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The map label for this gene is trmE [H]

Identifier: 16767128

GI number: 16767128

Start: 4048213

End: 4049577

Strand: Direct

Name: trmE [H]

Synonym: STM3843

Alternate gene names: 16767128

Gene position: 4048213-4049577 (Clockwise)

Preceding gene: 16767127

Following gene: 16767129

Centisome position: 83.34

GC content: 59.63

Gene sequence:

>1365_bases
ATGAGCCATAACGACACTATCGTCGCCCAGGCAACCCCACCGGGACGCGGTGGCGTGGGTATCCTGCGTATTTCCGGTCT
GAAGGCGCGCGACGTTGCGCAAGAGGTGCTGGGCAAGCTGCCGAAGCCGCGCTATGCCGACTACCTGCCGTTTAAAGATG
TTGACGGCAGCGCGCTGGATCAGGGTATTGCGCTGTGGTTCCCCGGCCCGAACTCCTTCACCGGTGAAGATGTGCTGGAG
CTACAAGGGCACGGCGGTCCGGTGATTCTGGACCTGCTGTTAAAACGTATCCTGACGCTTCCAGGCGTGCGCATTGCCCG
ACCGGGCGAGTTCTCCGAGCGAGCGTTCCTCAACGATAAGCTCGATTTAGCCCAGGCGGAAGCCATTGCCGACCTGATCG
ATGCCAGTTCCGAACAGGCGGCGCGTTCGGCGCTAAACTCGTTACAGGGGGCATTTTCCGCCCGCGTTAACCACCTTGTG
GAAGCACTTACTCACCTGCGAATCTACGTCGAAGCGGCTATTGATTTTCCGGATGAGGAGATCGACTTTCTCTCTGACGG
CAAAATCGAAGCGCAGCTCAATGGCGTCATCGCCGATCTTGACGCCGTGCGCACCGAAGCGCGCCAGGGCAGCCTGCTGC
GCGAAGGGATGAAGGTAGTGATTGCCGGACGCCCGAATGCCGGGAAATCCAGCCTGCTGAATGCGCTGGCAGGCCGTGAA
GCGGCCATTGTCACCGATATCGCCGGTACCACGCGCGACGTGTTGCGCGAGCATATCCACATCGACGGGATGCCGCTGCA
TATCATCGATACCGCCGGACTGCGCGACGCCAGCGACGAAGTTGAGCGTATCGGCATCGAACGCGCCTGGCAGGAGATTG
AACAGGCCGATCGCGTGCTGTTTATGGTGGATGGCACCACGACGGACGCCGTTGACCCTGCCGACATCTGGCCGGACTTT
ATCGCCCGTCTGCCCAAAAATCTACCGATTACCGTGGTACGTAACAAGGCGGATATCACCGGCGAAACGTTGGGTATTAG
TGAAGTAAACGGTCACTCACTGGTTCGCCTCTCGGCGCGTACCGGCGAAGGCATCGACGTGTTGCGTAACCACCTCAAGC
AAAGCATGGGGTTTGATACCAATATGGAAGGCGGCTTCCTGGCCCGTCGCCGTCACCTACAGGCGCTGGCGGAAGCCGCT
GAACACCTTGAGCAGGGCAAAGCGCAGCTATTAGGCGCATGGGCGGGGGAACTGCTGGCGGAAGAGTTGCGCCTGGCGCA
GCAAAGCTTAAGCGAGATTACCGGCGAGTTTACCTCCGACGACCTGCTGGGACGGATTTTCTCCAGCTTCTGTATCGGTA
AGTAA

Upstream 100 bases:

>100_bases
GCGCGCCGGTCAGCCTGATGGCGCTAACGCTTATCAGGCCTACAATCAAAAGGCGGTCATATGACCGCCTTTTTTTATTG
CAACAAAGTTGAGACTAACC

Downstream 100 bases:

>100_bases
GTTTTAGTCCACCCTCGTCAGTGAACGTTCACTAACCCGCATTAACTGCCTGTTAACGCGGGTTTTTACTTTCCATACTC
GTCCATAGTCGTGCGGGTTC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDF
IARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAA
EHLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDF
IARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAA
EHLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDFI
ARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAAE
HLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=36.1111111111111, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=33.8, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=35.7142857142857, Blast_Score=228, Evalue=8e-60,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=94.7136563876652, Blast_Score=868, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=40.6593406593407, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=457, Percent_Identity=28.4463894967177, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=31.9018404907975, Blast_Score=212, Evalue=9e-56,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=32.5051759834368, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49106; Mature: 48975

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALD
CCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPADIWPDFIARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSAR
EEECCCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAAEHLEQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALD
CCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPADIWPDFIARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSAR
EEECCCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAAEHLEQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA