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The map label for this gene is truB [H]

Identifier: 17229035

GI number: 17229035

Start: 1803558

End: 1804439

Strand: Direct

Name: truB [H]

Synonym: alr1543

Alternate gene names: 17229035

Gene position: 1803558-1804439 (Clockwise)

Preceding gene: 17229032

Following gene: 17229036

Centisome position: 28.12

GC content: 42.86

Gene sequence:

>882_bases
ATGCAAGGTTTTATTAATTTAAACAAACAATTTGGTTGGACTTCCCACGACTGTGTAGCACGTTTAAGAAAAATGTTACG
TCTCAAACGTGTGGGACACGCGGGAACATTAGATCCAGCAGCCACTGGGGTGTTACCTATCGCAGTTGGCAAAGCTACAC
GTTTATTGCAGTATCTGCCTAGTGACAAAGCTTATAAAGCTACTGTCCGTTTTGGTGTGCAGACTACAACAGATGATTTG
CAAGGTGAAATTATTACTTCCCAACCTTGTCGGGGATTGAGTTTGTCTGAGGTGAAAACTGCACTACCCCAATTTATCGG
CAAGATTGAACAAATACCACCCATTTACAGTGCGATTCAAGTAGAGGGAAAACGCTTATATGATTTGGCGCGTAAAGGCG
AAATCATAGAAGTGCCAGCACGGACTGTAGAAGTTTTTAGTATAGATGTTTTGGATTGGCGGGAAGGAGATTTTCCCGAA
TTGGATGTGGCGATCGCCTGTGGTAGTGGTACATATATTAGAGCGATCGCTCGTGATTTAGGTGCAGTTTTCCACACAGG
TGGAACTCTCGCCGCTTTGATCCGCACTCACAGCAGTGGCTTTAATCTAACAGATAGTCTCACCTTGACAGACTTAGAAA
CCCAACTGCAAGCAGGGACTTTTGAACCAACTCCCGCCGATGCAGCTTTGCAATGTTTACCGTCAGTTACCTTACCTAGC
ATATCTGCACAAAAATGGTGTCAGGGACAGCGAATTGAATTAAATTTAGAAACTATTGGTAAAGTGCGAGTTTATCAAGC
AGAAACAAATATTTTCTTGGGTATAGGAGAATTGCAAGCAGGTGTGTTAATTCCCCAAATGGTGTTTGAACCGATTTCTT
AA

Upstream 100 bases:

>100_bases
GGGGGTGGAGAACAAAAACACTAACCCCTAGTCTCTAGTTACTTTTTTATTTTTGAATTTTGTAGCTTTAGCTTCCCGTA
GGGTATTTTGAATTGTTACT

Downstream 100 bases:

>100_bases
ATAGGACTTAAGTATGAAAACGAAAGATCAAGGGTTCGGAGAAGAGTATAGGGGTATAGGGGTTTCAAACCCCTACACCC
TTACACCCTTACACCCTTAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQGFINLNKQFGWTSHDCVARLRKMLRLKRVGHAGTLDPAATGVLPIAVGKATRLLQYLPSDKAYKATVRFGVQTTTDDL
QGEIITSQPCRGLSLSEVKTALPQFIGKIEQIPPIYSAIQVEGKRLYDLARKGEIIEVPARTVEVFSIDVLDWREGDFPE
LDVAIACGSGTYIRAIARDLGAVFHTGGTLAALIRTHSSGFNLTDSLTLTDLETQLQAGTFEPTPADAALQCLPSVTLPS
ISAQKWCQGQRIELNLETIGKVRVYQAETNIFLGIGELQAGVLIPQMVFEPIS

Sequences:

>Translated_293_residues
MQGFINLNKQFGWTSHDCVARLRKMLRLKRVGHAGTLDPAATGVLPIAVGKATRLLQYLPSDKAYKATVRFGVQTTTDDL
QGEIITSQPCRGLSLSEVKTALPQFIGKIEQIPPIYSAIQVEGKRLYDLARKGEIIEVPARTVEVFSIDVLDWREGDFPE
LDVAIACGSGTYIRAIARDLGAVFHTGGTLAALIRTHSSGFNLTDSLTLTDLETQLQAGTFEPTPADAALQCLPSVTLPS
ISAQKWCQGQRIELNLETIGKVRVYQAETNIFLGIGELQAGVLIPQMVFEPIS
>Mature_293_residues
MQGFINLNKQFGWTSHDCVARLRKMLRLKRVGHAGTLDPAATGVLPIAVGKATRLLQYLPSDKAYKATVRFGVQTTTDDL
QGEIITSQPCRGLSLSEVKTALPQFIGKIEQIPPIYSAIQVEGKRLYDLARKGEIIEVPARTVEVFSIDVLDWREGDFPE
LDVAIACGSGTYIRAIARDLGAVFHTGGTLAALIRTHSSGFNLTDSLTLTDLETQLQAGTFEPTPADAALQCLPSVTLPS
ISAQKWCQGQRIELNLETIGKVRVYQAETNIFLGIGELQAGVLIPQMVFEPIS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=241, Percent_Identity=31.5352697095436, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI215599015, Length=215, Percent_Identity=31.1627906976744, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503337, Length=215, Percent_Identity=31.1627906976744, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=293, Percent_Identity=35.4948805460751, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=28, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=146, Percent_Identity=37.6712328767123, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=215, Percent_Identity=29.3023255813954, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31908; Mature: 31908

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFINLNKQFGWTSHDCVARLRKMLRLKRVGHAGTLDPAATGVLPIAVGKATRLLQYLP
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHHCC
SDKAYKATVRFGVQTTTDDLQGEIITSQPCRGLSLSEVKTALPQFIGKIEQIPPIYSAIQ
CCCCEEEEEEECCEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHE
VEGKRLYDLARKGEIIEVPARTVEVFSIDVLDWREGDFPELDVAIACGSGTYIRAIARDL
ECCHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GAVFHTGGTLAALIRTHSSGFNLTDSLTLTDLETQLQAGTFEPTPADAALQCLPSVTLPS
HHHHHCCCHHEEHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCHHHHHHHCCCCCCCC
ISAQKWCQGQRIELNLETIGKVRVYQAETNIFLGIGELQAGVLIPQMVFEPIS
CCHHHHCCCCEEEEEHEECCEEEEEEECCCEEEEECCCCCCEECHHHHHCCCC
>Mature Secondary Structure
MQGFINLNKQFGWTSHDCVARLRKMLRLKRVGHAGTLDPAATGVLPIAVGKATRLLQYLP
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHHCC
SDKAYKATVRFGVQTTTDDLQGEIITSQPCRGLSLSEVKTALPQFIGKIEQIPPIYSAIQ
CCCCEEEEEEECCEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHE
VEGKRLYDLARKGEIIEVPARTVEVFSIDVLDWREGDFPELDVAIACGSGTYIRAIARDL
ECCHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GAVFHTGGTLAALIRTHSSGFNLTDSLTLTDLETQLQAGTFEPTPADAALQCLPSVTLPS
HHHHHCCCHHEEHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCHHHHHHHCCCCCCCC
ISAQKWCQGQRIELNLETIGKVRVYQAETNIFLGIGELQAGVLIPQMVFEPIS
CCHHHHCCCCEEEEEHEECCEEEEEEECCCEEEEECCCCCCEECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA