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The map label for this gene is melA [H]

Identifier: 16767548

GI number: 16767548

Start: 4542398

End: 4543753

Strand: Direct

Name: melA [H]

Synonym: STM4298

Alternate gene names: 16767548

Gene position: 4542398-4543753 (Clockwise)

Preceding gene: 16767540

Following gene: 16767549

Centisome position: 93.51

GC content: 56.05

Gene sequence:

>1356_bases
ATGATGACGGCACCCAAAATTACCTTTATCGGCGCAGGTTCTACGATTTTCGTCAAAAATATCCTCGGCGATGTGTTTCA
CCGCGAGGCGCTAAAGTCAGCGCATGTCGCCCTGATGGATATTGACGAAACCCGGCTGGAAGAGTCGCACATTGTGGTAC
GGAAACTGATGGACTCAGCGGGCGCTTCTGGCCGGATTACCTGCCATACCAACCAGAAAGCGGCGCTACAGGATGCGGAT
TTCGTGGTGGTCGCCTTTCAGATTGGCGGCTATGAACCCTGCACCGTGACCGATTTTGAGGTTTGTAAGCGTCATGGCCT
GGAACAGACGATCGCCGATACGCTGGGGCCGGGCGGCATCATGCGCGCGCTGCGGACCATCCCGCATCTGTGGCGGATTT
GCGAAGACATGACGGAAGTCTGTCCGAAGGCCACCATGCTCAATTACGTCAACCCGATGGCGATGAATACCTGGGCGATG
TATGCCCGTTATCCGCATATCAAACAGGTCGGCCTGTGCCATTCGGTACAGGGAACGGCGGAAGAACTGGCGCGCGACCT
GAATATCGATCCCACCTCGCTGCGCTACCGCTGCGCCGGCATTAACCACATGGCGTTTTACCTCGAACTGGAGCGCAAAA
CGGCTGACGGGACTTATGTCAATCTCTATCCTGAATTGCTGGCGGCCTATGACGCCGGACAGGCGCCGAAGCCCAATATT
CACGGCAATGAACGCTGCCAGAACATCGTGCGCTATGAGATGTTCAAAAAGTTGGGCTACTTCGTCACTGAGTCATCAGA
GCATTTTGCCGAGTACACGCCGTGGTTTATTAAACCGGGACGCGAGGATCTGATTGCGCGCTACAAGGTGCCGCTGGATG
AATATCCGAAACGCTGCGTAGAACAACTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAACCGCCGAGCGTATCGAC
ATCAAACCGTCCCGCGAGTACGCCAGCACCATTATGAACGCTCTGTGGACCGGCGAGCCGAGCGTGATTTACGGCAATGT
GCGTAACGAGGGGCTGATTGATAACCTGCCGCAGGGAAGCTGCGTGGAAGTGGCTTGTCTGGTGGATGCCAACGGCATTC
AACCGACGAAGGTGGGGACGATCCCTTCCCATCTGGCGGCGATGATGCAGACCAACATCAACGTGCAAACGCTGTTGACC
GAAGCCATCCTCACGGAAAACCGCGATCGCGTGTATCACGCGGCGATGATGGACCCTCATACCGCAGCGGTGCTGGGTAT
CGAAGAAATCTATGCGTTGGTTGACGATCTGATCGCCGCGCATGGCGACTGGCTTCCGGCCTGGTTACGCCGTTAA

Upstream 100 bases:

>100_bases
CATACGATCTGAGTTTTCGGGAATGCTCATCGGGGAAGACGGAGGTTTTCACCGGGAACGCGTGCCACCATGAAGGTGTT
CACTACTGTTCTGGAGAGTC

Downstream 100 bases:

>100_bases
TCTCCAGCCGCGCTGGACGGCAGATCCGGCGTTTAACCTCTACTAACAGAAGGCGGGCGCTGGTAACAGCGACCCGGTAC
CCTATGAGCATCTCTCTGAC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTNQKAALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWRICEDMTEVCPKATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPTSLRYRCAGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNI
HGNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERID
IKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR

Sequences:

>Translated_451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTNQKAALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWRICEDMTEVCPKATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPTSLRYRCAGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNI
HGNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERID
IKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR
>Mature_451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSAGASGRITCHTNQKAALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWRICEDMTEVCPKATMLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPTSLRYRCAGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNI
HGNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCVEQLANWHKELEEYKTAERID
IKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGSCVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIEEIYALVDDLIAAHGDWLPAWLRR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=94.2350332594235, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=24.7826086956522, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50736; Mature: 50736

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHC
GASGRITCHTNQKAALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWRICEDMTEVCPKATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPTSLRYRCAGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNI
HHHHHHCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCC
HGNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKTAERIDIKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
CVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIEEIYALVDDLIAAHGDWLPAWLRR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MMTAPKITFIGAGSTIFVKNILGDVFHREALKSAHVALMDIDETRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHC
GASGRITCHTNQKAALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWRICEDMTEVCPKATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPTSLRYRCAGINHMAFYLELERKTADGTYVNLYPELLAAYDAGQAPKPNI
HHHHHHCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCEEEECHHHHHHHCCCCCCCCCC
HGNERCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIARYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKTAERIDIKPSREYASTIMNALWTGEPSVIYGNVRNEGLIDNLPQGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
CVEVACLVDANGIQPTKVGTIPSHLAAMMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIEEIYALVDDLIAAHGDWLPAWLRR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]