 2807483

End: 2808907

Strand: Direct

Name: glnA

Synonym: alr2328

Alternate gene names: 17229820

Gene position: 2807483-2808907 (Clockwise)

Preceding gene: 17229818

Following gene: 17229822

Centisome position: 43.77

GC content: 44.35

Gene sequence:

>1425_bases
ATGACAACCCCACAAGAAGTCTTGAAAAGAATTCAAGATGAAAAAATTGAGCTGATTGACCTCAAGTTCATCGATACAGT
AGGTACATGGCAGCACCTCACCTTGTACCAAAACCAAATCGATGAAAGCTCATTCTCTGATGGCGTACCTTTTGATGGTT
CCAGCATCCGGGGTTGGAAAGCAATCAACGAGTCAGACATGACAATGGTTCTCGATCCCAACACTGCTTGGATCGACCCA
TTCATGGAAGTGCCAACACTAAGTATTGTTTGTAGCATTAAAGAACCACGCACAGGAGAATGGTACAACCGTTGTCCACG
GGTTATTGCTCAAAAAGCAATAGACTACTTAGTATCTACGGGTATTGGTGATACAGCCTTCTTTGGCCCTGAAGCTGAAT
TTTTTATCTTTGATAGCGCCCGCTTTGCCCAAAACGCTAACGAAGGTTACTACTTCCTCGACTCTGTAGAGGGTGCTTGG
AATTCTGGTAAAGAAGGTACAGCAGATAAACCCAACTTGGCTTACAAACCACGTTTTAAAGAGGGTTATTTCCCTGTTTC
CCCCACAGATTCTTTCCAAGACATCCGGACAGAAATGTTGTTGACGATGGCGAAATTAGGCGTACCCATCGAAAAACATC
ACCACGAAGTTGCTACTGGTGGTCAGTGCGAGCTAGGCTTCCGCTTTGGTAAGTTAATCGAAGCTGCTGACTGGCTGATG
ATTTACAAATATGTCATCAAGAACGTTGCCAAAAAATATGGCAAAACCGTCACCTTCATGCCAAAACCAATTTTTGGTGA
TAACGGTTCTGGTATGCACTGTCACCAATCTATCTGGAAGGATGGCAAACCTCTCTTCGCAGGTGATCAGTATGCTGGCT
TAAGTGAAATGGGACTGTACTACATTGGTGGTCTTCTCAAGCACGCCCCAGCACTGTTGGCAATCACCAACCCCAGCACC
AACTCTTACAAGCGTCTAGTTCCTGGTTATGAAGCACCAGTTAACTTGGCTTACTCCCAAGGAAACCGTTCTGCTTCCAT
CCGTATTCCTCTGTCTGGCACTAACCCCAAAGCCAAGCGTTTAGAGTTCCGTTGTCCAGATGCTACCTCTAACCCTTATT
TGGCATTTGCTGCCATGCTGTGCGCTGGTATCGATGGTATCAAGAACAAAATTCATCCTGGTGAGCCATTAGATAAAAAC
ATCTATGAACTTTCTCCAGAAGAATTGGCAAAAGTTCCTTCCACACCTGGTTCTTTAGAACTAGCATTGGAAGCGCTAGA
AAACGACCACGCTTTCTTAACAGATACAGGCGTATTCACCGAAGACTTTATCCAAAACTGGATTGACTACAAACTAGCTA
ACGAAGTTAAACAGATGCAACTGCGTCCTCATCCTTATGAGTTCTCTATCTATTACGACGTTTAA

Upstream 100 bases:

>100_bases
ATATTTCAGGTGTGTCATCAACTTTGTTTCGCTCAGTAGGGCTTAGATGCTTTGGCAGATTCTGGTTTGAATTTAAGAAA
TTGGCAGAGAAGGAGTAACA

Downstream 100 bases:

>100_bases
TTAACACCTGTTAGCAATTTATAGCTGAAAGTTCAAATTTTGCCACCCTTATGGGTGGTTTTTTGTCTTCTGTAAAAATT
ATGTATAAGTATACTAGAGG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTTPQEVLKRIQDEKIELIDLKFIDTVGTWQHLTLYQNQIDESSFSDGVPFDGSSIRGWKAINESDMTMVLDPNTAWIDP
FMEVPTLSIVCSIKEPRTGEWYNRCPRVIAQKAIDYLVSTGIGDTAFFGPEAEFFIFDSARFAQNANEGYYFLDSVEGAW
NSGKEGTADKPNLAYKPRFKEGYFPVSPTDSFQDIRTEMLLTMAKLGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLM
IYKYVIKNVAKKYGKTVTFMPKPIFGDNGSGMHCHQSIWKDGKPLFAGDQYAGLSEMGLYYIGGLLKHAPALLAITNPST
NSYKRLVPGYEAPVNLAYSQGNRSASIRIPLSGTNPKAKRLEFRCPDATSNPYLAFAAMLCAGIDGIKNKIHPGEPLDKN
IYELSPEELAKVPSTPGSLELALEALENDHAFLTDTGVFTEDFIQNWIDYKLANEVKQMQLRPHPYEFSIYYDV

Sequences:

>Translated_474_residues
MTTPQEVLKRIQDEKIELIDLKFIDTVGTWQHLTLYQNQIDESSFSDGVPFDGSSIRGWKAINESDMTMVLDPNTAWIDP
FMEVPTLSIVCSIKEPRTGEWYNRCPRVIAQKAIDYLVSTGIGDTAFFGPEAEFFIFDSARFAQNANEGYYFLDSVEGAW
NSGKEGTADKPNLAYKPRFKEGYFPVSPTDSFQDIRTEMLLTMAKLGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLM
IYKYVIKNVAKKYGKTVTFMPKPIFGDNGSGMHCHQSIWKDGKPLFAGDQYAGLSEMGLYYIGGLLKHAPALLAITNPST
NSYKRLVPGYEAPVNLAYSQGNRSASIRIPLSGTNPKAKRLEFRCPDATSNPYLAFAAMLCAGIDGIKNKIHPGEPLDKN
IYELSPEELAKVPSTPGSLELALEALENDHAFLTDTGVFTEDFIQNWIDYKLANEVKQMQLRPHPYEFSIYYDV
>Mature_473_residues
TTPQEVLKRIQDEKIELIDLKFIDTVGTWQHLTLYQNQIDESSFSDGVPFDGSSIRGWKAINESDMTMVLDPNTAWIDPF
MEVPTLSIVCSIKEPRTGEWYNRCPRVIAQKAIDYLVSTGIGDTAFFGPEAEFFIFDSARFAQNANEGYYFLDSVEGAWN
SGKEGTADKPNLAYKPRFKEGYFPVSPTDSFQDIRTEMLLTMAKLGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLMI
YKYVIKNVAKKYGKTVTFMPKPIFGDNGSGMHCHQSIWKDGKPLFAGDQYAGLSEMGLYYIGGLLKHAPALLAITNPSTN
SYKRLVPGYEAPVNLAYSQGNRSASIRIPLSGTNPKAKRLEFRCPDATSNPYLAFAAMLCAGIDGIKNKIHPGEPLDKNI
YELSPEELAKVPSTPGSLELALEALENDHAFLTDTGVFTEDFIQNWIDYKLANEVKQMQLRPHPYEFSIYYDV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=245, Percent_Identity=29.7959183673469, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1790301, Length=472, Percent_Identity=54.8728813559322, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI87081870, Length=187, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_NOSS1 (P00964)

Other databases:

- EMBL:   X00147
- EMBL:   BA000019
- PIR:   A01192
- PIR:   AI2096
- RefSeq:   NP_486368.1
- ProteinModelPortal:   P00964
- SMR:   P00964
- STRING:   P00964
- GeneID:   1105925
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr2328
- NMPDR:   fig|103690.1.peg.2635
- eggNOG:   COG0174
- HOGENOM:   HBG553755
- OMA:   FAEGTMF
- PhylomeDB:   P00964
- ProtClustDB:   CLSK892650
- BioCyc:   NSP103690:ALR2328-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 53137; Mature: 53006

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00180 GLNA_1; PS00182 GLNA_ADENYLATION; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPQEVLKRIQDEKIELIDLKFIDTVGTWQHLTLYQNQIDESSFSDGVPFDGSSIRGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AINESDMTMVLDPNTAWIDPFMEVPTLSIVCSIKEPRTGEWYNRCPRVIAQKAIDYLVST
CCCCCCCEEEECCCCCCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIGDTAFFGPEAEFFIFDSARFAQNANEGYYFLDSVEGAWNSGKEGTADKPNLAYKPRFK
CCCCCEEECCCCEEEEEECHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
EGYFPVSPTDSFQDIRTEMLLTMAKLGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCCEEECHHHHHHHHHHHHHH
IYKYVIKNVAKKYGKTVTFMPKPIFGDNGSGMHCHQSIWKDGKPLFAGDQYAGLSEMGLY
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCCCHHHHHHH
YIGGLLKHAPALLAITNPSTNSYKRLVPGYEAPVNLAYSQGNRSASIRIPLSGTNPKAKR
HHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRCPDATSNPYLAFAAMLCAGIDGIKNKIHPGEPLDKNIYELSPEELAKVPSTPGSLE
EEEECCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCCCHHH
LALEALENDHAFLTDTGVFTEDFIQNWIDYKLANEVKQMQLRPHPYEFSIYYDV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
TTPQEVLKRIQDEKIELIDLKFIDTVGTWQHLTLYQNQIDESSFSDGVPFDGSSIRGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AINESDMTMVLDPNTAWIDPFMEVPTLSIVCSIKEPRTGEWYNRCPRVIAQKAIDYLVST
CCCCCCCEEEECCCCCCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GIGDTAFFGPEAEFFIFDSARFAQNANEGYYFLDSVEGAWNSGKEGTADKPNLAYKPRFK
CCCCCEEECCCCEEEEEECHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
EGYFPVSPTDSFQDIRTEMLLTMAKLGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHCCCCCEEECHHHHHHHHHHHHHH
IYKYVIKNVAKKYGKTVTFMPKPIFGDNGSGMHCHQSIWKDGKPLFAGDQYAGLSEMGLY
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCCCHHHHHHH
YIGGLLKHAPALLAITNPSTNSYKRLVPGYEAPVNLAYSQGNRSASIRIPLSGTNPKAKR
HHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRCPDATSNPYLAFAAMLCAGIDGIKNKIHPGEPLDKNIYELSPEELAKVPSTPGSLE
EEEECCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCCHHHCCHHHHHHCCCCCCHHH
LALEALENDHAFLTDTGVFTEDFIQNWIDYKLANEVKQMQLRPHPYEFSIYYDV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840