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The map label for this gene is fumB [H]

Identifier: 16767550

GI number: 16767550

Start: 4545365

End: 4547011

Strand: Reverse

Name: fumB [H]

Synonym: STM4300

Alternate gene names: 16767550

Gene position: 4547011-4545365 (Counterclockwise)

Preceding gene: 16767551

Following gene: 16767547

Centisome position: 93.61

GC content: 54.89

Gene sequence:

>1647_bases
ATGTCGAAAAAAGAGTTTATCTATCAGGCGCCTTTCCCGATGGGAGAGGACAAAACAGAGTATTATCTGCTCACCTCCGA
TTATGTCAGCGTCAGTGAATTCAATGGAGAATCTATCCTCAACGTTGAGCCGCAGGCGTTAACGTTGCTGGCTCAACAGG
CCTTCCATGATGCGTCTTTTATGCTCCGTCCTGAACACCAGCAGCAAGTCGCCGCCATTCTCCATGACCCCGAAGCCAGC
GAAAACGACAAATATGTGGCGTTGCAATTCCTGAGAAACTCCGAGATCGCCGCCAAAGGCATTCTGCCGACCTGCCAGGA
TACCGGCACGGCGATTATCATGGGTAAAAAAGGCCAACGCGTCTGGACGGGCGGCGGTGATGAAGCCGCGCTCTCCAAAG
GCGTGTTTAATACCTATATCGAAGATAACCTGCGCTACTCGCAGAACGCGCCGCTGAATATGTACAAAGAGGTCAATACC
GGCAGCAACCTGCCAGCGCAAATCGATCTCTATGCCGTCGATGGCGACGAGTACAAGTTCCTTTGCGTGGCGAAAGGCGG
CGGTTCAGCAAACAAAACGTATCTCTATCAGGAGACCAAAGCGCTGCTCACCCCAGGCAAGCTGAAAAACTTCCTCGTTG
AGAAAATGCGCACGCTGGGCACCGCGGCCTGTCCGCCATACCATATCGCCTTTGTCATTGGCGGCACCTCGGCGGAAAGC
ACGCTGAAAACCGTTAAGCTCGCCAGCACCCACTATTACGACGCGCTGCCGGCGGAAGGTAACGAGCACGGCCAGGCATT
CCGCGATCCCCACCTGGAACAGGAACTGTTGGAAGAAGCGCAAAAACTTGGCCTCGGCGCCCAGTTCGGCGGGAAATATT
TCGCCCACGATATTCGCGTAATTCGCCTGCCGCGTCACGGCGCTTCCTGTCCGGTGGGGATGGGCGTCTCCTGCTCGGCC
GACCGTAACATCAAAGCGAAAATCAACCGCGAAGGCATCTGGATCGAAAAACTGGAACACAATCCGGGCCAGTATATTCC
GCCAGCGCTGCGTCAGGCGGGCGAAGGCGACGCGGTGAAAGTCGATCTCAACCGTCCGATGAAAGAGATCCTCGCCCAGC
TATCGCAGTACCCGGTCTCGACTCGTCTGTCGCTGACGGGCACCATTATCGTAGGTCGCGATATCGCCCACGCGAAGCTA
AAAGAGCGTATTGAATCCGGCGAAGACCTGCCGCAGTATATTAAAGACCATCCCATCTATTACGCAGGTCCGGCAAAAAC
CCCGGCAGGTTATCCATCTGGCTCATTAGGCCCCACCACCGCAGGCCGAATGGACTCTTATGTCGATCTGCTGCAATCCC
ACGGCGGCAGTATGATCATGCTGGCGAAAGGCAACCGTAGTCAGCAGGTCACGGATGCCTGTAAAAAACATGGCGGCTTT
TATTTGGGCAGTATCGGCGGCCCGGCGGCGGTACTGGCACAGCAGAGCATTAAGCATCTGGAGTGCGTCGAGTATCCGGA
ACTCGGCATGGAAGCTATCTGGAAAATTGAAGTAGAAGATTTCCCGGCCTTTATCCTGGTGGATGACAAAGGCAACGATT
TCTTCCAGCAGATTGTCAGCAAACAGTGCGCTAACTGCGCGAAATAA

Upstream 100 bases:

>100_bases
TGTAATGGCCGCGCGCGCCGCCCGGTTATGCGCGGCGCGCGACTTAGAGCCTGGCCCATGAGGTTCTTAATACATTTTCT
TACTATTAGGCTGGAAGCAC

Downstream 100 bases:

>100_bases
CCTGGCAGGCCCGGCGTTCTCCCGGGCCGCGTTATCGGTTAATTGCCGGATGGCGCTACGCTTATCCGGCTTATCCGGCC
TGCACGAGAGACCATGCTCA

Product: fumarase B

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSKKEFIYQAPFPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASFMLRPEHQQQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALSKGVFNTYIEDNLRYSQNAPLNMYKEVNT
GSNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAES
TLKTVKLASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPPALRQAGEGDAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KERIESGEDLPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQQSIKHLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVSKQCANCAK

Sequences:

>Translated_548_residues
MSKKEFIYQAPFPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASFMLRPEHQQQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALSKGVFNTYIEDNLRYSQNAPLNMYKEVNT
GSNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAES
TLKTVKLASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPPALRQAGEGDAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KERIESGEDLPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQQSIKHLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVSKQCANCAK
>Mature_547_residues
SKKEFIYQAPFPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASFMLRPEHQQQVAAILHDPEASE
NDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALSKGVFNTYIEDNLRYSQNAPLNMYKEVNTG
SNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAEST
LKTVKLASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLEHNPGQYIPPALRQAGEGDAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLK
ERIESGEDLPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACKKHGGFY
LGSIGGPAAVLAQQSIKHLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVSKQCANCAK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=92.8832116788321, Blast_Score=1067, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=88.1386861313869, Blast_Score=1011, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.8342541436464, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60049; Mature: 59918

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKEFIYQAPFPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASF
CCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHCCCCE
MLRPEHQQQVAAILHDPEASENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEEEECCCCCE
VWTGGGDEAALSKGVFNTYIEDNLRYSQNAPLNMYKEVNTGSNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAES
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRV
HHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPPALRQAGEGDAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKERIESGEDLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVS
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCAK
HHHHCCCC
>Mature Secondary Structure 
SKKEFIYQAPFPMGEDKTEYYLLTSDYVSVSEFNGESILNVEPQALTLLAQQAFHDASF
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHCCCCE
MLRPEHQQQVAAILHDPEASENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIMGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEEEECCCCCE
VWTGGGDEAALSKGVFNTYIEDNLRYSQNAPLNMYKEVNTGSNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAES
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTHYYDALPAEGNEHGQAFRDPHLEQELLEEAQKLGLGAQFGGKYFAHDIRV
HHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPPALRQAGEGDAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKERIESGEDLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVS
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCAK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]