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The map label for this gene is hupR1 [H]

Identifier: 17229195

GI number: 17229195

Start: 2042268

End: 2043941

Strand: Reverse

Name: hupR1 [H]

Synonym: all1703

Alternate gene names: 17229195

Gene position: 2043941-2042268 (Counterclockwise)

Preceding gene: 17229196

Following gene: 17229194

Centisome position: 31.87

GC content: 41.76

Gene sequence:

>1674_bases
ATGACGGAAACTAAGGTAGAAAAACTGAAGCTCATGGTAGTAGACGATGAGCCAGATAATTTAGATTTACTCTACCGTAC
TTTTAGGCGAGACTTTCAAGTATATAAAGCTAATCATGCCCGGAAAGCTTTGGAAATTCTAGCTCAAGAAGGCGAAATGG
CTGTAATTATTTCAGATCAAAGAATGCCTGAAATGAACGGTACCGAATTTCTCAGCCTGACAGTAGAAAAATTTCCCGAC
ACCATTCGGATTTTACTTACTGGTTTTACAGATGTGGAAGATTTGGTAGAAGCCATTAACTCTGGTCAGGTTTTCAAATA
CATCACCAAACCTTGGAACCCTGAAAGGCTAAAAAATCTAGTTGAGCAAGCTACTGATACATATCGGTTGGTCAAAAAGC
GCACCCACGAATTGCGTCGGTCTTTGCGGCGGGAATCCTTATTTAACGCCGTCACAACAGCAATTCGAGAGTCCTTAGAT
TATGAGAGTATGCTGCAAAAAATCGCAGCTACCATTGGACAAACCTTTGAAGCTACTTGTTGTTTACTCAAACCAGTAGA
AAACAACCGCCTCACTGCAAATCAGTTTTATTATCAAAATCCAGCATACAACTTACCTGATAGCGCCTTTGATCCTGCTA
CTTTAATTTCCCAAGTACTCGAAACCGGCCATTATCAAATTGCCCAAGAGATATACAATGACAATCCCTGTAACTACTTG
GTTGTCCCCCTATCTTACCAACAGCACTTGTTAGCTGTCTTGGCTCTTTACCAGTGGGGACGAGAGCATCCTTGGCAAGA
CGAAGATATTCAATTAATTACGGGTGTGGCTGAACAAGCAGCTTTAGCCGTTTCCCAAGCAAAACTCTACCAACGCCTGC
AAGAAAAACAACAGCAAATTAGTGCTGAATTAGAAGTGGCGCGGCAAATTCAAAATAATTTACTACGCCAAACCCTACCG
GATATAAAAAGCGCAAAATTACAAGCTTGCTGCTATCCTGCAAGAGAAGTAGGAGGCGATTTTTTTGAAATCTTTGTTCA
CCCCACCAAAGGTGATGTGTGGTTAGCAGTCGGTGATGTTTCCGGTAAAGGCGTACCTGCTGCTTTATTCATGGCTAGTG
CAATTTCTGTTTTAAGACGGGAATTAGCCCAAGAAATCCCAGCCGAACCCAATGTAGTTATACACAATCTCAATCATGCC
TTATGTGATGACTTAATTAGTAACAATTGCTTTATTACTTTTGTGCTAGCTCGTTATACACCTACTACTAGAGAGTTAGT
CTATGCAAATGCTGGACATATTTATCCCCTGGTTTGGTCAAATCAAATTGCCCCAGAAAAACCTAACTATCTTACCGTGA
GAGGCATTCCTTTAGGCATTTTACCGAAATGGCAGGCTAAGTCGGGTAGTTTGATTCTGGCCCCCGGAGATACACTACTG
CTGGCCAGTGATGGTATCACGGAAGCAATGGTATCAAATAGCTTATTGAAACAAGTAAAAACCGTGGGTGGCGTTCAGCT
AGCAAGTCATTCTATGCTGAATCAAGATGGTCTGTGGCAACTCTTACAACAAGAGCCTCAACCACTATCTCTCAACCATT
TACTATCACGGATTCAGGCAGATAACCACATTCAAGAAGATGATCAAACCATACTCTCACTGGAGGTTTTGTAA

