Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 17227785

GI number: 17227785

Start: 325649

End: 326836

Strand: Direct

Name: rihA [H]

Synonym: alr0289

Alternate gene names: 17227785

Gene position: 325649-326836 (Clockwise)

Preceding gene: 17227784

Following gene: 17227786

Centisome position: 5.08

GC content: 45.2

Gene sequence:

>1188_bases
ATGCTCAAAATTCCTAATGTGCAGAAGTTATTTTCTGCTGCTACATTTTTAGTATCGATCACAACGATTTTTTGTAGCCA
ACCTGCACTTGCAGCCTCTTTTAAGCCAACACCCCTAATTATTGACGACGATGGCAGCCAAGACGGCATGACTGCATTGG
CTTATATGCTAGCCAATCCCAAATTTGACATTCAGGCAATTACCATCGCCCAAGGTATTGCCCGCCCACAAAGTTTTGCG
AACAACCTGGAACGAATGCTAGGCAGACTAGATATCTCTGGTATCCCTATTGGCATTGGCAGATCAACTCCCCTGGCAGG
AAACAATACTTTTCCAGAACCTATTCGTGCTGGTGCAGACACTTTTTGGTCTCCCTTCGTCCAACTACCTAATACAGCAC
CACTGGTGATAACTCGACCAGCCGCAGAACTGATTGTGGAAAAAGTGAAGCGGTCATTAACACCTGTGGCAATCTTGGCA
ACTGGCCCCCTAACCAATATTGCTGAAGCATTACGCCTTGACCCCACAATTATCAACAACATTGCCGTCATCGAAATCAT
GGGAGGTGCAGTTTTCGTACCCGGAAATCTCCCAGTCCTGCCATATCCCCCATTTTCCACCAACACTACAGCTGAATTTA
ACATCTGGGCTGACCCCTTAGCAGCACAAGAAGTATTTGCAGCCGGAGGGCAAGGATTAAAAATTCAGTTGACCCCCCTT
GATGCTACAAACCAGATTGCCTTCTCTCGTGCCGATCAACAAGCATGGCTAGCTACTGCAACACCAGAAAGTAAGTTAGC
AGCAGAATTTTTAGACTTTGCCTTGACAGTAATTCAAAGTAACAATGACCCCAACCCAGCTTGGGATCTAGTTGCAGCCA
TTAACCTGAGTGAACCAGATTTCTCAGTAGAAACTTCCTTATACCTGGAAGTTGATACGACCTCAGATCCTGGAAATACT
CAAGGGCAAACTCGTGCTATTCCTAATTTGCCTCCCAATGTTCTAGTTTCCCTCAACCCCAGTTTTAATAATTTGCCCTT
CCAACCTGGCCAAGTCTTCTCTTACCTACAAATCCAGTCGGTTCCGGAACCAACATTAATCCCAGGAATCTTAGTTCTAG
CTACAGTCAGTGCTGGTATGATGACGCGACGTTCTCAAAAAAAAGTTAATAACAAATTGCGGGCATAA

Upstream 100 bases:

>100_bases
TTATTTTAATTATGTAATTTACGCAGCAATTAAGGTATCATTCTCTAAATAGCTAGATATCAGCCACTTCCTGAATTAAA
AGGCTAGGAACGATATTCTT

Downstream 100 bases:

>100_bases
AGCATAGAAAATTAATTGCTTTTCCATGTCCTATGCTAGCCAAAAATTCAGAATTATCCCTCTGCTATCACTTTGTTTCT
TTCGCCCTTCCCATAAGTGC

Product: hypothetical protein

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MLKIPNVQKLFSAATFLVSITTIFCSQPALAASFKPTPLIIDDDGSQDGMTALAYMLANPKFDIQAITIAQGIARPQSFA
NNLERMLGRLDISGIPIGIGRSTPLAGNNTFPEPIRAGADTFWSPFVQLPNTAPLVITRPAAELIVEKVKRSLTPVAILA
TGPLTNIAEALRLDPTIINNIAVIEIMGGAVFVPGNLPVLPYPPFSTNTTAEFNIWADPLAAQEVFAAGGQGLKIQLTPL
DATNQIAFSRADQQAWLATATPESKLAAEFLDFALTVIQSNNDPNPAWDLVAAINLSEPDFSVETSLYLEVDTTSDPGNT
QGQTRAIPNLPPNVLVSLNPSFNNLPFQPGQVFSYLQIQSVPEPTLIPGILVLATVSAGMMTRRSQKKVNNKLRA

Sequences:

>Translated_395_residues
MLKIPNVQKLFSAATFLVSITTIFCSQPALAASFKPTPLIIDDDGSQDGMTALAYMLANPKFDIQAITIAQGIARPQSFA
NNLERMLGRLDISGIPIGIGRSTPLAGNNTFPEPIRAGADTFWSPFVQLPNTAPLVITRPAAELIVEKVKRSLTPVAILA
TGPLTNIAEALRLDPTIINNIAVIEIMGGAVFVPGNLPVLPYPPFSTNTTAEFNIWADPLAAQEVFAAGGQGLKIQLTPL
DATNQIAFSRADQQAWLATATPESKLAAEFLDFALTVIQSNNDPNPAWDLVAAINLSEPDFSVETSLYLEVDTTSDPGNT
QGQTRAIPNLPPNVLVSLNPSFNNLPFQPGQVFSYLQIQSVPEPTLIPGILVLATVSAGMMTRRSQKKVNNKLRA
>Mature_395_residues
MLKIPNVQKLFSAATFLVSITTIFCSQPALAASFKPTPLIIDDDGSQDGMTALAYMLANPKFDIQAITIAQGIARPQSFA
NNLERMLGRLDISGIPIGIGRSTPLAGNNTFPEPIRAGADTFWSPFVQLPNTAPLVITRPAAELIVEKVKRSLTPVAILA
TGPLTNIAEALRLDPTIINNIAVIEIMGGAVFVPGNLPVLPYPPFSTNTTAEFNIWADPLAAQEVFAAGGQGLKIQLTPL
DATNQIAFSRADQQAWLATATPESKLAAEFLDFALTVIQSNNDPNPAWDLVAAINLSEPDFSVETSLYLEVDTTSDPGNT
QGQTRAIPNLPPNVLVSLNPSFNNLPFQPGQVFSYLQIQSVPEPTLIPGILVLATVSAGMMTRRSQKKVNNKLRA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=256, Percent_Identity=32.03125, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=26.9736842105263, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786213, Length=310, Percent_Identity=30, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565474, Length=230, Percent_Identity=29.5652173913043, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 42279; Mature: 42279

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIPNVQKLFSAATFLVSITTIFCSQPALAASFKPTPLIIDDDGSQDGMTALAYMLANP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHCCC
KFDIQAITIAQGIARPQSFANNLERMLGRLDISGIPIGIGRSTPLAGNNTFPEPIRAGAD
CCCEEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCH
TFWSPFVQLPNTAPLVITRPAAELIVEKVKRSLTPVAILATGPLTNIAEALRLDPTIINN
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHCC
IAVIEIMGGAVFVPGNLPVLPYPPFSTNTTAEFNIWADPLAAQEVFAAGGQGLKIQLTPL
EEEEEEECCEEECCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEEC
DATNQIAFSRADQQAWLATATPESKLAAEFLDFALTVIQSNNDPNPAWDLVAAINLSEPD
CCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCC
FSVETSLYLEVDTTSDPGNTQGQTRAIPNLPPNVLVSLNPSFNNLPFQPGQVFSYLQIQS
CCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHEEEEEECC
VPEPTLIPGILVLATVSAGMMTRRSQKKVNNKLRA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKIPNVQKLFSAATFLVSITTIFCSQPALAASFKPTPLIIDDDGSQDGMTALAYMLANP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHCCC
KFDIQAITIAQGIARPQSFANNLERMLGRLDISGIPIGIGRSTPLAGNNTFPEPIRAGAD
CCCEEEEEEHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHCCCH
TFWSPFVQLPNTAPLVITRPAAELIVEKVKRSLTPVAILATGPLTNIAEALRLDPTIINN
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHCC
IAVIEIMGGAVFVPGNLPVLPYPPFSTNTTAEFNIWADPLAAQEVFAAGGQGLKIQLTPL
EEEEEEECCEEECCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEEC
DATNQIAFSRADQQAWLATATPESKLAAEFLDFALTVIQSNNDPNPAWDLVAAINLSEPD
CCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEECCCCC
FSVETSLYLEVDTTSDPGNTQGQTRAIPNLPPNVLVSLNPSFNNLPFQPGQVFSYLQIQS
CCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHEEEEEECC
VPEPTLIPGILVLATVSAGMMTRRSQKKVNNKLRA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA