Click here to switch to the map view.

The map label for this gene is hemL [H]

Identifier: 161353744

GI number: 161353744

Start: 3936567

End: 3937865

Strand: Direct

Name: hemL [H]

Synonym: alr3265

Alternate gene names: 161353744

Gene position: 3936567-3937865 (Clockwise)

Preceding gene: 17230752

Following gene: 17230758

Centisome position: 61.38

GC content: 47.34

Gene sequence:

>1299_bases
TTGGTAAATACCACTATTAAAACCACAAAATCACAAGAAATCTTCGCCGCCGCCCAAAACCTGATGCCAGGTGGTGTTAG
TTCCCCTGTGCGCGCTTTTAAATCTGTAGGGGGGCAACCCATTGTATTTGATCATGTAAAGGGTGCATACATCTGGGATG
TAGATGGCAACCAATACATTGACTATGTAGGCACTTGGGGGCCTGCTATTTGCGGTCATGCTCATCCAGATGTCATCGGG
GCGTTGCATGATGCTTTAGAAAAAGGTACTAGTTTCGGTGCGCCTTCATTCTTGGAAAATGTTTTGGCGGAAATGGTCAT
CGCTGCTGTTCCCAGCATCGAAATGGTAAGATTCGTCAACTCAGGGACAGAAGCTTGTATGGCTGTATTACGGCTTATGC
GGGCTTTCACTAACCGGGAGAAAGTCATCAAGTTTGAAGGCTGTTACCACGGACACGCCGATATGTTCCTGGTCAAAGCC
GGCTCTGGTGTAGCCACACTAGGTTTACCCGATTCCCCAGGTGTGCCAAAATCTGCAACCAGCAGCACTCTCACAGCCCC
ATACAATGACCTCGAAGCTGTGAAGGCTTTATTTGAGGAAAACCGCGATCAAATCGCTGGTGTCATTCTCGAACCAGTTG
TTGGGAATGCTGGGTTTATTACTCCTGATGCCGGCTTTTTGGAAGGACTGCGGGAACTAACTCACGAACATGGAGCTTTA
CTCGTGTTTGATGAAGTGATGACCGGTTTCCGTATTGCTTATGGTGGCGCTCAAGAAAAGTTTGGCGTTACACCCGATTT
GACGACTTTAGGCAAAGTTATCGGTGGTGGTTTGCCAGTAGGAGCCTATGGTGGTCGTCGGGATATTATGTCCATGATTG
CGCCTGCGGGGCCTGTATATCAAGCAGGAACGCTTTCTGGTAATCCTTTAGCAATGACAGCCGGAATTAAAACTCTGGAG
TTATTACAAAAGCCCGGTGCTTATGAGTATCTAGAGCGAATTACTAAGAAGTTAGCCGATGGGTTACTACAAGTTGCCCT
GGAAACAGGTCATGCAGCTTGCGGTGGTCATATTAGCGCCATGTTTGGCTTATTCTTCACCTCTGGCCCTGTGCATAACT
ATGAAGATGCCAAAAATTCTGACACAGCTAAATTTGGACGCTTCCATCGCGGTATGTTGGAGAGGGGTGTTTACTTAGCA
CCTTCCCAATTTGAGGCTGGTTTTACCTCTTTAGCTCACACTGACGAAGATATTGACCAGACAATTGCGATCGCCCGCGA
AGTTCTATCTAGTATCTAA

Upstream 100 bases:

>100_bases
TTTTTTGGTTCTGCTTATTTTATACGATAGAAGTTCTAGGCGATTTTTAACCCTATCCTGAGATAGAGCTTTTTTTGTTG
CCCATTGTTAGGAGAGACCC

Downstream 100 bases:

>100_bases
ATACTAAGTGCGGAGGGCTGAGTTATTATACTCAGCCCTCCAAAATCAGAACTTTTTAACAGCCACAGCCATTATGGTTA
CAGCCTTTTATAGTTTTGTG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVNTTIKTTKSQEIFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYIDYVGTWGPAICGHAHPDVIG
ALHDALEKGTSFGAPSFLENVLAEMVIAAVPSIEMVRFVNSGTEACMAVLRLMRAFTNREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSATSSTLTAPYNDLEAVKALFEENRDQIAGVILEPVVGNAGFITPDAGFLEGLRELTHEHGAL
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMIAPAGPVYQAGTLSGNPLAMTAGIKTLE
LLQKPGAYEYLERITKKLADGLLQVALETGHAACGGHISAMFGLFFTSGPVHNYEDAKNSDTAKFGRFHRGMLERGVYLA
PSQFEAGFTSLAHTDEDIDQTIAIAREVLSSI

Sequences:

>Translated_432_residues
MVNTTIKTTKSQEIFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYIDYVGTWGPAICGHAHPDVIG
ALHDALEKGTSFGAPSFLENVLAEMVIAAVPSIEMVRFVNSGTEACMAVLRLMRAFTNREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSATSSTLTAPYNDLEAVKALFEENRDQIAGVILEPVVGNAGFITPDAGFLEGLRELTHEHGAL
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMIAPAGPVYQAGTLSGNPLAMTAGIKTLE
LLQKPGAYEYLERITKKLADGLLQVALETGHAACGGHISAMFGLFFTSGPVHNYEDAKNSDTAKFGRFHRGMLERGVYLA
PSQFEAGFTSLAHTDEDIDQTIAIAREVLSSI
>Mature_432_residues
MVNTTIKTTKSQEIFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYIDYVGTWGPAICGHAHPDVIG
ALHDALEKGTSFGAPSFLENVLAEMVIAAVPSIEMVRFVNSGTEACMAVLRLMRAFTNREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSATSSTLTAPYNDLEAVKALFEENRDQIAGVILEPVVGNAGFITPDAGFLEGLRELTHEHGAL
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMIAPAGPVYQAGTLSGNPLAMTAGIKTLE
LLQKPGAYEYLERITKKLADGLLQVALETGHAACGGHISAMFGLFFTSGPVHNYEDAKNSDTAKFGRFHRGMLERGVYLA
PSQFEAGFTSLAHTDEDIDQTIAIAREVLSSI

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=317, Percent_Identity=31.5457413249211, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI13994255, Length=332, Percent_Identity=25.9036144578313, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI37574042, Length=310, Percent_Identity=27.4193548387097, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI24119277, Length=317, Percent_Identity=26.1829652996845, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI284507298, Length=162, Percent_Identity=37.037037037037, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI226442705, Length=311, Percent_Identity=27.0096463022508, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226442709, Length=280, Percent_Identity=27.5, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=55.7647058823529, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1789016, Length=352, Percent_Identity=31.25, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788044, Length=288, Percent_Identity=35.4166666666667, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789759, Length=312, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI145693181, Length=309, Percent_Identity=31.3915857605178, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787560, Length=310, Percent_Identity=30.6451612903226, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786991, Length=309, Percent_Identity=25.8899676375405, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=29.3333333333333, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71992977, Length=357, Percent_Identity=27.1708683473389, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144274, Length=147, Percent_Identity=35.3741496598639, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=302, Percent_Identity=29.4701986754967, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=341, Percent_Identity=22.8739002932551, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357415, Length=332, Percent_Identity=28.6144578313253, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356575, Length=320, Percent_Identity=28.125, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45857; Mature: 45857

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTTIKTTKSQEIFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYI
CCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEE
DYVGTWGPAICGHAHPDVIGALHDALEKGTSFGAPSFLENVLAEMVIAAVPSIEMVRFVN
EECCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
SGTEACMAVLRLMRAFTNREKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPKSAT
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
SSTLTAPYNDLEAVKALFEENRDQIAGVILEPVVGNAGFITPDAGFLEGLRELTHEHGAL
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMIAPAGPVY
EEEHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCE
QAGTLSGNPLAMTAGIKTLELLQKPGAYEYLERITKKLADGLLQVALETGHAACGGHISA
ECCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MFGLFFTSGPVHNYEDAKNSDTAKFGRFHRGMLERGVYLAPSQFEAGFTSLAHTDEDIDQ
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHHH
TIAIAREVLSSI
HHHHHHHHHHCC
>Mature Secondary Structure
MVNTTIKTTKSQEIFAAAQNLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYI
CCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEE
DYVGTWGPAICGHAHPDVIGALHDALEKGTSFGAPSFLENVLAEMVIAAVPSIEMVRFVN
EECCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
SGTEACMAVLRLMRAFTNREKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPKSAT
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
SSTLTAPYNDLEAVKALFEENRDQIAGVILEPVVGNAGFITPDAGFLEGLRELTHEHGAL
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
LVFDEVMTGFRIAYGGAQEKFGVTPDLTTLGKVIGGGLPVGAYGGRRDIMSMIAPAGPVY
EEEHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCE
QAGTLSGNPLAMTAGIKTLELLQKPGAYEYLERITKKLADGLLQVALETGHAACGGHISA
ECCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MFGLFFTSGPVHNYEDAKNSDTAKFGRFHRGMLERGVYLAPSQFEAGFTSLAHTDEDIDQ
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHHH
TIAIAREVLSSI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA