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The map label for this gene is queA [H]

Identifier: 17229290

GI number: 17229290

Start: 2159068

End: 2160261

Strand: Direct

Name: queA [H]

Synonym: alr1798

Alternate gene names: 17229290

Gene position: 2159068-2160261 (Clockwise)

Preceding gene: 17229288

Following gene: 17229291

Centisome position: 33.66

GC content: 43.47

Gene sequence:

>1194_bases
ATGAAGAAACAGATATTACAATTAAACCTTGAACAAACTTCTAGTGCAGCAGCAGAGGATACAAATTTAGATTGTTCATT
GGCTGGGTATGATTACGTACTACCGCCAGAAAGAATTGCCCAAAATCCGGCAGTTCCCAGAGACAGTTCTCGTCTACTAG
TCGTCAATTCTCAAACAACAGGTAAAGAAACACCACCTCTACATCAAATTTTTCGTGATTTACCGGATATTTTGCGTCCT
GGTGACTTATTAATCATGAATAATACGAAGGTCATCCCCGCAAGATTATACGGGCGTAAAAGCTCTGGTGCGGAAGTGGA
AATTTTATTGCTAGAAGAGCGCAAGTTTAACTGTTGGTTGGCTTTAGTTAAACCAGGAAAACGCTTTAAAAAAGGCACAC
AGATTATTTTTGAAGGACTAGGTATGAGGAATTTGGGAATAGAAAATAGTCCCCAATACTCAGTCGCCAGTCCCCACCAG
CTAACCGCTACTGTCTTAGAAACCGACAAAGCCACAGGAGGGCGTTTATTACAGTTTGATTTACCAGAGGGGCAATCTTT
GGTGCAACTTTTAGATAAATTCGGGGAAATACCCCTACCACCCTATATCACTACATCTCAAGCGGCAGATGAACAGTATC
AAACTGTGTATGCCGAGGAACCAGGAGCGATCGCAGCGCCTACGGCTGGGTTACACTTTACTCCAGAGTTATTACAAAAA
TTACGCGATCGCAATATCAATCAAGCTTTTATTACCCTCCACGTTGGTGTGGGAACCTTTCGCCCTGTGGAAGTGGAAGA
TGTAGCCACTCATCAAATGCACGAGGAATGGATTGAAGTCCCCGCCGCCACAGTGGCACAAATCAAAGCCACCAAAGCAG
CCGGCGGTCGAATTATTGCTGTAGGAACTACAGTAGTCCGTGCCTTAGAAGGAGCAGCCGCATCTGGCAATTTACAAGAA
TTTTGTGGAAAAACCAACTTATTTATCTATCCTGGCTATAAATGGCAGGTTGTCGAAGGTTTAATCACCAATTTTCACTT
GCCACGCTCTAGTTTATTAATGCTAGTAAGTGCGCTAATTGGCAGAGAACGCTTGTTAAATATATACCAGGAAGCGATCG
CCTCTCAATATCGTTTCTACTCTTTTGGTGATGCCATGTTGATTCTACCGGAAGCCAGAGGAGTCGAGCAATAG

Upstream 100 bases:

>100_bases
AAGTGTTACGCCATAATGTCAATCTTATAATTTAGGTCTAAAATTGACTACTTTTGTCAAAAAAATGTAAACAAACTTAT
TCCAAGCATCGGGAATTTCA

Downstream 100 bases:

>100_bases
AAGAGAGTTTGCATACAACCTCTGTAAATTAGTTACCCCTGCCGCCAAAATTTTTTAATTTTGAACTTTTAATTTTTCAT
GTTGTAGTGGGTACTCTTAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKKQILQLNLEQTSSAAAEDTNLDCSLAGYDYVLPPERIAQNPAVPRDSSRLLVVNSQTTGKETPPLHQIFRDLPDILRP
GDLLIMNNTKVIPARLYGRKSSGAEVEILLLEERKFNCWLALVKPGKRFKKGTQIIFEGLGMRNLGIENSPQYSVASPHQ
LTATVLETDKATGGRLLQFDLPEGQSLVQLLDKFGEIPLPPYITTSQAADEQYQTVYAEEPGAIAAPTAGLHFTPELLQK
LRDRNINQAFITLHVGVGTFRPVEVEDVATHQMHEEWIEVPAATVAQIKATKAAGGRIIAVGTTVVRALEGAAASGNLQE
FCGKTNLFIYPGYKWQVVEGLITNFHLPRSSLLMLVSALIGRERLLNIYQEAIASQYRFYSFGDAMLILPEARGVEQ

