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The map label for this gene is hisH [H]

Identifier: 308065959

GI number: 308065959

Start: 2154061

End: 2154651

Strand: Direct

Name: hisH [H]

Synonym: NA

Alternate gene names: 308065959

Gene position: 2154061-2154651 (Clockwise)

Preceding gene: 16765404

Following gene: 16765406

Centisome position: 44.35

GC content: 57.02

Gene sequence:

>591_bases
ATGAACGTCGTCATCCTTGACACCGGCTGCGCCAATTTAAGCTCGGTAAAATCCGCCGTGGCGCGCCACGGTTACACCCC
GGTGGTCAGCCGCGAAGCGGAAATCGTATTACGCGCTGACAAACTCTTTCTGCCCGGCGTCGGCACGGCGCAGGCCGCTA
TGGATCAACTGCGCGAACGTGAACTGATCGACTTAATTAAAGCGTGTACTCAGCCGGTACTGGGTATCTGCTTAGGGATG
CAACTGCTGGGTCGTCGCAGCGAAGAGACGCGCGGCGTGGATCTGCTGAACATTATCGAACAAGACGTGCCGAAAATGAC
CGACTTTGGTCTGCCGCTGCCGCACATGGGCTGGAATCGCGTGTATCCGCAGGCGGGCAACCGGCTGTTTCAGGGCATTG
AAGATGGCGCCTATTTTTACTTTGTTCACAGCTACGCGATGCCGGTCAACCCGTGGACTATCGCCCAGTGCAATTACGGC
GAGCCGTTTACCGCGGCGGTACAGAAAGATAATTTCTTCGGCGTACAGTTCCACCCGGAACGTTCGGGCGCGGCGGGCGC
ACAGTTGCTGAAAAACTTCCTGGAGATGTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:
NA

Sequences:

>Translated_196_residues
MNVVILDTGCANLSSVKSAVARHGYTPVVSREAEIVLRADKLFLPGVGTAQAAMDQLRERELIDLIKACTQPVLGICLGM
QLLGRRSEETRGVDLLNIIEQDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM
>Mature_196_residues
MNVVILDTGCANLSSVKSAVARHGYTPVVSREAEIVLRADKLFLPGVGTAQAAMDQLRERELIDLIKACTQPVLGICLGM
QLLGRRSEETRGVDLLNIIEQDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=90.8163265306122, Blast_Score=380, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=35.4066985645933, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21704; Mature: 21704

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVILDTGCANLSSVKSAVARHGYTPVVSREAEIVLRADKLFLPGVGTAQAAMDQLRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHH
ELIDLIKACTQPVLGICLGMQLLGRRSEETRGVDLLNIIEQDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFFGVQFHPE
CCHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCEEEEECCCEEEEEECCC
RSGAAGAQLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVVILDTGCANLSSVKSAVARHGYTPVVSREAEIVLRADKLFLPGVGTAQAAMDQLRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHH
ELIDLIKACTQPVLGICLGMQLLGRRSEETRGVDLLNIIEQDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFFGVQFHPE
CCHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCEEEEECCCEEEEEECCC
RSGAAGAQLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]