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The map label for this gene is sigB [H]

Identifier: 17227773

GI number: 17227773

Start: 306021

End: 306806

Strand: Direct

Name: sigB [H]

Synonym: alr0277

Alternate gene names: 17227773

Gene position: 306021-306806 (Clockwise)

Preceding gene: 17227772

Following gene: 17227775

Centisome position: 4.77

GC content: 40.71

Gene sequence:

>786_bases
ATGGCAGCAAGTGAGTCCTCTATGCAAACTGATGGTATGGAGTTATTGCGCTCATACCACCATAATCCTTCTATTAAACT
TCGTAACAGACTGGTTCAATTACACACCGGCTTAGTAAGAAAAATGGCTCATAAATTTAGCCATCAATGTAATGAACCTT
ATGAAGATTTGGAACAAATTGGTTATTTTGGTTTAATCAGAGCTATAGAACGTTTTGATCCCAGTCAAGGGTATGCTTTT
AGTTCCTTTGCTGTGCCATATATTAGAGGTGAAATGCTGCATTTTCTCCGCGATCGCAGTACGCTATTAAAAATTCCTCG
TCGTTGGCAAGAATTATATAACGAAGGACAAAAAGTCCGTAAAGAATTATCAATATCTCTAGGTCGTCCGCCCAAGGATG
CTGAAATTGCCAAGTACCTCAAGGTATCTGTGCAAGAATGGCAAGAAACTAAGTTGGCTGCCCAAAATCGGATGCCTTTA
AGTTTAGATGCTACGGCAGTACACTATGTAGATTGCCAAATTACCTTAGGTGAAGCCCTTCCTTGTCCTCGAACAATTGC
CCAACAACAACAGGATGAAGAACGGCAACAACTGCAAGGGGCAATTAGTATGCTAGAGGAAAAGCCCCGCCAAGCCGTGG
AAATGGTCTTCTTGAAAGAACTTTCTCGCAAAGATGCTGCTAAAAACATCGGTACTAGTCCTATGACGGTAACGAGGTAT
TTGCAAAAGGGTATTCAAGATTTAATTTGTTATTTACAACCACAAGTAATGCCTACTGGTTCATAG

Upstream 100 bases:

>100_bases
TTTATTCAGGCGCTACTAAGGAAAATAATGACAGCTTTGAAGAACATATTACGCAACTCCAATTTGGTGAAAAAAGGAGT
TACCACAAGGGAATTTTTAT

Downstream 100 bases:

>100_bases
TCAAAAGCCCATAGTCCAGAGTTTATTGACTCACGACTAATGACTATTGACCAGTCACTATCTACCGAGATTTTAAATCA
GCGATCGCCCCTTTAGTCAT

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAASESSMQTDGMELLRSYHHNPSIKLRNRLVQLHTGLVRKMAHKFSHQCNEPYEDLEQIGYFGLIRAIERFDPSQGYAF
SSFAVPYIRGEMLHFLRDRSTLLKIPRRWQELYNEGQKVRKELSISLGRPPKDAEIAKYLKVSVQEWQETKLAAQNRMPL
SLDATAVHYVDCQITLGEALPCPRTIAQQQQDEERQQLQGAISMLEEKPRQAVEMVFLKELSRKDAAKNIGTSPMTVTRY
LQKGIQDLICYLQPQVMPTGS

Sequences:

>Translated_261_residues
MAASESSMQTDGMELLRSYHHNPSIKLRNRLVQLHTGLVRKMAHKFSHQCNEPYEDLEQIGYFGLIRAIERFDPSQGYAF
SSFAVPYIRGEMLHFLRDRSTLLKIPRRWQELYNEGQKVRKELSISLGRPPKDAEIAKYLKVSVQEWQETKLAAQNRMPL
SLDATAVHYVDCQITLGEALPCPRTIAQQQQDEERQQLQGAISMLEEKPRQAVEMVFLKELSRKDAAKNIGTSPMTVTRY
LQKGIQDLICYLQPQVMPTGS
>Mature_260_residues
AASESSMQTDGMELLRSYHHNPSIKLRNRLVQLHTGLVRKMAHKFSHQCNEPYEDLEQIGYFGLIRAIERFDPSQGYAFS
SFAVPYIRGEMLHFLRDRSTLLKIPRRWQELYNEGQKVRKELSISLGRPPKDAEIAKYLKVSVQEWQETKLAAQNRMPLS
LDATAVHYVDCQITLGEALPCPRTIAQQQQDEERQQLQGAISMLEEKPRQAVEMVFLKELSRKDAAKNIGTSPMTVTRYL
QKGIQDLICYLQPQVMPTGS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=184, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29995; Mature: 29864

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASESSMQTDGMELLRSYHHNPSIKLRNRLVQLHTGLVRKMAHKFSHQCNEPYEDLEQI
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GYFGLIRAIERFDPSQGYAFSSFAVPYIRGEMLHFLRDRSTLLKIPRRWQELYNEGQKVR
HHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELSISLGRPPKDAEIAKYLKVSVQEWQETKLAAQNRMPLSLDATAVHYVDCQITLGEAL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEEECCCCC
PCPRTIAQQQQDEERQQLQGAISMLEEKPRQAVEMVFLKELSRKDAAKNIGTSPMTVTRY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQKGIQDLICYLQPQVMPTGS
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AASESSMQTDGMELLRSYHHNPSIKLRNRLVQLHTGLVRKMAHKFSHQCNEPYEDLEQI
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GYFGLIRAIERFDPSQGYAFSSFAVPYIRGEMLHFLRDRSTLLKIPRRWQELYNEGQKVR
HHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELSISLGRPPKDAEIAKYLKVSVQEWQETKLAAQNRMPLSLDATAVHYVDCQITLGEAL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEEECCCCC
PCPRTIAQQQQDEERQQLQGAISMLEEKPRQAVEMVFLKELSRKDAAKNIGTSPMTVTRY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQKGIQDLICYLQPQVMPTGS
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]