Upstream 100 bases:

>100_bases
TTTCATCAAAGATTACTTTTGAGAACGCCTTTTGACGACTCAGTGAGATAACAGTTGCCATATCTCGCTAATTTTTAGTA
TCCGAGAAAGCAAGTCAAAT

Downstream 100 bases:

>100_bases
GTTATGAAAAGTGAGCTTCACGTACCAAGCGACTTGAACTATTTAAACATAGTGGAACACTGGTTATTGGGATGCTTGAA
AATCCAATTAGGAGAATCTG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTETKVEKLKLMVVDDEPDNLDLLYRTFRRDFQVYKANHARKALEILAQEGEMAVIISDQRMPEMNGTEFLSLTVEKFPD
TIRILLTGFTDVEDLVEAINSGQVFKYITKPWNPERLKNLVEQATDTYRLVKKRTHELRRSLRRESLFNAVTTAIRESLD
YESMLQKIAATIGQTFEATCCLLKPVENNRLTANQFYYQNPAYNLPDSAFDPATLISQVLETGHYQIAQEIYNDNPCNYL
VVPLSYQQHLLAVLALYQWGREHPWQDEDIQLITGVAEQAALAVSQAKLYQRLQEKQQQISAELEVARQIQNNLLRQTLP
DIKSAKLQACCYPAREVGGDFFEIFVHPTKGDVWLAVGDVSGKGVPAALFMASAISVLRRELAQEIPAEPNVVIHNLNHA
LCDDLISNNCFITFVLARYTPTTRELVYANAGHIYPLVWSNQIAPEKPNYLTVRGIPLGILPKWQAKSGSLILAPGDTLL
LASDGITEAMVSNSLLKQVKTVGGVQLASHSMLNQDGLWQLLQQEPQPLSLNHLLSRIQADNHIQEDDQTILSLEVL

Sequences:

>Translated_557_residues
MTETKVEKLKLMVVDDEPDNLDLLYRTFRRDFQVYKANHARKALEILAQEGEMAVIISDQRMPEMNGTEFLSLTVEKFPD
TIRILLTGFTDVEDLVEAINSGQVFKYITKPWNPERLKNLVEQATDTYRLVKKRTHELRRSLRRESLFNAVTTAIRESLD
YESMLQKIAATIGQTFEATCCLLKPVENNRLTANQFYYQNPAYNLPDSAFDPATLISQVLETGHYQIAQEIYNDNPCNYL
VVPLSYQQHLLAVLALYQWGREHPWQDEDIQLITGVAEQAALAVSQAKLYQRLQEKQQQISAELEVARQIQNNLLRQTLP
DIKSAKLQACCYPAREVGGDFFEIFVHPTKGDVWLAVGDVSGKGVPAALFMASAISVLRRELAQEIPAEPNVVIHNLNHA
LCDDLISNNCFITFVLARYTPTTRELVYANAGHIYPLVWSNQIAPEKPNYLTVRGIPLGILPKWQAKSGSLILAPGDTLL
LASDGITEAMVSNSLLKQVKTVGGVQLASHSMLNQDGLWQLLQQEPQPLSLNHLLSRIQADNHIQEDDQTILSLEVL
>Mature_556_residues
TETKVEKLKLMVVDDEPDNLDLLYRTFRRDFQVYKANHARKALEILAQEGEMAVIISDQRMPEMNGTEFLSLTVEKFPDT
IRILLTGFTDVEDLVEAINSGQVFKYITKPWNPERLKNLVEQATDTYRLVKKRTHELRRSLRRESLFNAVTTAIRESLDY
ESMLQKIAATIGQTFEATCCLLKPVENNRLTANQFYYQNPAYNLPDSAFDPATLISQVLETGHYQIAQEIYNDNPCNYLV
VPLSYQQHLLAVLALYQWGREHPWQDEDIQLITGVAEQAALAVSQAKLYQRLQEKQQQISAELEVARQIQNNLLRQTLPD
IKSAKLQACCYPAREVGGDFFEIFVHPTKGDVWLAVGDVSGKGVPAALFMASAISVLRRELAQEIPAEPNVVIHNLNHAL
CDDLISNNCFITFVLARYTPTTRELVYANAGHIYPLVWSNQIAPEKPNYLTVRGIPLGILPKWQAKSGSLILAPGDTLLL
ASDGITEAMVSNSLLKQVKTVGGVQLASHSMLNQDGLWQLLQQEPQPLSLNHLLSRIQADNHIQEDDQTILSLEVL

