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The map label for this gene is tgt

Identifier: 17229377

GI number: 17229377

Start: 2251283

End: 2252302

Strand: Direct

Name: tgt

Synonym: alr1885

Alternate gene names: 17229377

Gene position: 2251283-2252302 (Clockwise)

Preceding gene: 17229375

Following gene: 17229382

Centisome position: 35.1

GC content: 46.47

Gene sequence:

>1020_bases
ATGCCAGTGGGAACCCTGGCTAATGTAAAGACTGTCACACCAGCACAGCTGAAGGAGACTGGGGCGCAGATGGTTCTCTC
TAATACTTATCATCTCCATCTCCAACCAGGGGAAGCCATTGTGGCTGGTGGTGGTGGATTGCATAAATTTATGGGCTGGA
ATGGGCCGATGCTCACCGATTCTGGTGGGTTTCAGGTTTTCAGCTTGAGCGAAATGAGAAAAATTACGGAAGAAGGTGTG
ACTTTTCGCTCACCTCGTGACGGACAAATTATTAAATTAACGCCGGAACGCTCTATAGAAATTCAAAATATTTTAGGGGC
TGACGTTATCATGGCGTTCGATGAATGTCCCCCCTACCCAGCTAATCGCCAGGAAGTAGAAGCGGCGACTGAGCGGACTT
ACCGTTGGTTGGAACGCTGCATTACGGCTCATCAACGCCAGGATCAAGCGTTGTTCGGTATCGTTCAAGGTGGGGTATAT
CTGGATTTACGGGCTAAAGCTGCCAATACTTTAACTGAGTTAGATTTACCTGGTTATGCCATTGGTGGGGTGAGTGTGGG
CGAACCACCAGAAATGATGGCGCAAATTGTGCAAGCTACAGCACCACTTTTACCAGCACACAAGCCGCGTTACTTGATGG
GTGTGGGAACTTATCGAGAGATGGTAATTGCGATCGCCTCTGGTATAGACTTATTTGATTGCGTCATTCCCACCCGTTGG
GCGAGACACGGTACAGCAATGGTCAAAGGCGAACGTTGGAACTTAAAAAATGCTAAGTTCCGTGAAGATTTTGCCCCAAT
AGATGAAACTTGCCCTTGTTATGCTTGTCAAAATTTCAGCCGTGCCTATATCTCTCATCTAGTGCGATCGCAAGAAATTT
TAGCCTACACCTTATTGAGCATTCACAACATTACCGAACTCATTCGCTTTACCCAAAAAATTCGTGAAGCAATATTAAGC
GACCGCTTTTTGGAAGAATTTGGTCACTGGTTGAACTCGGCGGAAACTGATAATAGGTAA

Upstream 100 bases:

>100_bases
TGCCATATTTTCCTTTCAATCCCTAGCTCGTTGTAGCCAAACAAAAGCCAGATCTGGGATTTTTTTAACTCCCCACGGAA
TTGTCGAAACTCCGAGATTT

Downstream 100 bases:

>100_bases
TGAGTAATTGGTAATTGGTAATTGTTTTGTATCATTAGCAATTACCAATTTTCACCGATATCATTTAGTCTCAAAACAGA
TTGATATACTAAAGGACTTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGV
TFRSPRDGQIIKLTPERSIEIQNILGADVIMAFDECPPYPANRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVY
LDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMAQIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRW
ARHGTAMVKGERWNLKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILS
DRFLEEFGHWLNSAETDNR

Sequences:

>Translated_339_residues
MPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGV
TFRSPRDGQIIKLTPERSIEIQNILGADVIMAFDECPPYPANRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVY
LDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMAQIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRW
ARHGTAMVKGERWNLKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILS
DRFLEEFGHWLNSAETDNR
>Mature_338_residues
PVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGVT
FRSPRDGQIIKLTPERSIEIQNILGADVIMAFDECPPYPANRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYL
DLRAKAANTLTELDLPGYAIGGVSVGEPPEMMAQIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWA
RHGTAMVKGERWNLKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILSD
RFLEEFGHWLNSAETDNR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=324, Percent_Identity=46.2962962962963, Blast_Score=305, Evalue=3e-83,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=25, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786607, Length=332, Percent_Identity=48.7951807228916, Blast_Score=327, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17544680, Length=331, Percent_Identity=45.0151057401813, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=234, Percent_Identity=26.9230769230769, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=324, Percent_Identity=47.2222222222222, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21355311, Length=252, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_NOSS1 (Q8YVT9)

Other databases:

- EMBL:   BA000019
- PIR:   AG2041
- RefSeq:   NP_485925.1
- ProteinModelPortal:   Q8YVT9
- SMR:   Q8YVT9
- STRING:   Q8YVT9
- GeneID:   1105477
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1885
- NMPDR:   fig|103690.1.peg.2192
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   RCIAAHK
- PhylomeDB:   Q8YVT9
- ProtClustDB:   PRK00112
- BioCyc:   NSP103690:ALR1885-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 37811; Mature: 37680

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTD
CCCCCCCCCCCCCHHHHHHHHHHEEEECEEEEEECCCCEEEECCCCHHHHCCCCCCEEEC
SGGFQVFSLSEMRKITEEGVTFRSPRDGQIIKLTPERSIEIQNILGADVIMAFDECPPYP
CCCEEEEEHHHHHHHHHCCCEECCCCCCCEEEECCCCCEEHHHHHCHHHHHEECCCCCCC
ANRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCEEEEEEHHHHHHHHHCCCCCEE
IGGVSVGEPPEMMAQIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRW
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCHHH
ARHGTAMVKGERWNLKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLS
HHCCCEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHNITELIRFTQKIREAILSDRFLEEFGHWLNSAETDNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTD
CCCCCCCCCCCCHHHHHHHHHHEEEECEEEEEECCCCEEEECCCCHHHHCCCCCCEEEC
SGGFQVFSLSEMRKITEEGVTFRSPRDGQIIKLTPERSIEIQNILGADVIMAFDECPPYP
CCCEEEEEHHHHHHHHHCCCEECCCCCCCEEEECCCCCEEHHHHHCHHHHHEECCCCCCC
ANRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCEEEEEEHHHHHHHHHCCCCCEE
IGGVSVGEPPEMMAQIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRW
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCHHH
ARHGTAMVKGERWNLKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLS
HHCCCEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHNITELIRFTQKIREAILSDRFLEEFGHWLNSAETDNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840