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The map label for this gene is ddh [H]

Identifier: 17227554

GI number: 17227554

Start: 62394

End: 63419

Strand: Direct

Name: ddh [H]

Synonym: alr0058

Alternate gene names: 17227554

Gene position: 62394-63419 (Clockwise)

Preceding gene: 17227553

Following gene: 17227558

Centisome position: 0.97

GC content: 42.5

Gene sequence:

>1026_bases
ATGAAAGTAGCAGTCTTCAGTACAAAAGCCTATGATCGGCAGTTTTTAGAAGCTGCAAATGCTCCAAAGCATCATGAGTT
GGCATTCTTTGAACCCCGTCTCAATCAAGATACCGCCATTTTAGCCGCCGGATTTCCGGCGGTTTGCGTATTTGTACATG
ATCAAGTTGATGCAGCTACTTTAGAAATTCTTGCTTCACGAGGAACTCGCTTGATTGTCCTCCGTTGTGCAGGTTTCAAT
AATGTAGACTTAAAAGCTGCTAATAAGTTAGGCGTTACTGTAGTGCGTGTTCCCGCCTATTCACCCTATGGGGTAGCCGA
ACACGCAGTGGGTTTGATTTTGAGCCTCAATCGCAAAATTCACCGTGCTTATAACCGCGTCCGCGAAGGTAATTTCGCTT
TAGATGGACTTTTAGGCTTTAACATCAATGGACGCACGGTGGGAATTATCGGTACTGGCAAAATCGGGTTGATTTTGGGA
CAAATTATGAAAGGGTTTGGCTGTCGCCTACTCGCCTATGATGTTTACCGCAACCCAGAGATGGAATCATTAGGCGGCGA
ATATGTAGAATTACCAGAACTATTTGCCAATTCTGATATTATTTCTCTCCATTGTCCCCTGATGCCCCAAACTCATCACT
TAATCAACGCGGAAGCAATAGAGCAGGTAAAACCAGGGGTAATGTTAATCAATACCAGCAGAGGTGCGCTTATTCATACC
CAAGCTGTAATTGAAGGACTCAAGACTGGCAAAATCGGTTCTTTGGGTGTAGATGTTTATGAGCAGGAATCAGAATTGTT
TTTTGAGGATTTATCAGGGGAAATCATTCAAGATGATATCTTCCAAAGACTGACAACATTTCCTAATGTACTTATCACAG
GACACCAAGCATTTTTTACAGAAGACGCTTTACGCAACATAGCAGAGACAACCTTAAACAACATTGCTGATATTGAGCAA
GGTCGTTCTTGTCCCAATGAAATCCGTTATCAACCAGAAGTAGAAGCTAAGGTTTTAGTTAGTTGA

Upstream 100 bases:

>100_bases
ATAATAGACATTTTCAGCTAGCCCTTAGTGAACATCGCCTGTTCAAACACCAAGCTAATATGTATGTGAAAAATATCCGG
CATACAGCCTGAGGTTTGGC

Downstream 100 bases:

>100_bases
GTATAGGGGTGTGGGGGTTTAGGGGTAGAAATTTTGAAAACCCTTACACTCTTACACCCTTATCCCCCTACACCCTTACA
CCCTTACACCTAGTCTCAAC

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVAVFSTKAYDRQFLEAANAPKHHELAFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVTVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYRNPEMESLGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS

Sequences:

>Translated_341_residues
MKVAVFSTKAYDRQFLEAANAPKHHELAFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVTVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYRNPEMESLGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS
>Mature_341_residues
MKVAVFSTKAYDRQFLEAANAPKHHELAFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVTVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYRNPEMESLGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=265, Percent_Identity=32.8301886792453, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4557497, Length=265, Percent_Identity=32.8301886792453, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI23308577, Length=280, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI145580578, Length=272, Percent_Identity=32.3529411764706, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4557499, Length=272, Percent_Identity=32.3529411764706, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI145580575, Length=269, Percent_Identity=32.7137546468402, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=27.9863481228669, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=57.3170731707317, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082289, Length=306, Percent_Identity=28.4313725490196, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=31.4655172413793, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788660, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=265, Percent_Identity=29.4339622641509, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=262, Percent_Identity=29.3893129770992, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=202, Percent_Identity=38.6138613861386, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=31.5126050420168, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=30.672268907563, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=27.8301886792453, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=26.4285714285714, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=122, Percent_Identity=34.4262295081967, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24646446, Length=281, Percent_Identity=35.2313167259786, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24646448, Length=281, Percent_Identity=35.2313167259786, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24646452, Length=281, Percent_Identity=35.2313167259786, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24646450, Length=281, Percent_Identity=35.2313167259786, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI62472511, Length=281, Percent_Identity=35.2313167259786, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921140, Length=252, Percent_Identity=31.7460317460317, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=26.984126984127, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571528, Length=230, Percent_Identity=29.5652173913043, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45552429, Length=269, Percent_Identity=26.0223048327138, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=26.0223048327138, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=26.0223048327138, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574284, Length=269, Percent_Identity=26.0223048327138, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=217, Percent_Identity=28.110599078341, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585516, Length=224, Percent_Identity=21.875, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37459; Mature: 37459

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVFSTKAYDRQFLEAANAPKHHELAFFEPRLNQDTAILAAGFPAVCVFVHDQVDAAT
CEEEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEEEEECCCCHHH
LEILASRGTRLIVLRCAGFNNVDLKAANKLGVTVVRVPAYSPYGVAEHAVGLILSLNRKI
HHHHHCCCCEEEEEEECCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILGQIMKGFGCRLLAYDVYRNPE
HHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEHHCCCC
MESLGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
HHHHCCCEEEHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEH
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFT
HHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHH
EDALRNIAETTLNNIADIEQGRSCPNEIRYQPEVEAKVLVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MKVAVFSTKAYDRQFLEAANAPKHHELAFFEPRLNQDTAILAAGFPAVCVFVHDQVDAAT
CEEEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEEEEECCCCHHH
LEILASRGTRLIVLRCAGFNNVDLKAANKLGVTVVRVPAYSPYGVAEHAVGLILSLNRKI
HHHHHCCCCEEEEEEECCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILGQIMKGFGCRLLAYDVYRNPE
HHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEHHCCCC
MESLGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
HHHHCCCEEEHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEH
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFT
HHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHH
EDALRNIAETTLNNIADIEQGRSCPNEIRYQPEVEAKVLVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]