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The map label for this gene is fruK [H]

Identifier: 16766552

GI number: 16766552

Start: 3423470

End: 3424384

Strand: Direct

Name: fruK [H]

Synonym: STM3254

Alternate gene names: 16766552

Gene position: 3423470-3424384 (Clockwise)

Preceding gene: 16766551

Following gene: 16766553

Centisome position: 70.48

GC content: 43.28

Gene sequence:

>915_bases
GTGATCTACACGCTAACACTTAATAGCGCGATTGATATGAATATATTTAGCGATCCTTTACAGCCAAATATTGTCAATCG
CACTCACCACACCGAGTTTTGCCCAAATGGTAAAGGGGTAAATGTTGCGCTGGTTCTGGATCACTTTCAAATACCTGCGC
ATATATTAGGCATTTTTGGTGGTTTCACCGGGCATTATATTGTAGAAAGCCTGCGTACCCGAAAAATGCCAGTCACACCA
GCATGGGTAGAAGAACCCACGCGGATTAATATTTTCATTCATGACGGTAAGCAGGAATATAAACTGGTCAACCCTGGCTC
ATATATTCCAGATGAATGTAAACAACAAATTATCACGATTATTTCGCAACTGCCCGATGCAGACTATCTGGTGATAAGCG
GCAGCCTGCCACAAGGTATTGAAAGCCGCTTTTACGCAGAAATCATGCATATTTGTCAGCAAAAAAATATCGGCGTAATT
TTAGATATTAGTCATCCGTCATTACGTCAGTTACTGGAGTTCAAACCGTTATTAATTAAACCAAATGATGAAGAGGTTAA
AGCAATATTCGGTTTAACTGTCTCTGACGACAATGATGCGAAAAATACTCTAACCACTTTACATGCACTTGGTGCACAAA
ATGTTCTGCTAACCCTTGGCGCTAAAGGAATGTATTTCTCCAATGGGATTGATTACTGGTTTTGTAGCGCGCCAACGGTT
GATTTGGTGAGCTCCGCCTGTGCAGGCGATGCTGCACTGGCCGCCTTTCTCAGCCAGTGGTTATCTACAGGAGAAGTGGA
ATATGCCTTATCTCTTGCCAGTGCAACAGGAGCTGATGTCGCAAGTTCTGCCGGACTAGGCCAACTTGCGGCAATAGAAA
CCTTACTTTCGCAAATCCACGTCAGAAAGCTGTAG

Upstream 100 bases:

>100_bases
CCCCGCCACTATATGCAACCAGCAAAACAGGCGATGAAAGAGGTGGTACGCAAAGTAATTCATGTTTGCGGCTGTGAAGG
GCAACTGTGAGGAATCGCCT

Downstream 100 bases:

>100_bases
GTTAAATTACAATATAAGGTCTGCTTATGAAAAAGATCATTGCAGTGACCGGCTGCCCTACCGGTATTGCGCATACATTT
ATGGCTGAAGAAGCCTTAAA

Product: fructose-1-phosphate kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIYTLTLNSAIDMNIFSDPLQPNIVNRTHHTEFCPNGKGVNVALVLDHFQIPAHILGIFGGFTGHYIVESLRTRKMPVTP
AWVEEPTRINIFIHDGKQEYKLVNPGSYIPDECKQQIITIISQLPDADYLVISGSLPQGIESRFYAEIMHICQQKNIGVI
LDISHPSLRQLLEFKPLLIKPNDEEVKAIFGLTVSDDNDAKNTLTTLHALGAQNVLLTLGAKGMYFSNGIDYWFCSAPTV
DLVSSACAGDAALAAFLSQWLSTGEVEYALSLASATGADVASSAGLGQLAAIETLLSQIHVRKL

Sequences:

>Translated_304_residues
MIYTLTLNSAIDMNIFSDPLQPNIVNRTHHTEFCPNGKGVNVALVLDHFQIPAHILGIFGGFTGHYIVESLRTRKMPVTP
AWVEEPTRINIFIHDGKQEYKLVNPGSYIPDECKQQIITIISQLPDADYLVISGSLPQGIESRFYAEIMHICQQKNIGVI
LDISHPSLRQLLEFKPLLIKPNDEEVKAIFGLTVSDDNDAKNTLTTLHALGAQNVLLTLGAKGMYFSNGIDYWFCSAPTV
DLVSSACAGDAALAAFLSQWLSTGEVEYALSLASATGADVASSAGLGQLAAIETLLSQIHVRKL
>Mature_304_residues
MIYTLTLNSAIDMNIFSDPLQPNIVNRTHHTEFCPNGKGVNVALVLDHFQIPAHILGIFGGFTGHYIVESLRTRKMPVTP
AWVEEPTRINIFIHDGKQEYKLVNPGSYIPDECKQQIITIISQLPDADYLVISGSLPQGIESRFYAEIMHICQQKNIGVI
LDISHPSLRQLLEFKPLLIKPNDEEVKAIFGLTVSDDNDAKNTLTTLHALGAQNVLLTLGAKGMYFSNGIDYWFCSAPTV
DLVSSACAGDAALAAFLSQWLSTGEVEYALSLASATGADVASSAGLGQLAAIETLLSQIHVRKL

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=284, Percent_Identity=27.112676056338, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788493, Length=303, Percent_Identity=28.0528052805281, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33178; Mature: 33178

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTLTLNSAIDMNIFSDPLQPNIVNRTHHTEFCPNGKGVNVALVLDHFQIPAHILGIFG
CEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHC
GFTGHYIVESLRTRKMPVTPAWVEEPTRINIFIHDGKQEYKLVNPGSYIPDECKQQIITI
CCHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
ISQLPDADYLVISGSLPQGIESRFYAEIMHICQQKNIGVILDISHPSLRQLLEFKPLLIK
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCEEEC
PNDEEVKAIFGLTVSDDNDAKNTLTTLHALGAQNVLLTLGAKGMYFSNGIDYWFCSAPTV
CCCHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCEEEECCCHH
DLVSSACAGDAALAAFLSQWLSTGEVEYALSLASATGADVASSAGLGQLAAIETLLSQIH
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHH
VRKL
HCCC
>Mature Secondary Structure
MIYTLTLNSAIDMNIFSDPLQPNIVNRTHHTEFCPNGKGVNVALVLDHFQIPAHILGIFG
CEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHC
GFTGHYIVESLRTRKMPVTPAWVEEPTRINIFIHDGKQEYKLVNPGSYIPDECKQQIITI
CCHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
ISQLPDADYLVISGSLPQGIESRFYAEIMHICQQKNIGVILDISHPSLRQLLEFKPLLIK
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCEEEC
PNDEEVKAIFGLTVSDDNDAKNTLTTLHALGAQNVLLTLGAKGMYFSNGIDYWFCSAPTV
CCCHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCEEEECCCHH
DLVSSACAGDAALAAFLSQWLSTGEVEYALSLASATGADVASSAGLGQLAAIETLLSQIH
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHH
VRKL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]