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The map label for this gene is pfkA

Identifier: 17229405

GI number: 17229405

Start: 2293110

End: 2294183

Strand: Direct

Name: pfkA

Synonym: alr1913

Alternate gene names: 17229405

Gene position: 2293110-2294183 (Clockwise)

Preceding gene: 17229404

Following gene: 17229407

Centisome position: 35.75

GC content: 46.28

Gene sequence:

>1074_bases
ATGCGTAAACGAATTGGTATCCTCACCAGTGGCGGCGACTGTCCAGGGCTTAACTGCGTAATTCGCGCAGTTGTTAGCCA
TGCAACGCTTACTTATGATTGGGAGGTATTGGGTATACCTTATGCCACTCAGGGTTTACGGGAGCGTCAAGCGATCGCTC
TCAATATGCACGGTTGGGATTTGCGCGGTATCGATCCTCTGCTGAATATGGGTGGTACGATTTTAGGCACAATTAATAAG
GGTGATACCTTAGCCCATGTGGATGAAATGCTTGCCAGTTATCAGGCTTTGGCTTTGGATGCTTTGATTGTCATTGGCGG
TGATGGTAGTCTGGGTATCCTCCATGAACTGGCTAGCCGAGGTAATTGGAATTTAGTGGCGATTCCCAAAACTATAGATA
ATGATGTGGCTTTGACAGAACGCGCCGTGGGTTTTGATACGGCGGTGAATACAATTGTTGATGCCCTCAATCGCTTGACT
TTTACGGCTGCAAGTCACGATCGCGTGATGATTGTCGAAGTTATGGGGCGCAGCGCCGGACATCTAGCCCTACACGCGGG
TATTGCTGGCGGTGCAGATGTAATTTTAATCCCCGAAATTTCATACACAATTAGCGGTTTATGTCAACATATAGCTGAAT
TGCGCGATCGCTGGCAACGCAAATTTGCGATCGTTGTTGTTGCCGAAGGTGCGAAACTATGTCTGGAAGATGTGCAAGAA
AATATTGCCTCTTCTTGTGCGCCTTCTAAATGCGGTCGCGGTCAATATATTGCGGATCAAATTGCTCAATGTAGTAAAAA
TCTCATCGATACCAGAGTTTCTGTATTGGGACACATTCAGCGTGGCGGTATCCCATCAGCTTTAGACCGTTTAACAGCAA
CAGTTTTTGGGAAAACAGCCGTTGATTTAATAGCTCAAGGTAAATTTGGGCAGATGGTAGCTTGGCAAAATGGCGAAGCT
ATCCCAGTACCCATTCAGGACGTTGTCGCTCAAAGTCCTTTACACGTAAATCCCCAAGGTTCTTTGGTACAGAGCGCTCG
TTGTTTGGGTATTTATGTAGGAGAAAAAACATAA

Upstream 100 bases:

>100_bases
CTAACTGTCTTTAATTTTGAATTTTGAATTTTGAATTTTGAATTTTGAATTTTGAATTTTGAATTTTGAATTGTTAAGAG
AGGCTAACGCCAAAACTAAT

Downstream 100 bases:

>100_bases
TTCTTCCCCCCATTTACTCTAACCGCAACTTCTAAGTACGTGATGTAGGCTATTTAATAATTCCTTGGCTGTAAAAGGTT
TAGGTAAAACTTGCTGCACA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRKRIGILTSGGDCPGLNCVIRAVVSHATLTYDWEVLGIPYATQGLRERQAIALNMHGWDLRGIDPLLNMGGTILGTINK
GDTLAHVDEMLASYQALALDALIVIGGDGSLGILHELASRGNWNLVAIPKTIDNDVALTERAVGFDTAVNTIVDALNRLT
FTAASHDRVMIVEVMGRSAGHLALHAGIAGGADVILIPEISYTISGLCQHIAELRDRWQRKFAIVVVAEGAKLCLEDVQE
NIASSCAPSKCGRGQYIADQIAQCSKNLIDTRVSVLGHIQRGGIPSALDRLTATVFGKTAVDLIAQGKFGQMVAWQNGEA
IPVPIQDVVAQSPLHVNPQGSLVQSARCLGIYVGEKT

Sequences:

