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The map label for this gene is cobS [H]

Identifier: 17227875

GI number: 17227875

Start: 442487

End: 443245

Strand: Direct

Name: cobS [H]

Synonym: alr0379

Alternate gene names: 17227875

Gene position: 442487-443245 (Clockwise)

Preceding gene: 17227873

Following gene: 17227877

Centisome position: 6.9

GC content: 45.06

Gene sequence:

>759_bases
ATGGTGAAGCTGTTGTTATTGAATTTATTGGCAAGTATTATATTTTACACAAATATCCCTCTGCCTTATATTAATGGCTT
GGATTTTCAACGAGTGGCGCGGCTAGTCCCTACGGTGGGGTTAATTATTGGGGGGATTTTAGGGTTATTCGATGGAGGCA
TGAATTATCTGGGTATGCCCGTATTAACTCGTAGTGCCTTGGTGGTGGTACTGTGGATTTTTATCACTGGGGGGTTACAT
TTAGATGGGGCGATGGATACGGCTGATGGTTTGGCTGTGGGTGACCCAGAACGGCGGTTGGAAGTGATGGCGGATAGTGC
TACAGGTGCATTTGGAGCTATGAGTGCGATCGCTATATTATTACTAAAAACCTCTGCCCTCACAGAGATTGGGGAATACC
GTTGGCTAGTATTAATGGCTGCTTGTGGTTGGGGACGTTGGGGACAACAAGTGGCGATCGCCTGTTATCCTTATTTAAAA
GCCACTGGTAAAGGCGCATTCCATAAACAAGCTATTCGCTCATACAAAGATTTATTACCCGGATTATGCTTGATGTTTGC
AATCAGTGGCTTATTTTGGCTAGTAAATAACCATCATCTATTGATATCAGTAGTGGTGTTAATTGCCGGGAGTGCGATCG
CTACTTTAACAGCTGCATGGTTTAATCACAAATTAGGCGGACACACCGGCGACACCTACGGCGCAGTCGTCGAGTGGACA
GAGGCTTTATTTCTCTGCGTACTCACCATACTCACATAA

Upstream 100 bases:

>100_bases
AACATGGTCAAAAAGTATACATAAGAATTTGCTTTTTTGTTCAGTTTTGGGGGAAATTGAAACGCGGAGGATTGGTTATG
GGTGATGAAGTGGGAATGAG

Downstream 100 bases:

>100_bases
CTTACCATGTAGGCAATAATTCTATCTGTGTTTATCTGCGTGCATCTGCGTTTAATTTCATCCCAGTTTACTCATCAGCA
CAAACATAATGCGTTCATTC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MVKLLLLNLLASIIFYTNIPLPYINGLDFQRVARLVPTVGLIIGGILGLFDGGMNYLGMPVLTRSALVVVLWIFITGGLH
LDGAMDTADGLAVGDPERRLEVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMFAISGLFWLVNNHHLLISVVVLIAGSAIATLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT

Sequences:

>Translated_252_residues
MVKLLLLNLLASIIFYTNIPLPYINGLDFQRVARLVPTVGLIIGGILGLFDGGMNYLGMPVLTRSALVVVLWIFITGGLH
LDGAMDTADGLAVGDPERRLEVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMFAISGLFWLVNNHHLLISVVVLIAGSAIATLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT
>Mature_252_residues
MVKLLLLNLLASIIFYTNIPLPYINGLDFQRVARLVPTVGLIIGGILGLFDGGMNYLGMPVLTRSALVVVLWIFITGGLH
LDGAMDTADGLAVGDPERRLEVMADSATGAFGAMSAIAILLLKTSALTEIGEYRWLVLMAACGWGRWGQQVAIACYPYLK
ATGKGAFHKQAIRSYKDLLPGLCLMFAISGLFWLVNNHHLLISVVVLIAGSAIATLTAAWFNHKLGGHTGDTYGAVVEWT
EALFLCVLTILT

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=31.0756972111554, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27108; Mature: 27108

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHC
>Mature Secondary Structure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HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA