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The map label for this gene is trmB

Identifier: 17229337

GI number: 17229337

Start: 2207650

End: 2208309

Strand: Direct

Name: trmB

Synonym: alr1845

Alternate gene names: 17229337

Gene position: 2207650-2208309 (Clockwise)

Preceding gene: 17229328

Following gene: 17229342

Centisome position: 34.42

GC content: 45.61

Gene sequence:

>660_bases
GTGATTTCAGGAGTGTCAGCTTTGCCATTTGTTCGCGTCCGTCAGCACGTCAACCCACTAGCGCAAAAATATCTCACCCC
AGCCAATCCTCTGGAATGGGAAAAAGCTTATAGCACTCCGCACCAACCGCTACACCTGGATATTGGTTGTGCTAGGGGTA
GATTTGTGTTGCAAATGGCGCAGGTAGAACCCAATTGGAATTTCCTGGGGTTAGAAATTAGAGAATCTTTGGTAATAGAG
GCAAATCAGTTTCGTTCTCAGTTGGGTTTAACCAACCTCCACTATTTATATTGCAACGCCAATAATTCACTACAGCCCCT
ATTATCCTCTTTGCCTACAGGGATTTTACAGCGTGTGACCATTCAATTCCCTGACCCTTGGTTTAAAACTCGCCACGCTA
AACGCCGTGTAGTCCAACCAGAGTTAGTTCAAGATATAGCTAATTATTTGGCCGTGGGCGGCGTGGTATTTTTGCAATCA
GATATGGAATTTGTGGCTGTGGAAATGTGCGATCGCTTTGCTGCTAATCCAGCTTTTAAGAAAGTTGGCACAGGAGAATG
GTTAACCGAAAATCCCCTCCCAGTGGCTACAGAGAGAGAAACCACAACCCAGAATCGGGGAGAACCAGTTTATCGGGCTT
TGTTTGAGAGAAGCAGTTAA

Upstream 100 bases:

>100_bases
GATAAGGGACTGGAGATTGGTAGAAAAGAACAACAGTCAACGGTCAACAGTCAACAGTCAACCAACAACTGACAACTGAC
TAATTGCTATATTTAAAGTT

Downstream 100 bases:

>100_bases
GTATTTACACTGACCCGCTTAGTGCTGGGGATAGGTGGAAAATACTGGCCACTACGGAAATTAATTTGTTGGGATCAATC
GGTTTGGAGAGGTGATGCTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MISGVSALPFVRVRQHVNPLAQKYLTPANPLEWEKAYSTPHQPLHLDIGCARGRFVLQMAQVEPNWNFLGLEIRESLVIE
ANQFRSQLGLTNLHYLYCNANNSLQPLLSSLPTGILQRVTIQFPDPWFKTRHAKRRVVQPELVQDIANYLAVGGVVFLQS
DMEFVAVEMCDRFAANPAFKKVGTGEWLTENPLPVATERETTTQNRGEPVYRALFERSS

Sequences:

>Translated_219_residues
MISGVSALPFVRVRQHVNPLAQKYLTPANPLEWEKAYSTPHQPLHLDIGCARGRFVLQMAQVEPNWNFLGLEIRESLVIE
ANQFRSQLGLTNLHYLYCNANNSLQPLLSSLPTGILQRVTIQFPDPWFKTRHAKRRVVQPELVQDIANYLAVGGVVFLQS
DMEFVAVEMCDRFAANPAFKKVGTGEWLTENPLPVATERETTTQNRGEPVYRALFERSS
>Mature_219_residues
MISGVSALPFVRVRQHVNPLAQKYLTPANPLEWEKAYSTPHQPLHLDIGCARGRFVLQMAQVEPNWNFLGLEIRESLVIE
ANQFRSQLGLTNLHYLYCNANNSLQPLLSSLPTGILQRVTIQFPDPWFKTRHAKRRVVQPELVQDIANYLAVGGVVFLQS
DMEFVAVEMCDRFAANPAFKKVGTGEWLTENPLPVATERETTTQNRGEPVYRALFERSS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Homo sapiens, GI167004078, Length=255, Percent_Identity=27.843137254902, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789330, Length=177, Percent_Identity=29.9435028248588, Blast_Score=80, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17536689, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320000, Length=217, Percent_Identity=27.1889400921659, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20128915, Length=234, Percent_Identity=25.2136752136752, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NOSS1 (Q8YVX4)

Other databases:

- EMBL:   BA000019
- PIR:   AG2036
- RefSeq:   NP_485885.1
- ProteinModelPortal:   Q8YVX4
- SMR:   Q8YVX4
- STRING:   Q8YVX4
- GeneID:   1105437
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1845
- NMPDR:   fig|103690.1.peg.2152
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   KRRVVQP
- PhylomeDB:   Q8YVX4
- ProtClustDB:   PRK00121
- BioCyc:   NSP103690:ALR1845-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24848; Mature: 24848

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: BINDING 47-47 BINDING 72-72 BINDING 99-99 BINDING 125-125 BINDING 129-129 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGVSALPFVRVRQHVNPLAQKYLTPANPLEWEKAYSTPHQPLHLDIGCARGRFVLQMA
CCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCCEEEEEE
QVEPNWNFLGLEIRESLVIEANQFRSQLGLTNLHYLYCNANNSLQPLLSSLPTGILQRVT
EECCCCCEEEHEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHEE
IQFPDPWFKTRHAKRRVVQPELVQDIANYLAVGGVVFLQSDMEFVAVEMCDRFAANPAFK
EECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCHH
KVGTGEWLTENPLPVATERETTTQNRGEPVYRALFERSS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MISGVSALPFVRVRQHVNPLAQKYLTPANPLEWEKAYSTPHQPLHLDIGCARGRFVLQMA
CCCCCCCCHHHHHHHHHCHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEECCCCCEEEEEE
QVEPNWNFLGLEIRESLVIEANQFRSQLGLTNLHYLYCNANNSLQPLLSSLPTGILQRVT
EECCCCCEEEHEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHEE
IQFPDPWFKTRHAKRRVVQPELVQDIANYLAVGGVVFLQSDMEFVAVEMCDRFAANPAFK
EECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCHH
KVGTGEWLTENPLPVATERETTTQNRGEPVYRALFERSS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840