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The map label for this gene is nirC

Identifier: 16766764

GI number: 16766764

Start: 3626647

End: 3627456

Strand: Direct

Name: nirC

Synonym: STM3476

Alternate gene names: 16766764

Gene position: 3626647-3627456 (Clockwise)

Preceding gene: 16766763

Following gene: 16766765

Centisome position: 74.66

GC content: 56.05

Gene sequence:

>810_bases
ATGTTTACAGACACTATCAATAAGTGTGCGGCTAACGCTGCGCGCATCGCACGCCTGTCGGCGAATAATCCGCTCGGCTT
CTGGGTCAGTTCGGCAATGGCCGGGGCTTATGTCGGTCTCGGCATCATTCTTATTTTCACCCTCGGCAATCTGCTCGACC
CGTCCGTACGTCCTCTGGTGATGGGAGCCACCTTCGGTATCGCCTTAACGCTGGTCATCATCGCCGGTTCCGAACTGTTT
ACCGGCCACACCATGTTCCTGACGCTGGGCGTCAAAGCAGGCACCATCAGCCACGGTCAAATGTGGGCTATCCTGCCGCA
AACCTGGCTCGGCAACCTGGTCGGTTCCGTGTTTGTCGCCCTGCTTTACAGCTGGGGCGGCGGCAGTTTATTGCCGGTCG
ATACCAGCATCGTTCACTCAGTCGCGCTGGCGAAAACCACCGCGCCCGCCACGGTACTGTTCTTCAAAGGCGCGCTGTGT
AACTGGCTGGTTTGTCTGGCAATCTGGATGGCAATCCGCACCGAAGGCACGGCAAAATTTCTTGCTATCTGGTGGTGTCT
GCTGGCGTTTATCGCTTCCGGCTACGAGCACTCCGTCGCGAATATGACGCTGTTCGCCCTCTCCTGGTTTGGTCATCACA
GCGACGCCTATACCCTGGCCGGAATTGGTCATAACCTGTTGTGGGTGACACTCGGTAATACTTTGTCCGGTGTCGTATTC
ATGGGATTGGGTTATTGGTATGCTACGCCAAAATCGGAGCGTCCGGCTCCGGCAAAAATCAATCAGCCAGAGGCTGCTGC
CAATAATTAA

Upstream 100 bases:

>100_bases
TCATTAATCCCCAGGAGCATAGTTCACTATGTGACTGGGGTGAGCGAGGGCAGCCAACAAAGAGGCAGTGCAAAGGATGA
AGTGAGAAAAGGATAATCAA

Downstream 100 bases:

>100_bases
GGGGTAATGTCGTGGACCATTTGCCTATATTTTGTCAATTACGCGACCGCGACTGTCTGATCGTCGGCGGTGGCGATGTC
GCAGAACGCAAAGCACGGTT

Product: nitrite transporter NirC

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALC
NWLVCLAIWMAIRTEGTAKFLAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYTLAGIGHNLLWVTLGNTLSGVVF
MGLGYWYATPKSERPAPAKINQPEAAANN

Sequences:

>Translated_269_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALC
NWLVCLAIWMAIRTEGTAKFLAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYTLAGIGHNLLWVTLGNTLSGVVF
MGLGYWYATPKSERPAPAKINQPEAAANN
>Mature_269_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALC
NWLVCLAIWMAIRTEGTAKFLAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYTLAGIGHNLLWVTLGNTLSGVVF
MGLGYWYATPKSERPAPAKINQPEAAANN

Specific function: May act as a nitrite transporter

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family

Homologues:

Organism=Escherichia coli, GI87082249, Length=267, Percent_Identity=92.8838951310861, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1787132, Length=260, Percent_Identity=28.4615384615385, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321779, Length=211, Percent_Identity=27.4881516587678, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIRC_SALTY (P25926)

Other databases:

- EMBL:   M64606
- EMBL:   AE006468
- PIR:   A39200
- RefSeq:   NP_462379.1
- GeneID:   1254999
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3476
- NMPDR:   fig|99287.1.peg.3356
- HOGENOM:   HBG726755
- OMA:   ITAGFEH
- ProtClustDB:   PRK11562
- BioCyc:   STYP99287:STM3476-MONOMER
- InterPro:   IPR000292
- TIGRFAMs:   TIGR00790

Pfam domain/function: PF01226 Form_Nir_trans

EC number: NA

Molecular weight: Translated: 28636; Mature: 28636

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01005 FORMATE_NITRITE_TP_1; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11c36a64)-; HASH(0x1142ee44)-; HASH(0x11811094)-; HASH(0x11adc46c)-; HASH(0x123a2858)-; HASH(0x11ff754c)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLV
CCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MGATFGIALTLVIIAGSELFTGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVA
EHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHH
LLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALCNWLVCLAIWMAIRTEGTAKF
HHHHCCCCCEEECCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYTLAGIGHNLLWVTLGNTLSGVVF
HHHHHHHHHHHHCCCCHHHHHHHHEEHHHHCCCCCCEEEECCCCCEEEEEECCHHHHHHH
MGLGYWYATPKSERPAPAKINQPEAAANN
HHHHHEEECCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLV
CCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MGATFGIALTLVIIAGSELFTGHTMFLTLGVKAGTISHGQMWAILPQTWLGNLVGSVFVA
EHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHH
LLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALCNWLVCLAIWMAIRTEGTAKF
HHHHCCCCCEEECCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYTLAGIGHNLLWVTLGNTLSGVVF
HHHHHHHHHHHHCCCCHHHHHHHHEEHHHHCCCCCCEEEECCCCCEEEEEECCHHHHHHH
MGLGYWYATPKSERPAPAKINQPEAAANN
HHHHHEEECCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1987123; 11677609