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The map label for this gene is melB

Identifier: 16767549

GI number: 16767549

Start: 4543837

End: 4545267

Strand: Direct

Name: melB

Synonym: STM4299

Alternate gene names: 16767549

Gene position: 4543837-4545267 (Clockwise)

Preceding gene: 16767548

Following gene: 39546396

Centisome position: 93.54

GC content: 52.48

Gene sequence:

>1431_bases
ATGAGCATCTCTCTGACAACAAAGCTGAGTTACGGGTTCGGTGCGTTTGGTAAGGATTTCGCCATCGGCATTGTGTATAT
GTACCTGATGTATTACTACACCGATGTGGTGGGACTTTCGGTCGGCCTCGTCGGCACCCTCTTTCTGGTCGCGCGAATCT
GGGATGCGATAAACGATCCCATCATGGGCTGGATTGTCAACGCCACGCGTTCGCGGTGGGGGAAATTTAAGCCGTGGATA
TTGATCGGCACCTTAACCAATTCGCTGGTGCTTTTCCTGCTGTTCAGCGCCCATCTTTTTGAGGGAACCGCGCAGGTTGT
ATTTGTCTGCGTAACCTACATCCTGTGGGGCATGACGTATACCATTATGGATATCCCATTTTGGTCGCTGGTACCGACTA
TTACGCTTGATAAGCGAGAACGCGAACAACTGGTGCCGTTCCCGCGTTTCTTCGCCAGTCTGGCTGGCTTCGTCACTGCC
GGTATAACGCTGCCGTTTGTGAGCTATGTTGGCGGGGCGGATCGTGGGTTCGGCTTTCAGATGTTTACGCTGGTACTGAT
TGCGTTCTTTATCGCCTCGACTATCGTGACATTACGCAACGTACATGAGGTGTACTCCTCCGACAACGGTGTAACGGCAG
GCCGCCCACATCTGACGTTAAAAACGATCGTTGGATTGATATACAAAAACGATCAGCTCTCTTGCCTGTTGGGAATGGCG
CTGGCGTATAACATTGCCTCCAATATTATCAATGGCTTTGCGATCTACTACTTCACCTATGTGATTGGCGATGCCGATCT
TTTTCCCTATTACCTTTCTTACGCCGGCGCGGCGAATCTGCTGACGCTGATTGTCTTCCCCCGGCTGGTGAAAATGTTAT
CGCGGCGGATATTGTGGGCGGGCGCCTCCGTGATGCCCGTTCTGAGTTGCGCAGGGCTCTTCGCGATGGCGTTGGCGGAT
ATCCATAATGCCGCTTTAATCGTGGCGGCGGGTATTTTCCTGAATATCGGGACCGCGCTCTTTTGGGTGCTTCAGGTGAT
CATGGTGGCGGATACGGTCGATTATGGGGAATTTAAGCTCAATATTCGCTGCGAGAGTATCGCTTATTCCGTACAGACGA
TGGTTGTGAAGGGCGGCTCGGCGTTTGCGGCGTTCTTTATCGCTCTGGTGCTGGGGCTGATTGGCTACACGCCGAACGTG
GCGCAGTCTGCGCAAACCCTGCAGGGGATGCAGTTTATTATGATTGTCCTGCCGGTACTGTTTTTCATGATGACGTTGGT
TCTCTACTTCCGCTACTACCGTTTGAACGGCGATATGCTGCGCAAGATTCAGATCCACCTGCTGGATAAATACCGGAAAA
CGCCGCCATTCGTCGAACAGCCGGATAGCCCGGCGATTTCTGTGGTAGCGACCAGCGATGTAAAAGCGTGA

Upstream 100 bases:

>100_bases
CCTGGTTACGCCGTTAATCTCCAGCCGCGCTGGACGGCAGATCCGGCGTTTAACCTCTACTAACAGAAGGCGGGCGCTGG
TAACAGCGACCCGGTACCCT

Downstream 100 bases:

>100_bases
GCATGGTCTCTCGTGCAGGCCGGATAAGCCGGATAAGCGTAGCGCCATCCGGCAATTAACCGATAACGCGGCCCGGGAGA
ACGCCGGGCCTGCCAGGTTA

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTA
GITLPFVSYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
LAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALAD
IHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNV
AQSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA

Sequences:

>Translated_476_residues
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTA
GITLPFVSYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
LAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALAD
IHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNV
AQSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
>Mature_475_residues
SISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWIL
IGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAG
ITLPFVSYVGGADRGFGFQMFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMAL
AYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWAGASVMPVLSCAGLFAMALADI
HNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKLNIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVA
QSAQTLQGMQFIMIVLPVLFFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA

Specific function: Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system)

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family

Homologues:

Organism=Escherichia coli, GI1790561, Length=472, Percent_Identity=84.7457627118644, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=462, Percent_Identity=29.2207792207792, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786466, Length=459, Percent_Identity=27.4509803921569, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI48994989, Length=476, Percent_Identity=25.6302521008403, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI145693206, Length=465, Percent_Identity=25.5913978494624, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787902, Length=478, Percent_Identity=24.4769874476987, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MELB_SALTY (P30878)

Other databases:

- EMBL:   X62101
- EMBL:   AE006468
- PIR:   S23576
- RefSeq:   NP_463164.1
- ProteinModelPortal:   P30878
- GeneID:   1255825
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM4299
- NMPDR:   fig|99287.1.peg.4137
- HOGENOM:   HBG467489
- OMA:   RYPMSIS
- ProtClustDB:   PRK10429
- BioCyc:   STYP99287:STM4299-MONOMER
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043
- TIGRFAMs:   TIGR00792

Pfam domain/function: SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 52801; Mature: 52670

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x421947f8)-; HASH(0x1471a918)-; HASH(0x147123d8)-; HASH(0x421a9d44)-; HASH(0x1477da54)-; HASH(0x145638ec)-; HASH(0x146dec28)-; HASH(0x14718e48)-; HASH(0x14793b94)-; HASH(0x147663e0)-; HASH(0x421b584c)-; HASH(0x421c7570)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IMGWIVNATRSRWGKFKPWILIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTY
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAGITLPFVSYVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASVMPVLSCAGLFAMALADIHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVAQSAQTLQGMQFIMIVLPVL
EEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCH
>Mature Secondary Structure 
SISLTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IMGWIVNATRSRWGKFKPWILIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYILWGMTY
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TIMDIPFWSLVPTITLDKREREQLVPFPRFFASLAGFVTAGITLPFVSYVGGADRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MFTLVLIAFFIASTIVTLRNVHEVYSSDNGVTAGRPHLTLKTIVGLIYKNDQLSCLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLLTLIVFPRLVKMLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GASVMPVLSCAGLFAMALADIHNAALIVAAGIFLNIGTALFWVLQVIMVADTVDYGEFKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NIRCESIAYSVQTMVVKGGSAFAAFFIALVLGLIGYTPNVAQSAQTLQGMQFIMIVLPVL
EEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FFMMTLVLYFRYYRLNGDMLRKIQIHLLDKYRKTPPFVEQPDSPAISVVATSDVKA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1495487; 11677609