>Translated_357_residues
MRKRIGILTSGGDCPGLNCVIRAVVSHATLTYDWEVLGIPYATQGLRERQAIALNMHGWDLRGIDPLLNMGGTILGTINK
GDTLAHVDEMLASYQALALDALIVIGGDGSLGILHELASRGNWNLVAIPKTIDNDVALTERAVGFDTAVNTIVDALNRLT
FTAASHDRVMIVEVMGRSAGHLALHAGIAGGADVILIPEISYTISGLCQHIAELRDRWQRKFAIVVVAEGAKLCLEDVQE
NIASSCAPSKCGRGQYIADQIAQCSKNLIDTRVSVLGHIQRGGIPSALDRLTATVFGKTAVDLIAQGKFGQMVAWQNGEA
IPVPIQDVVAQSPLHVNPQGSLVQSARCLGIYVGEKT
>Mature_357_residues
MRKRIGILTSGGDCPGLNCVIRAVVSHATLTYDWEVLGIPYATQGLRERQAIALNMHGWDLRGIDPLLNMGGTILGTINK
GDTLAHVDEMLASYQALALDALIVIGGDGSLGILHELASRGNWNLVAIPKTIDNDVALTERAVGFDTAVNTIVDALNRLT
FTAASHDRVMIVEVMGRSAGHLALHAGIAGGADVILIPEISYTISGLCQHIAELRDRWQRKFAIVVVAEGAKLCLEDVQE
NIASSCAPSKCGRGQYIADQIAQCSKNLIDTRVSVLGHIQRGGIPSALDRLTATVFGKTAVDLIAQGKFGQMVAWQNGEA
IPVPIQDVVAQSPLHVNPQGSLVQSARCLGIYVGEKT

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI48762920, Length=326, Percent_Identity=33.1288343558282, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI11321601, Length=327, Percent_Identity=33.9449541284404, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=31.6804407713499, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=31.6804407713499, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=31.6804407713499, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=31.6804407713499, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI1790350, Length=333, Percent_Identity=36.6366366366366, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17558788, Length=338, Percent_Identity=31.3609467455621, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25147584, Length=366, Percent_Identity=28.6885245901639, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI72003601, Length=202, Percent_Identity=33.1683168316832, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321679, Length=333, Percent_Identity=32.4324324324324, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323861, Length=344, Percent_Identity=33.1395348837209, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17647809, Length=366, Percent_Identity=30.0546448087432, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24652337, Length=366, Percent_Identity=30.0546448087432, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28573326, Length=366, Percent_Identity=29.2349726775956, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF2_NOSS1 (Q8YVR1)

Other databases:

- EMBL:   BA000019
- PIR:   AC2045
- RefSeq:   NP_485953.1
- ProteinModelPortal:   Q8YVR1
- SMR:   Q8YVR1
- STRING:   Q8YVR1
- GeneID:   1105505
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1913
- NMPDR:   fig|103690.1.peg.2220
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   NMGGTIL
- ProtClustDB:   PRK03202
- BioCyc:   NSP103690:ALR1913-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 38007; Mature: 38007

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 133-133 BINDING 168-168 BINDING 272-272 BINDING 278-278 BINDING 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRIGILTSGGDCPGLNCVIRAVVSHATLTYDWEVLGIPYATQGLRERQAIALNMHGWD
CCCEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEEEEECCHHHHCHHCCCEEEEEECCCC
LRGIDPLLNMGGTILGTINKGDTLAHVDEMLASYQALALDALIVIGGDGSLGILHELASR
CCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHC
GNWNLVAIPKTIDNDVALTERAVGFDTAVNTIVDALNRLTFTAASHDRVMIVEVMGRSAG
CCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCC
HLALHAGIAGGADVILIPEISYTISGLCQHIAELRDRWQRKFAIVVVAEGAKLCLEDVQE
CEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHH
NIASSCAPSKCGRGQYIADQIAQCSKNLIDTRVSVLGHIQRGGIPSALDRLTATVFGKTA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VDLIAQGKFGQMVAWQNGEAIPVPIQDVVAQSPLHVNPQGSLVQSARCLGIYVGEKT
HHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCEECCCCCHHHCCEEEEEEECCCC
>Mature Secondary Structure
MRKRIGILTSGGDCPGLNCVIRAVVSHATLTYDWEVLGIPYATQGLRERQAIALNMHGWD
CCCEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEEEEECCHHHHCHHCCCEEEEEECCCC
LRGIDPLLNMGGTILGTINKGDTLAHVDEMLASYQALALDALIVIGGDGSLGILHELASR
CCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHC
GNWNLVAIPKTIDNDVALTERAVGFDTAVNTIVDALNRLTFTAASHDRVMIVEVMGRSAG
CCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCC
HLALHAGIAGGADVILIPEISYTISGLCQHIAELRDRWQRKFAIVVVAEGAKLCLEDVQE
CEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHH
NIASSCAPSKCGRGQYIADQIAQCSKNLIDTRVSVLGHIQRGGIPSALDRLTATVFGKTA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VDLIAQGKFGQMVAWQNGEAIPVPIQDVVAQSPLHVNPQGSLVQSARCLGIYVGEKT
HHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCEECCCCCHHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840