Sequences:

>Translated_397_residues
MKKQILQLNLEQTSSAAAEDTNLDCSLAGYDYVLPPERIAQNPAVPRDSSRLLVVNSQTTGKETPPLHQIFRDLPDILRP
GDLLIMNNTKVIPARLYGRKSSGAEVEILLLEERKFNCWLALVKPGKRFKKGTQIIFEGLGMRNLGIENSPQYSVASPHQ
LTATVLETDKATGGRLLQFDLPEGQSLVQLLDKFGEIPLPPYITTSQAADEQYQTVYAEEPGAIAAPTAGLHFTPELLQK
LRDRNINQAFITLHVGVGTFRPVEVEDVATHQMHEEWIEVPAATVAQIKATKAAGGRIIAVGTTVVRALEGAAASGNLQE
FCGKTNLFIYPGYKWQVVEGLITNFHLPRSSLLMLVSALIGRERLLNIYQEAIASQYRFYSFGDAMLILPEARGVEQ
>Mature_397_residues
MKKQILQLNLEQTSSAAAEDTNLDCSLAGYDYVLPPERIAQNPAVPRDSSRLLVVNSQTTGKETPPLHQIFRDLPDILRP
GDLLIMNNTKVIPARLYGRKSSGAEVEILLLEERKFNCWLALVKPGKRFKKGTQIIFEGLGMRNLGIENSPQYSVASPHQ
LTATVLETDKATGGRLLQFDLPEGQSLVQLLDKFGEIPLPPYITTSQAADEQYQTVYAEEPGAIAAPTAGLHFTPELLQK
LRDRNINQAFITLHVGVGTFRPVEVEDVATHQMHEEWIEVPAATVAQIKATKAAGGRIIAVGTTVVRALEGAAASGNLQE
FCGKTNLFIYPGYKWQVVEGLITNFHLPRSSLLMLVSALIGRERLLNIYQEAIASQYRFYSFGDAMLILPEARGVEQ

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=367, Percent_Identity=43.5967302452316, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 43790; Mature: 43790

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQILQLNLEQTSSAAAEDTNLDCSLAGYDYVLPPERIAQNPAVPRDSSRLLVVNSQTT
CCCCEEEEECHHCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCC
GKETPPLHQIFRDLPDILRPGDLLIMNNTKVIPARLYGRKSSGAEVEILLLEERKFNCWL
CCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEHHEECCCCCCCEEEEEEEECCCCEEEE
ALVKPGKRFKKGTQIIFEGLGMRNLGIENSPQYSVASPHQLTATVLETDKATGGRLLQFD
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEE
LPEGQSLVQLLDKFGEIPLPPYITTSQAADEQYQTVYAEEPGAIAAPTAGLHFTPELLQK
CCCCHHHHHHHHHHCCCCCCCCEECCCCCCCHHHEEEECCCCCEECCCCCCCCCHHHHHH
LRDRNINQAFITLHVGVGTFRPVEVEDVATHQMHEEWIEVPAATVAQIKATKAAGGRIIA
HHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
VGTTVVRALEGAAASGNLQEFCGKTNLFIYPGYKWQVVEGLITNFHLPRSSLLMLVSALI
EHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
GRERLLNIYQEAIASQYRFYSFGDAMLILPEARGVEQ
HHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MKKQILQLNLEQTSSAAAEDTNLDCSLAGYDYVLPPERIAQNPAVPRDSSRLLVVNSQTT
CCCCEEEEECHHCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCC
GKETPPLHQIFRDLPDILRPGDLLIMNNTKVIPARLYGRKSSGAEVEILLLEERKFNCWL
CCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEHHEECCCCCCCEEEEEEEECCCCEEEE
ALVKPGKRFKKGTQIIFEGLGMRNLGIENSPQYSVASPHQLTATVLETDKATGGRLLQFD
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEE
LPEGQSLVQLLDKFGEIPLPPYITTSQAADEQYQTVYAEEPGAIAAPTAGLHFTPELLQK
CCCCHHHHHHHHHHCCCCCCCCEECCCCCCCHHHEEEECCCCCEECCCCCCCCCHHHHHH
LRDRNINQAFITLHVGVGTFRPVEVEDVATHQMHEEWIEVPAATVAQIKATKAAGGRIIA
HHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
VGTTVVRALEGAAASGNLQEFCGKTNLFIYPGYKWQVVEGLITNFHLPRSSLLMLVSALI
EHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
GRERLLNIYQEAIASQYRFYSFGDAMLILPEARGVEQ
HHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA