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The map label for this gene is spoU [H]

Identifier: 16767028

GI number: 16767028

Start: 3936186

End: 3936875

Strand: Direct

Name: spoU [H]

Synonym: STM3743

Alternate gene names: 16767028

Gene position: 3936186-3936875 (Clockwise)

Preceding gene: 16767027

Following gene: 16767029

Centisome position: 81.03

GC content: 54.49

Gene sequence:

>690_bases
ATGAATCCAAAACGTTATGCGCGTATATGCGAAATGCTCGCCAGGCGTCAGCCTGATCTCACGGTCTGCATGGAGCAAGT
CCACAAACCTCATAACGTTTCTGCAATCATTCGTACCGCAGATGCCGTTGGCGTCCATGAAGTCCATGCCATCTGGCCAG
GTAGCCGGATGCGCACCATGGCTTCTGCGGCGGCGGGCAGCAACAGTTGGGTACAGGTGAAAACTCACCGTACCATTGGC
GATGCGGTAGCCCATTTGAAAAGTCGGGGAATGCAGATTCTGGCAACCCATCTTTCTGATAAGGCCGTAGACTTTCGCGA
GATTGATTACACGCGCCCGACCTGTATTCTGATGGGTCAGGAGAAAACCGGTATTACTCAGGAAGCATTAGCCCTGGCGG
ATCGGGATATCATCATTCCGATGATCGGCATGGTACAGTCGCTGAACGTTTCCGTCGCCTCCGCGCTCATTCTTTATGAA
GCCCAGCGCCAGCGACAGAACGCGGGCATGTATCTGCGTGAAAATAGCATGTTGCCGGAAGATGAACAGCAGCGCCTGCT
GTTTGAGGGTGGTTATCCGGTGCTGGCGAAAGTCGCTAAACGTAAAGGTCTGCCTTATCCTCGCGTCAATCAGCAGGGCG
AAATCGACGCCGATGCTGACTGGTGGGCGACGATGCAGGCGGCAGGGTAA

Upstream 100 bases:

>100_bases
CGCCTTACCGCACGCGATCGCGTACATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTCATTAAAGTCAC
CCGTAACCGAAACTAGCGCT

Downstream 100 bases:

>100_bases
TCACCATGTCAGGCCGCTTGTTAGACGCTGTTCCGCTCAATTCCCTGACAGGCGTCGGCGCAGCGCAAAGCAGTAAACTG
GCGAAAATTGGCCTGCACAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKSRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG

Sequences:

>Translated_229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKSRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
>Mature_229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKSRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=229, Percent_Identity=96.0698689956332, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1790623, Length=160, Percent_Identity=32.5, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25534; Mature: 25534

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTM
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKSRGMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCHHEEHHHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
CHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEECCC
>Mature Secondary Structure
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTM
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKSRGMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCHHHHHHHHHHHCCHHEEHHHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
CHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]