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=32.03125, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62865; Mature: 62733

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETKVEKLKLMVVDDEPDNLDLLYRTFRRDFQVYKANHARKALEILAQEGEMAVIISDQ
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
RMPEMNGTEFLSLTVEKFPDTIRILLTGFTDVEDLVEAINSGQVFKYITKPWNPERLKNL
CCCCCCCCEEEEEEHHHCCCEEEHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHH
VEQATDTYRLVKKRTHELRRSLRRESLFNAVTTAIRESLDYESMLQKIAATIGQTFEATC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHE
CLLKPVENNRLTANQFYYQNPAYNLPDSAFDPATLISQVLETGHYQIAQEIYNDNPCNYL
EEEECCCCCCEEECCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEE
VVPLSYQQHLLAVLALYQWGREHPWQDEDIQLITGVAEQAALAVSQAKLYQRLQEKQQQI
EEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAELEVARQIQNNLLRQTLPDIKSAKLQACCYPAREVGGDFFEIFVHPTKGDVWLAVGDV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCEEEEEECC
SGKGVPAALFMASAISVLRRELAQEIPAEPNVVIHNLNHALCDDLISNNCFITFVLARYT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEECCC
PTTRELVYANAGHIYPLVWSNQIAPEKPNYLTVRGIPLGILPKWQAKSGSLILAPGDTLL
CCCHHEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCEEE
LASDGITEAMVSNSLLKQVKTVGGVQLASHSMLNQDGLWQLLQQEPQPLSLNHLLSRIQA
EECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHC
DNHIQEDDQTILSLEVL
CCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
TETKVEKLKLMVVDDEPDNLDLLYRTFRRDFQVYKANHARKALEILAQEGEMAVIISDQ
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
RMPEMNGTEFLSLTVEKFPDTIRILLTGFTDVEDLVEAINSGQVFKYITKPWNPERLKNL
CCCCCCCCEEEEEEHHHCCCEEEHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHH
VEQATDTYRLVKKRTHELRRSLRRESLFNAVTTAIRESLDYESMLQKIAATIGQTFEATC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHE
CLLKPVENNRLTANQFYYQNPAYNLPDSAFDPATLISQVLETGHYQIAQEIYNDNPCNYL
EEEECCCCCCEEECCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEE
VVPLSYQQHLLAVLALYQWGREHPWQDEDIQLITGVAEQAALAVSQAKLYQRLQEKQQQI
EEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAELEVARQIQNNLLRQTLPDIKSAKLQACCYPAREVGGDFFEIFVHPTKGDVWLAVGDV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCEEEEEECC
SGKGVPAALFMASAISVLRRELAQEIPAEPNVVIHNLNHALCDDLISNNCFITFVLARYT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEECCC
PTTRELVYANAGHIYPLVWSNQIAPEKPNYLTVRGIPLGILPKWQAKSGSLILAPGDTLL
CCCHHEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCEEE
LASDGITEAMVSNSLLKQVKTVGGVQLASHSMLNQDGLWQLLQQEPQPLSLNHLLSRIQA
EECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHC
DNHIQEDDQTILSLEVL
CCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]