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The map label for this gene is thiH

Identifier: 16767413

GI number: 16767413

Start: 4377022

End: 4378155

Strand: Reverse

Name: thiH

Synonym: STM4159

Alternate gene names: 16767413

Gene position: 4378155-4377022 (Counterclockwise)

Preceding gene: 16767414

Following gene: 16767410

Centisome position: 90.13

GC content: 54.41

Gene sequence:

>1134_bases
ATGAAAACCTTCACCGACCGTTGGCGGCAACTGGAGTGGGACGATATTCGCCTGCGCATCAATGGTAAAACCGCCGCTGA
CGTGGAACGTGCGCTGAACGCCGCGCATCTTAGCCGGGATGATTTGATGGCACTGCTCTCCCCCGCCGCCGCCGATTATC
TGGAACCGATAGCGCAGCGGGCGCAAAGGCTGACCCGACAACGCTTTGGCAACACCGTCAGTTTCTACGTGCCGCTTTAT
CTCTCAAACCTCTGTGCCAACGACTGTACCTACTGCGGTTTTTCGATGAGCAACCGCATTAAGCGTAAAACGCTGGATGA
GGTGGATATTCAAAGGGAATGCGATGCTATCCGTAAACTGGGCTTTGAGCATCTGCTGTTAGTCACCGGCGAACATCAGG
CCAAAGTCGGCATGGACTATTTTCGCCGTCATTTACCCACCATCCGCCGTCAATTTTCCTCTTTACAGATGGAAGTCCAG
CCCTTGTCGCAAGAAAACTATGCGGAGCTCAAAACACTGGGGATCGATGGCGTGATGGTTTATCAGGAGACTTATCATGA
GGCAATCTATGCACAGCATCACCTGAAGGGAAAGAAACAGGACTTTTTCTGGCGGCTGGAAACGCCGGATCGGTTAGGCC
GGGCAGGTATCGACAAAATCGGTCTTGGCGCGCTAATTGGTCTGTCGGACAACTGGCGGGTGGATTGCTATATGGTGGCG
GAGCATCTGTTGTGGATGCAGAAACACTACTGGCAGAGTCGCTATTCTGTTTCCTTCCCGCGTCTGCGTCCGTGTACTGG
CGGTGTGGAACCCGCATCTGTGATGGATGAAAAGCAACTGGTGCAAACGATTTGCGCTTTCCGGTTATTGGCGCCGGAAA
TTGAATTATCACTCTCCACCCGCGAATCGCCGTGGTTTCGAGACCATGTGATTCCTCTGGCAATCAACAACGTCAGCGCC
TTCTCAAAAACGCAGCCCGGCGGCTACGCTGACGATCATCCGGAACTGGAGCAGTTTTCTCCCCACGATGCCCGTCGGCC
TGAAACAGTAGCAAGCGCGTTAAGCGCGCAAGGATTGCAGCCCGTCTGGAAAGACTGGGACAGTTGGCTGGGGCGCGCTT
CGCAAACGCGGTGA

Upstream 100 bases:

>100_bases
CTGTAGAAGCGGGAGTACTGGCGCGTCAGGCGGTGCCGGGTAATCGTAGCACCTACGCCAACGCCACCAGCCCGCTCACC
GGATTTCTGGAGGCGTTAGC

Downstream 100 bases:

>100_bases
ACCCGTATGTAAACGCATTAGTATTTCACGAACGAGTACGTAATGCGAAAAACGGGAGATTGTTATCGACCTCCCGTTTT
TTTATTTTGTCATCGTCAGC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQPVWKDWDSWLGRASQTR

Sequences:

>Translated_377_residues
MKTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQPVWKDWDSWLGRASQTR
>Mature_377_residues
MKTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQPVWKDWDSWLGRASQTR

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family

Homologues:

Organism=Escherichia coli, GI1790423, Length=375, Percent_Identity=89.3333333333333, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIH_SALTY (Q9S498)

Other databases:

- EMBL:   AF154064
- EMBL:   AF170176
- EMBL:   AE006468
- RefSeq:   NP_463028.1
- ProteinModelPortal:   Q9S498
- GeneID:   1255685
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM4159
- NMPDR:   fig|99287.1.peg.4001
- HOGENOM:   HBG296822
- OMA:   NLCANEC
- ProtClustDB:   PRK09240
- BioCyc:   STYP99287:STM4159-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- TIGRFAMs:   TIGR02351

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 43448; Mature: 43448

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQR
CCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQPLSQENYAELKTLGIDGVMV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCEEEE
YQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHH
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDHVIPLAINNVSAFSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQTR
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQR
CCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQPLSQENYAELKTLGIDGVMV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCEEEE
YQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHH
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDHVIPLAINNVSAFSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQTR
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609