Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 17229502

GI number: 17229502

Start: 2404812

End: 2405975

Strand: Direct

Name: dnaN [H]

Synonym: alr2010

Alternate gene names: 17229502

Gene position: 2404812-2405975 (Clockwise)

Preceding gene: 17229501

Following gene: 17229506

Centisome position: 37.49

GC content: 44.16

Gene sequence:

>1164_bases
ATGAAATTAGTTTGCGCCCAAAGCGACCTCAGTACAAACCTTTCCCTCGTCAATCGTGCTGTACCATCACGACCGACTCA
TCCAGTACTTGCTAACGTGCTGTTGCAAGCGGATGCTGAAACTAATCAAGTAAGCTTAACAGCCTTTGATCTTAGCTTGG
GTATCCGTACTAGTTTTAATGCTGACGTATGGCAAAGTGGAGCGATCGCACTTCCTGCTAAATTATTGGTAGATATCACC
TCACGTCTGCCAGAGGGAGAAATTACCCTAGATGATGAATCAGCCACCGACGGTACAGCAACCGGAGAAGGTTTAATAGT
TAGCCTGACCCCAAAAACCGGAAAGTACCAATTACGAGCAATGGGAGCGGGAGAGTTTCCCGAATTACCCCTAATTGAGA
ACACCACAGCTATTTATCTCACAGCTACTTCCTTAATTGAAGGATTGCGGGGTTCATTATTTGCTACTAGTGGCGACGAA
ACTAAGCAAGTCCTCACAGGAGTACATCTGACAGTTAAACAAGATACCTTGGAATTTGCCGCTACTGATGGGCATCGTCT
AGCCGTAGTCGAAACCACTAATGAGCGTCCCTTAGAAGATAACGACCAGCAATTAGAGGTAACAGTACCAGCTAGAGCCT
TACGCGAACTAGAAAGGATGTTGGCTCATAATGCCGCCTCTGAGGAACCCATAGCGTTATATTTAGACCAAGGTCAAGTC
GTCTTTGCATGGCAAAACCAACGCCTAACTAGTCGCACATTAGAAGGTCAATACCCAGCTTATCGCCAACTTATTCCCCG
CCAATTTGAGCGACAAGTGACCATTGAACGCCGACAATTCTTAAGCACATTAGAAAGAATTGCTGTACTAGCAGACCAGA
AAAATAATATCGTCAAGCTAACTATTGATAGTACTGCCCAAGAGCTGACCTTATCTTGTGAAGCTCAAGAAATGGGCAGT
GGGAGAGAGTCGATGAATGCGGAAATTGCTGGGGAAGATATAGAAATTGCTTTTAATGTCAAATACTTAATGGAAGGACT
CAAAGCCTTACCATCCCCAGAAATTCAAATGCACATCAACCAAAATCTAACTCCAGTAATTTTCACTCCCCTGGGTGGTT
TGAAGATGACTTATTTGGCTATGCCGGTGCAATTAAGAAATTAA

Upstream 100 bases:

>100_bases
GCAAATTCAGATAAGATTTTATACCACTCACTGTTTTAAAATTAAGACTACAAACTACTTTTCTTAATTTTCAATTTCCA
CTCCCCATTCCCCACTCCCC

Downstream 100 bases:

>100_bases
CTAATTGTGGGGAATGGGTAATGGGTAATGGGTAATGGGTAATGAAAGAGAAGTTAATTTGTCCCCAATCACCAATTACC
AATCACCAATCACCTAGCTA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFNADVWQSGAIALPAKLLVDIT
SRLPEGEITLDDESATDGTATGEGLIVSLTPKTGKYQLRAMGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDE
TKQVLTGVHLTVKQDTLEFAATDGHRLAVVETTNERPLEDNDQQLEVTVPARALRELERMLAHNAASEEPIALYLDQGQV
VFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVLADQKNNIVKLTIDSTAQELTLSCEAQEMGS
GRESMNAEIAGEDIEIAFNVKYLMEGLKALPSPEIQMHINQNLTPVIFTPLGGLKMTYLAMPVQLRN

Sequences:

>Translated_387_residues
MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFNADVWQSGAIALPAKLLVDIT
SRLPEGEITLDDESATDGTATGEGLIVSLTPKTGKYQLRAMGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDE
TKQVLTGVHLTVKQDTLEFAATDGHRLAVVETTNERPLEDNDQQLEVTVPARALRELERMLAHNAASEEPIALYLDQGQV
VFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVLADQKNNIVKLTIDSTAQELTLSCEAQEMGS
GRESMNAEIAGEDIEIAFNVKYLMEGLKALPSPEIQMHINQNLTPVIFTPLGGLKMTYLAMPVQLRN
>Mature_387_residues
MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFNADVWQSGAIALPAKLLVDIT
SRLPEGEITLDDESATDGTATGEGLIVSLTPKTGKYQLRAMGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDE
TKQVLTGVHLTVKQDTLEFAATDGHRLAVVETTNERPLEDNDQQLEVTVPARALRELERMLAHNAASEEPIALYLDQGQV
VFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVLADQKNNIVKLTIDSTAQELTLSCEAQEMGS
GRESMNAEIAGEDIEIAFNVKYLMEGLKALPSPEIQMHINQNLTPVIFTPLGGLKMTYLAMPVQLRN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=388, Percent_Identity=24.7422680412371, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42485; Mature: 42485

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFN
CEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECEEECCC
ADVWQSGAIALPAKLLVDITSRLPEGEITLDDESATDGTATGEGLIVSLTPKTGKYQLRA
CCHHCCCCEECHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEE
MGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDETKQVLTGVHLTVKQDTLEFA
ECCCCCCCCCEECCCCEEEEEHHHHHHHHCCCEEECCCCHHHHHHHCEEEEEECCCEEEE
ATDGHRLAVVETTNERPLEDNDQQLEVTVPARALRELERMLAHNAASEEPIALYLDQGQV
ECCCCEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
VFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVLADQKNNIVKL
EEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TIDSTAQELTLSCEAQEMGSGRESMNAEIAGEDIEIAFNVKYLMEGLKALPSPEIQMHIN
EECCCCEEEEEEECHHHHCCCHHHCCCEECCCCEEEEEEHHHHHHHHHHCCCCCEEEEEC
QNLTPVIFTPLGGLKMTYLAMPVQLRN
CCCCEEEEECCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFN
CEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECEEECCC
ADVWQSGAIALPAKLLVDITSRLPEGEITLDDESATDGTATGEGLIVSLTPKTGKYQLRA
CCHHCCCCEECHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEE
MGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDETKQVLTGVHLTVKQDTLEFA
ECCCCCCCCCEECCCCEEEEEHHHHHHHHCCCEEECCCCHHHHHHHCEEEEEECCCEEEE
ATDGHRLAVVETTNERPLEDNDQQLEVTVPARALRELERMLAHNAASEEPIALYLDQGQV
ECCCCEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
VFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVLADQKNNIVKL
EEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TIDSTAQELTLSCEAQEMGSGRESMNAEIAGEDIEIAFNVKYLMEGLKALPSPEIQMHIN
EECCCCEEEEEEECHHHHCCCHHHCCCEECCCCEEEEEEHHHHHHHHHHCCCCCEEEEEC
QNLTPVIFTPLGGLKMTYLAMPVQLRN
CCCCEEEEECCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]