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The map label for this gene is treF [H]

Identifier: 16766889

GI number: 16766889

Start: 3775574

End: 3777223

Strand: Direct

Name: treF [H]

Synonym: STM3603

Alternate gene names: 16766889

Gene position: 3775574-3777223 (Clockwise)

Preceding gene: 16766883

Following gene: 16766891

Centisome position: 77.73

GC content: 55.21

Gene sequence:

>1650_bases
ATGCTCAACCAGAAATTAAACCCCACGCCTTCTGAAGATCTGACTATTGATGTCGATCTGCTTTATGAAACAGACCCGTG
CGAGTTAAAGCTGGATGAAATGATCGAGGCGGAACCGGAGCCGGAGATGATCGAGGGGCTTCCCGCCTCTGACGCCCTGA
CCCCTGCCGATCGGTATCTCGAACTGTTCGAGCATGTACAGTCAACAAAACTGTTTCCAGACAGTAAAACGTTCCCGGAC
TGCGCGCCGAAGATGGACCCGCTTGATATCCTGATCCGCTACCGTAAGGTCAGACGCCACCGTGATTTTGACTTACGCCG
CTTCGTCGAGAACCATTTCTGGCTACCGGAAACCTTATCCAGCGAGTATGTCTCTAATCCCGAAAACTCGCTCAAAGAAC
ATATTGACCAGCTTTGGCCGATACTGACGCGCGAACCGCAGGATCATATTCCGTGGTCTTCGCTGCTGGCGCTGCCGCAA
TCCTATATCGTGCCGGGAGGCCGTTTTAGCGAAACCTATTATTGGGACTCTTATTTTACCATGCTGGGTCTGGCGGAAAG
CGGGCGCGAAGACTTACTTAAATGTATGGCGGATAATTTCGCCTGGATGATTGAAAACTATGGCCATATTCCCAACGGCA
ACCGGACCTATTATCTGAGCCGTTCGCAGCCGCCGGTTTTTGCCCTGATGGTTGAACTGTTCGAAGAAGATGGCGTGCGC
GGCGCGCGGCGTTATCTCGACCATCTCAAAATGGAATACGCCTTCTGGATGGACGGCGCCGAATCGCTGGCGCTCAACCA
GGCTTATCGCCACGTTGTGCGGATGCCGGACGGTTCCCTGCTGAACCGCTACTGGGACGATCGCGACACGCCGCGCGACG
AGTCGTGGCTGGAAGATGTCGAAACTGCAAAACACTCCGGTCGTCCGCCCAATGAAGTCTACCGGGATTTGCGCGCCGGG
GCGGCGTCCGGCTGGGACTATTCTTCACGCTGGCTACGCGATGCGGGCCGGCTTGCCAGCATACGTACGACCCAGTTTAT
CCCTATCGATTTGAACGCTTTTCTGTATAAACTGGAGAGCGCTATCGCCAATATTTCAGCACTGAAAGGCGAACGTGACA
CCGAGGCGCTATTTCGCCAGAAAGCCAGCGATCGACGCGCCGCCGTGAACCATTATCTGTGGGATGATGAAAACGGCTGT
TATCGGGACTATGACTGGCGACGCGAAGAAATGGCGCTCTTTTCCGCCGCCAGTATCGTGCCGCTGTATGTCGGTATGGC
GAACCACGAGCAGGCCGACCGTCTGGCAAATGTGGTACGCAGCCGTCTGTTAACGCCCGGCGGGATTATGGCGACCGAAT
ACGAAACCGGCGAACAGTGGGATAAACCGAACGGCTGGGCACCGCTGCAATGGATGGCGATTCAGGGCTTCAAACGGTAT
GGCGACGATATGCTGGGCGATGAAATCGCCCATAACTGGTTAAAAACAGTGAACCATTTTTATCAGGAGCACCATAAACT
GATCGAGAAATACCATATTTCCGGCGGTACGCCCCGCGAAGGCGGCGGGGGCGAGTATCCGCTACAGGACGGGTTCGGCT
GGACCAATGGCGTGGTGCGCCGCCTGATTGGCCTTTACGGAGAGCCTTAA

Upstream 100 bases:

>100_bases
CACACTTTGCCACTCAGGTATCGAAGCGAATGTCTACGCTTAATAGACAGCCGGGCAACACCCGTATCTTTTACGACGCA
AGCCGGAGGTCCTTGTCATT

Downstream 100 bases:

>100_bases
CCTGCTCCGGCCTCAGTGAATCGCATCATAAATGGCTGACTGAATTTTCAGCCATTTTTTATTGTCTGCGCTGGGCCGGG
CCTGAGCGCGCAATCGGGCC

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSTKLFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLSSEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLESAIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGC
YRDYDWRREEMALFSAASIVPLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY
GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Sequences:

>Translated_549_residues
MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSTKLFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLSSEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLESAIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGC
YRDYDWRREEMALFSAASIVPLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY
GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP
>Mature_549_residues
MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSTKLFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLSSEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLESAIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGC
YRDYDWRREEMALFSAASIVPLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY
GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=529, Percent_Identity=35.7277882797732, Blast_Score=260, Evalue=3e-69,
Organism=Escherichia coli, GI1789936, Length=550, Percent_Identity=92, Blast_Score=1046, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=50.7186858316222, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.8785578747628, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17565078, Length=510, Percent_Identity=31.9607843137255, Blast_Score=239, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI25141398, Length=530, Percent_Identity=31.5094339622642, Blast_Score=231, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI25148109, Length=531, Percent_Identity=30.6967984934087, Blast_Score=231, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71987755, Length=416, Percent_Identity=31.25, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=473, Percent_Identity=28.5412262156448, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319473, Length=485, Percent_Identity=29.2783505154639, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24656680, Length=548, Percent_Identity=31.5693430656934, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24656675, Length=548, Percent_Identity=31.5693430656934, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24656661, Length=533, Percent_Identity=31.7073170731707, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17933716, Length=533, Percent_Identity=31.7073170731707, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24656670, Length=533, Percent_Identity=31.7073170731707, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24656685, Length=484, Percent_Identity=32.2314049586777, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI22024178, Length=538, Percent_Identity=26.2081784386617, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45551104, Length=380, Percent_Identity=26.0526315789474, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28573474, Length=295, Percent_Identity=26.7796610169492, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63645; Mature: 63645

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCCEEEEEEEECCCCCCEEHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSTKLFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLS
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
SEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCEEEEECHHHHHHHHHH
AIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGCYRDYDWRREEMALFSAASIV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHH
PLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC
>Mature Secondary Structure
MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCCEEEEEEEECCCCCCEEHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSTKLFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLS
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
SEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCEEEEECHHHHHHHHHH
AIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGCYRDYDWRREEMALFSAASIV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHH
PLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA