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The map label for this gene is metC

Identifier: 16766461

GI number: 16766461

Start: 3320995

End: 3322182

Strand: Direct

Name: metC

Synonym: STM3161

Alternate gene names: 16766461

Gene position: 3320995-3322182 (Clockwise)

Preceding gene: 16766457

Following gene: 16766462

Centisome position: 68.37

GC content: 53.54

Gene sequence:

>1188_bases
ATGACGGATAAACAGTTGGATACCAAACTGGTAAACGCAGGACGCAGCAAAAAATACACGCTTGGCTCAGTGAATAGTGT
GATTCAACGCGCTTCTTCACTGGTGTTTGACACCGTCGAGGCCAAAAAACACGCTACCCGCAATCTCGCCAACGGCGAAT
TATTTTACGGACGCCGGGGAACGCTGACCCACTTTTCGCTCCAGGAAGCCATGTGTGAACTGGAAGGCGGCGCCGGATGC
GCGCTCTTCCCGTGCGGCGCGGCGGCGGTCGCCAACACCATTCTGGCATTCGTTGAGCAGGGCGACCACATCCTGATGAC
CAACACAGCTTATGAACCCAGCCAGGATTTCTGCAGCAAAATTCTCGGCAAACTGGGCGTGACGACAAGCTGGTTCGACC
CGCTCATCGGCGCTGATATCACGCAACATATTCAGCCGAACACGAAAGTCGTATTTCTGGAATCCCCCGGTTCTATTACC
ATGGAAGTACATGATATTCCGAGCATTGTTAGTGCGGTCAGACGCGTCGCGCCAGAAGCCGTCATCATGATTGATAATAC
CTGGGCGGCTGGCGTTCTGTTCAAAGCTCTGGAATTCGATATTGATATCTCTATCCAGGCGGGCACTAAGTATCTGATTG
GTCATTCTGATGCGATGGTCGGGACTGCCGTTGCAAATGCGCGCTGCTGGGAACAATTGCGGGAAAACGCCTATCTGATG
GGGCAAATGCTCGACGCCGACACGGCTTATATGACCAGCCGCGGTCTGCGCACACTCGGTGTCCGACTGCGCCAGCATCA
AGAAAGCAGCCTCAAAATCGCCGCATGGCTGGCGAACCATCCTCAGGTCGCGCGCGTTAATCATCCGGCCCTGCCGGGTA
GCAAAGGTCATGCGTTCTGGAAACGAGACTTTACAGGTAGCAGCGGCCTGTTCTCCTTTGTGCTCAATAAAAAGCTGACC
GAGGCAGAATTATCAGCCTATCTGGATAACTTTTCGCTGTTCAGTATGGCCTACTCCTGGGGAGGCTACGAATCGCTGAT
TATTGCTAATCAGCCGGAACAGATCGCCGCGATTCGTCCCGCAGGCGGCGTAGATTTTACCGGTACGCTGGTTCGGGTGC
ATATTGGTTTAGAGAGTGTTGATGATTTGATCGCTGATTTAGCCGCCGGCTTCGCCAGAATTGTGTAA

Upstream 100 bases:

>100_bases
TTTTGGCAAAATTTTCATCTGTATCACACGTCGCCAGGGTGCAGATGGTTATATTCATGCTAGTTTAGACATCCAGACGG
TTAAAATCAGGAAACGCAAC

Downstream 100 bases:

>100_bases
AGTTGCCGGGGATGGACATATATGCAGACAATTTCCGTGGAAAAGTCTGCGTTTGTTGCGTCCGGGATCAAGGCATCCCG
GACGATTCAGGAGTACAATA

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDKQLDTKLVNAGRSKKYTLGSVNSVIQRASSLVFDTVEAKKHATRNLANGELFYGRRGTLTHFSLQEAMCELEGGAGC
ALFPCGAAAVANTILAFVEQGDHILMTNTAYEPSQDFCSKILGKLGVTTSWFDPLIGADITQHIQPNTKVVFLESPGSIT
MEVHDIPSIVSAVRRVAPEAVIMIDNTWAAGVLFKALEFDIDISIQAGTKYLIGHSDAMVGTAVANARCWEQLRENAYLM
GQMLDADTAYMTSRGLRTLGVRLRQHQESSLKIAAWLANHPQVARVNHPALPGSKGHAFWKRDFTGSSGLFSFVLNKKLT
EAELSAYLDNFSLFSMAYSWGGYESLIIANQPEQIAAIRPAGGVDFTGTLVRVHIGLESVDDLIADLAAGFARIV

Sequences:

>Translated_395_residues
MTDKQLDTKLVNAGRSKKYTLGSVNSVIQRASSLVFDTVEAKKHATRNLANGELFYGRRGTLTHFSLQEAMCELEGGAGC
ALFPCGAAAVANTILAFVEQGDHILMTNTAYEPSQDFCSKILGKLGVTTSWFDPLIGADITQHIQPNTKVVFLESPGSIT
MEVHDIPSIVSAVRRVAPEAVIMIDNTWAAGVLFKALEFDIDISIQAGTKYLIGHSDAMVGTAVANARCWEQLRENAYLM
GQMLDADTAYMTSRGLRTLGVRLRQHQESSLKIAAWLANHPQVARVNHPALPGSKGHAFWKRDFTGSSGLFSFVLNKKLT
EAELSAYLDNFSLFSMAYSWGGYESLIIANQPEQIAAIRPAGGVDFTGTLVRVHIGLESVDDLIADLAAGFARIV
>Mature_394_residues
TDKQLDTKLVNAGRSKKYTLGSVNSVIQRASSLVFDTVEAKKHATRNLANGELFYGRRGTLTHFSLQEAMCELEGGAGCA
LFPCGAAAVANTILAFVEQGDHILMTNTAYEPSQDFCSKILGKLGVTTSWFDPLIGADITQHIQPNTKVVFLESPGSITM
EVHDIPSIVSAVRRVAPEAVIMIDNTWAAGVLFKALEFDIDISIQAGTKYLIGHSDAMVGTAVANARCWEQLRENAYLMG
QMLDADTAYMTSRGLRTLGVRLRQHQESSLKIAAWLANHPQVARVNHPALPGSKGHAFWKRDFTGSSGLFSFVLNKKLTE
AELSAYLDNFSLFSMAYSWGGYESLIIANQPEQIAAIRPAGGVDFTGTLVRVHIGLESVDDLIADLAAGFARIV

Specific function: Methionine biosynthesis; third step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=292, Percent_Identity=31.1643835616438, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI299473759, Length=228, Percent_Identity=31.5789473684211, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI25453487, Length=326, Percent_Identity=27.3006134969325, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=86.5822784810127, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1790375, Length=397, Percent_Identity=31.4861460957179, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560056, Length=329, Percent_Identity=31.9148936170213, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=29.5180722891566, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=28.1914893617021, Blast_Score=158, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=335, Percent_Identity=41.1940298507463, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=354, Percent_Identity=31.3559322033898, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6321254, Length=263, Percent_Identity=27.7566539923954, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=252, Percent_Identity=30.5555555555556, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=25.3554502369668, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922554, Length=337, Percent_Identity=32.3442136498516, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METC_SALTY (P18949)

Other databases:

- EMBL:   AE006468
- PIR:   JV0020
- RefSeq:   NP_462076.1
- ProteinModelPortal:   P18949
- SMR:   P18949
- PRIDE:   P18949
- GeneID:   1254684
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3161
- NMPDR:   fig|99287.1.peg.3052
- HOGENOM:   HBG754729
- OMA:   REQSYLM
- ProtClustDB:   PRK08114
- BioCyc:   STYP99287:STM3161-MONOMER
- BRENDA:   4.4.1.8
- GO:   GO:0005737
- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808:SF8
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434
- TIGRFAMs:   TIGR01324

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.4.1.8

Molecular weight: Translated: 42872; Mature: 42741

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKQLDTKLVNAGRSKKYTLGSVNSVIQRASSLVFDTVEAKKHATRNLANGELFYGRRG
CCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
TLTHFSLQEAMCELEGGAGCALFPCGAAAVANTILAFVEQGDHILMTNTAYEPSQDFCSK
CEEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
ILGKLGVTTSWFDPLIGADITQHIQPNTKVVFLESPGSITMEVHDIPSIVSAVRRVAPEA
HHHHHCCCHHHHHHHHCCCHHHHCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCE
VIMIDNTWAAGVLFKALEFDIDISIQAGTKYLIGHSDAMVGTAVANARCWEQLRENAYLM
EEEEECCHHHHHHHHHHHCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEH
GQMLDADTAYMTSRGLRTLGVRLRQHQESSLKIAAWLANHPQVARVNHPALPGSKGHAFW
HHHHCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCEE
KRDFTGSSGLFSFVLNKKLTEAELSAYLDNFSLFSMAYSWGGYESLIIANQPEQIAAIRP
EECCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCEEEEEEECCCCCEEEECC
AGGVDFTGTLVRVHIGLESVDDLIADLAAGFARIV
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDKQLDTKLVNAGRSKKYTLGSVNSVIQRASSLVFDTVEAKKHATRNLANGELFYGRRG
CCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
TLTHFSLQEAMCELEGGAGCALFPCGAAAVANTILAFVEQGDHILMTNTAYEPSQDFCSK
CEEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
ILGKLGVTTSWFDPLIGADITQHIQPNTKVVFLESPGSITMEVHDIPSIVSAVRRVAPEA
HHHHHCCCHHHHHHHHCCCHHHHCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCE
VIMIDNTWAAGVLFKALEFDIDISIQAGTKYLIGHSDAMVGTAVANARCWEQLRENAYLM
EEEEECCHHHHHHHHHHHCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEH
GQMLDADTAYMTSRGLRTLGVRLRQHQESSLKIAAWLANHPQVARVNHPALPGSKGHAFW
HHHHCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCEE
KRDFTGSSGLFSFVLNKKLTEAELSAYLDNFSLFSMAYSWGGYESLIIANQPEQIAAIRP
EECCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCEEEEEEECCCCCEEEECC
AGGVDFTGTLVRVHIGLESVDDLIADLAAGFARIV
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2659970; 11677609