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The map label for this gene is priA [H]

Identifier: 16767361

GI number: 16767361

Start: 4303361

End: 4305559

Strand: Reverse

Name: priA [H]

Synonym: STM4095

Alternate gene names: 16767361

Gene position: 4305559-4303361 (Counterclockwise)

Preceding gene: 16767363

Following gene: 16767360

Centisome position: 88.64

GC content: 59.16

Gene sequence:

>2199_bases
ATGTCCGTCGCGCACGTTGCCTTGCCCGTTCCGCTTCCCCGAACCTTTGATTACCTGCTGCCGGAAGGCGGGGTCGCCAA
AGCCGGGTGCCGCGTGCGCGTGCCATTCGGCAAGCAGCAAGAGCGCGTGGGCATTGTCGTCTCGATTAGCGACCACAGCG
AATTGCCGCTCGATGAATTAAAATCGGTCATTGAAATCCTGGATAACGAGCCAATCTTTTCTCCCTCCATCTGGCGCTTA
CTCCTCTGGGCGGCGGATTACTACCACCATCCCCTCGGCGACGTTCTGTTTCACGCTCTGCCGGTTTTATTGCGTCAGGG
AAAACCAGCCAGCAACGCGCCCCTATGGTATTGGTTCGCCACCGAAGAGGGGCAAGCCGTCGATATCAACAGCCTGAAAC
GCTCCGCCAAACAACAACAGGCGCTGGCGGCGCTACGGCAAGGAAAGATCTGGCGCTATCAGGTAGCCGAGCTTGATTTT
ACTGACGCCACGCTGCAAACGCTGCGGCGTAAGGGACTTTGTGAGCTTGCCAGTGAAACGCCCGCGTTTACCGACTGGCG
GGAGCGTTATGCCGTCGCAGGAGAGCGCCTGCGGCTGAATACCGAACAGGCGACAGCAGTCGGCGCGATTCACAGCGCGT
CGGACGGTTTCTCCGCCTGGTTGCTGGCAGGCGTAACCGGTTCCGGCAAGACCGAAGTGTATCTGAGCGTGCTGGAAAAC
GTCCTCGCCCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACGATCGCCCGTTTTCGCGA
ACGTTTTAACGCGCCGGTGGAAGTGCTGCATTCCGGGCTAAACGACAGCGAGCGTCTTTCGGCATGGCTGAAAGCGAAAA
ACGGCGAAGCGGCGATTGTCATCGGCACGCGTTCCTCGCTGTTTACGCCGTTTAAAAATCTTGGCGTGATTGTCATCGAC
GAAGAGCACGACAGCTCTTATAAACAGCAGGAAGGGTGGCGCTACCACGCCCGCGATTTGGCAGTGTATCGCGCCCATAG
CGAGCAGATCCCGATTATTCTCGGCTCCGCCACCCCAGCCCTGGAAACGCTATGTAACGTGCGGCAGAAAAAATACCGGA
TGCTGCGTCTGACGCGGCGCGCAGGCAACGCCCGCCCAGCTCTGCAGCATGTGCTGGATTTAAAAGGCCAGCGTCTTCAG
GCGGGACTGGCGCCCGCCCTTATCGCCCGTATGCGCCAACATCTCCAGGCCGACAATCAGGTAATTTTATTTCTTAACCG
CCGGGGATTCGCGCCCGCGCTGCTTTGCCACGACTGCGGCTGGATTGCAGAGTGCCCGCGTTGCGATCACTACTACACGC
TGCACCAGGCGCAGCATCATTTACGCTGTCATCACTGCGACAGCCAGCGTCCGGTACCGCGTCAGTGCCCCTCCTGCGGC
TCCACACATATGTTACCGGTTGGGTTAGGGACGGAACAGCTGGAGCAGGTGCTGGCGCCCTTCTTCCCCGGCGTGCCGAT
CTCACGAATCGACCGTGATACCACCAGTCGTAAAGGCGCGCTGGAGCAGCATCTGGCGGAAGTTCACCGCGGCGGAGCAC
GCATCCTGATAGGCACACAAATGCTGGCGAAAGGCCATCACTTTCCGGATGTCACGCTGGTGGCGTTGCTGGATGTCGAT
GGCGCGCTTTTTTCCGCAGATTTTCGTTCGGCGGAACGCTTCGCCCAGCTCTACACTCAGGTTTCCGGCCGCGCCGGGCG
CGCGGGCAAACAGGGCGAAGTGGTGCTCCAAACGCACCATCCTGAGCATCCCCTGCTGCAAACGTTACTGTATAAAGGCT
ATGATGCGTTTGCGGAGCAGGCGCTGGCGGAACGGCAAACGCTCCAGCTTCCGCCGTGGACCAGCCACGTCATTATTCGC
GCAGAGGATCATAACAATCAGCAGGCGCCCGTCTTTCTCCAACAGTTGCGTAATCTGATTCAGGCCAGCCCGCTATCAGA
CGATAAGCTGTGGATCTTAGGTCCGGTTCCGGCCCTCGCGCCAAAACGCGGCGGCCGCTATCGCTGGCAAATTTTACTTC
AGCATCCTTCACGGATTCGCCTGCAACACATCATCAGCGGCACGCTGGCGTTGATCAATACGCTCCCGGAAGCGCGGAAA
GTTAAATGGGTTCTGGACGTCGATCCGATTGAAGGATAG

Upstream 100 bases:

>100_bases
GGCGAATCATACAGAATTTGACCACCGTATGCAAACTGATCCGCGCTCCCTTATCGTAATGTGTATACTACCCGACGAAA
TTTCAAGTCAGGAAGATGCT

Downstream 100 bases:

>100_bases
TCCTCACGATGCGAGGCGGATCGTAAAATTAAAGATCCATCACACTTTTTATGAAAATTCTGTAACCGCTTCCACGCACT
ATCTGATAAAAATGTTTTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSISDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWRERYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
VKWVLDVDPIEG

Sequences:

>Translated_732_residues
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSISDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWRERYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
VKWVLDVDPIEG
>Mature_731_residues
SVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSISDHSELPLDELKSVIEILDNEPIFSPSIWRLL
LWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFT
DATLQTLRRKGLCELASETPAFTDWRERYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLENV
LAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVIDE
EHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQA
GLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGS
THMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRA
EDHNNQQAPVFLQQLRNLIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARKV
KWVLDVDPIEG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=90.5737704918033, Blast_Score=1395, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81773; Mature: 81642

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSISDHSELPLDEL
CCCEEEEECCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWRERYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHEEECCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC
>Mature Secondary Structure 
SVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSISDHSELPLDEL
CCEEEEECCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWRERYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHEEECCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRIRLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]