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The map label for this gene is ascG [H]

Identifier: 16766314

GI number: 16766314

Start: 3171663

End: 3172685

Strand: Direct

Name: ascG [H]

Synonym: STM3012

Alternate gene names: 16766314

Gene position: 3171663-3172685 (Clockwise)

Preceding gene: 16766313

Following gene: 16766316

Centisome position: 65.3

GC content: 57.87

Gene sequence:

>1023_bases
ATGATGGCGACAATGCTGGATGTTTCCCGCCATGCGGGCGTATCAAAGGCCACCGTCTCACGAGTGCTGAATGGGACGGG
GCAGGTAAAAGAAAGCACGCGCCAGAAAGTGTTTACGGCGATGCAGGCTCTGGGCTATCGCCCCAACCTGCTGGCACGCT
CGCTGGCGAATCGCACCAGCAACAGCATCGGTCTGGTCGTCTCTACGTTTGACGGCTTCTATTTCGGCAGTTTGTTGCGC
CGGGCGTCGCGCCAGGCGGAGTCTCATAACAAGCAGTTGATCGTCACCGATGGTCACGATACGCCGGAACGAGAGCAGAA
AGCCGTACAAATGTTGGCCGACAGACAGTGCGACGCTATTATTCTTTACACTCGCTATATGGATGAGCTGGCGATTTTGT
CGTTGATTGACGCCACGGAAATGCCGCTGGTGATTATTAATCGCAACGTCACTCAGGCCCGCGATCGCGCTATTTTCTTC
GAGCAGGAGACGGCGGCATTCCAGGCGGTGGAATATCTGATTACGCAGGGCCATCGCGATATCGCCTGTATTACGCTGCC
TGTTCATACTCCCACCGGCACATCACGCGTAGCGGGTTATCGCAAGGCGCTGGAAAAGTATGGCATTCCCTGGCAACCGG
CAAAAGTGAAATACGGCGATTACACGCTGACGCGCGGCTATGACGCCTGCCGGGAATTACTGGAGGAAGGCGTCACGTTT
AGCGCGCTATTCGCCTGTAATGATGACACGGCGCTGGGCGCGGCAAAAGCGCTGCGCCAGGCCGGATTACGCATCCCACA
GGATGTGTCGCTGTTTGGTTTTGACGATGCGCCGGGCGCAACCTGGCTTGAGCCGGGGCTTTCAACAGTCTATTTACCCA
TCGAGGCTATGATAGCCACCGCGATCGATCAGGCCGTTCGTCTGGCGAACAGCGAGCCGGTCGCCCCGATCCCGCCCTTT
ACCGGCACGCTGATTCTGCGCGAGTCCGTCGCCGCGGGCCCGTTTTTTCAACGTCCGGCCTAA

Upstream 100 bases:

>100_bases
GAAGTAACTCATGTATTTAGTCCGACACTGGTACGCCGACATTCGGTGTCCACGCCGACGGATACCGGGCACCTGTCGAC
AACCGATTAATCAGGGTAGC

Downstream 100 bases:

>100_bases
TGATGCGCTTCAGAGCATCTCCAGCGCTAACAGCTCTTCAATGGTTTGACGACGGCGGATCAGCCGCGCCTGTCCATTGT
CAAACAGCACTTCCGGCAGG

Product: transcriptional regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALGYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAESHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDELAILSLIDATEMPLVIINRNVTQARDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEAMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPA

Sequences:

>Translated_340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALGYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAESHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDELAILSLIDATEMPLVIINRNVTQARDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEAMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPA
>Mature_340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALGYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAESHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDELAILSLIDATEMPLVIINRNVTQARDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEAMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPA

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=41.0179640718563, Blast_Score=273, Evalue=9e-75,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=34.0361445783133, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=34.2342342342342, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=31.547619047619, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790194, Length=302, Percent_Identity=30.794701986755, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790369, Length=292, Percent_Identity=30.8219178082192, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=28.7878787878788, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.875, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=28.6173633440514, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787906, Length=283, Percent_Identity=26.8551236749117, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=22.8668941979522, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786268, Length=156, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=24.0549828178694, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790192, Length=305, Percent_Identity=24.9180327868852, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37306; Mature: 37306

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALGYRPNLLARSLANRTS
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
NSIGLVVSTFDGFYFGSLLRRASRQAESHNKQLIVTDGHDTPEREQKAVQMLADRQCDAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
ILYTRYMDELAILSLIDATEMPLVIINRNVTQARDRAIFFEQETAAFQAVEYLITQGHRD
EEEHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCC
IACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCE
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEAMIAT
EEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHH
AIDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPA
HHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC
>Mature Secondary Structure
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALGYRPNLLARSLANRTS
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
NSIGLVVSTFDGFYFGSLLRRASRQAESHNKQLIVTDGHDTPEREQKAVQMLADRQCDAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
ILYTRYMDELAILSLIDATEMPLVIINRNVTQARDRAIFFEQETAAFQAVEYLITQGHRD
EEEHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCC
IACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCE
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEAMIAT
EEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHH
AIDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPA
HHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]