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The map label for this gene is rbsR [H]

Identifier: 16767170

GI number: 16767170

Start: 4096544

End: 4097542

Strand: Direct

Name: rbsR [H]

Synonym: STM3886

Alternate gene names: 16767170

Gene position: 4096544-4097542 (Clockwise)

Preceding gene: 16767169

Following gene: 16767174

Centisome position: 84.34

GC content: 53.35

Gene sequence:

>999_bases
TTGGCTACTATGAAAGATGTTGCCCGCCTGGCGGGAGTCTCTACATCGACGGTCTCTCACGTTATTAACAAAGATCGCTT
TGTGAGTGAGACGATAACCGAAAAAGTTGAAGCGGCGATTAAATCGCTCAACTATGCGCCTTCTGCGCTGGCGCGAAGCC
TCAAGTTAAACCAGACCCGCACGATTGGTATGCTAATTACCGCCAGTACCAACCCTTTTTATTCCGAACTGGTGCGCGGC
GTTGAGCGAAGCTGTTTTGAGCGCGGTTATAGTCTGGTGCTGTGCAATACCGAAGGCGACGAGCAACGGATGAACCGCAA
CCTGGAGACGCTGATGCAAAAACGGGTAGACGGTTTACTGCTGCTGTGTACCGAAACGCATCAGCCTTCTCGTGAAATCA
TGGAGCGTTATCCGTCTATTCCTACCGTCATGATGGACTGGTCGCCGTTTGATGGTGAGGGCGATAGCGATCTCATTCAG
GATAATTCGTTGCTAGGCGGCGATCTTGCTACCCAATACCTTATCGATAAAGGATTTACCCGAATAGCCTGTATTACCGG
CCCGCTGGACAAAACGCCAGCGCGTTTACGGCTGGAAGGGTATCGGGCGGCGATGAAACGCGCAGGGCTGGCTATTCCGC
AAGGCTATGAGATCACCGGCGATTTTGAATTTGGCGGCGGCTTTGACGCCATGCAGGCGTTACTCGCCCATCAACAGCGA
CCGCAAGCGGTTTTTACCGGAAACGATGCGATGGCTGTCGGCGCCTATCAGGCGCTATATCAGGCGGGGCTTCGTATTCC
GCAGGATATGGCAGTCATTGGCTATGATGATATTGAGCTGGCGCGCTATATGACGCCGCCGCTGACGACTATCCATCAGC
CGAAAGATGAATTGGGCGAGCTGGCTATTGATGTGTTGATTCATCGGATGGCGCAGCCCGCCTTGCAACAACAGCGACTT
CAGCTTACTCCGGTTTTGACGGTGCGTGGGTCGGTTTAG

Upstream 100 bases:

>100_bases
CCATGCCGCCGCCGCGATTGCCGTAACGCGTAAAGGCGCGCAACCTTCCGTTCCGTGGCGCGAAGAGATAGAAGCGTTTT
TACATCAGCAGGGGTAACGC

Downstream 100 bases:

>100_bases
ATTTATGCCGTTCTTTAATCAGATTGTTGCCGTCTTTGGCTCTTAGCAGCATAAACATCAGCGCGGACACGATAGTGATC
GCCCCCATCGTGATAAAGGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQ
DNSLLGGDLATQYLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRL
QLTPVLTVRGSV

Sequences:

>Translated_332_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQ
DNSLLGGDLATQYLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRL
QLTPVLTVRGSV
>Mature_331_residues
ATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQD
NSLLGGDLATQYLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQRP
QAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRLQ
LTPVLTVRGSV

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=90.0302114803625, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=46.5671641791045, Blast_Score=317, Evalue=5e-88,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.5987055016181, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=33.6734693877551, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=29.8192771084337, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=32.1321321321321, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=29.5819935691318, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=27.8125, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994940, Length=334, Percent_Identity=28.7425149700599, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786268, Length=320, Percent_Identity=26.25, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=25.0764525993884, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.4019292604502, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789456, Length=342, Percent_Identity=26.6081871345029, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790192, Length=250, Percent_Identity=28.4, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=25.1572327044025, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36720; Mature: 36588

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTR
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQDNSLLGGDLATQYLIDKGFT
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCE
RIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
EEEEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGE
CCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHH
LAIDVLIHRMAQPALQQQRLQLTPVLTVRGSV
HHHHHHHHHHHHHHHHHHHHEECEEEEECCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQDNSLLGGDLATQYLIDKGFT
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCE
RIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
EEEEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGE
CCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHH
LAIDVLIHRMAQPALQQQRLQLTPVLTVRGSV
HHHHHHHHHHHHHHHHHHHHEECEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]