Click here to switch to the map view.

The map label for this gene is nadB

Identifier: 16765961

GI number: 16765961

Start: 2780428

End: 2782050

Strand: Direct

Name: nadB

Synonym: STM2641

Alternate gene names: 16765961

Gene position: 2780428-2782050 (Clockwise)

Preceding gene: 16765955

Following gene: 16765963

Centisome position: 57.24

GC content: 55.14

Gene sequence:

>1623_bases
ATGATGACAACTCCTGAACTGTCCTGTGATGTGTTAATTATCGGCAGCGGCGCGGCCGGACTCTCGCTGGCGCTGCGTCT
GGCTGAAAAGCATAAGGTTATCGTCCTGAGTAAAGGGCCGGTGAGCGAGGGCTCCACGTTTTATGCGCAGGGCGGTATTG
CCGCCGTGTTTGATGAAACCGACAGTATTGCGTCGCATGTCGAAGATACGCTGATTGCCGGCGCCGGAATTTGCGATCGC
CACGCGGTGGAGTTTGTCGCCAGCAACGCCCGTACCTGCGTGCAGTGGCTTATCGATCAGGGCGTCCTGTTTGATACGCA
TGTCCAGCCAAACGGCAAGGAGAGCTATCATCTCACGCGTGAAGGCGGCCATAGTCATCGCCGTATCCTTCATGCCGCCG
ACGCTACCGGAAAGGAAGTCGAAACCACGTTGGTCAGTCGGGCGCAAAATCATCCTAATATTCAGGTGCTGGAACGCAGC
AACGCCGTTGATTTAATTATCTCCGATAAAATGGGTCTGCCGGGGCCTCGCCGGGTCGTCGGCGCCTGGATCTGGAATCG
CAATAAAGAGTGGGTTGAAACCTGTCATGCGAAGTCGGTGGTGCTGGCGACCGGCGGCGCCTCAAAGGTTTATCAATATA
CCACTAACCCGGATATTTCCTCCGGTGACGGTATCGCGATGGCCTGGCGCGCAGGCTGTCGGGTCGCTAATCTGGAATTT
AACCAGTTTCATCCCACCGCCCTTTATCACCCGCAGGCGCGTAATTTTCTCCTTACCGAAGCGCTGCGTGGCGAAGGCGC
ATACCTTAAACGTCCGGACGGTTCACGGTTTATGCCGGACGTTGACGAACGCGGGGAACTGGCGCCGCGCGATATTGTCG
CCCGCGCTATTGACCATGAGATGAAACAACTCGGCGCAGACTGTATGTTTCTCGATATCAGCCACAAGCCTGACGATTTT
GTCCGCCAGCACTTCCCGATGATCTATGCGAAACTGCTTGATTTGGGGATGGATTTAACCAAAGAACCGATTCCCGTTGT
TCCTGCCGCACACTATACCTGCGGCGGCGTGGTCGTCGATGATTATGGTCGTACCGATGTAGACGGCTTATATGCCATTG
GCGAAGTCAGTTACACCGGCCTGCACGGCGCTAACCGTATGGCGTCCAACTCGTTGCTGGAATGCTTAGTGTACGGCTGG
TCCGCAGCAATGGATATCGACAGGCGAATGCCTTCTGTCCACAGCGTTGACGCCCTTCCCGCCTGGGATGAAAGCCGCGT
GGAAAACGCCGATGAGCGGGTGGTGATTCAGCATAACTGGCATGAACTGCGCCTGCTGATGTGGGACTATGTGGGCATTG
TGCGCACGACGAAACGGCTGGAGCGCGCCCTACGGCGTATTACCATGTTGCAACAAGAGATTGACGAATATTACGCCAAC
TTTCGCGTCTCGAATAATTTACTGGAGCTGCGCAATCTGGTGCAAGTGGCCGAACTGATTGTGCGTTGTGCGATGATGCG
TAAAGAGAGTCGGGGGCTGCACTTTACGCTGGATTATCCGCAACAACTGGCTGAATCAGGCCCATCGATCCTTTCGCCGT
TAACGCCTCACATAAATAGATAA

Upstream 100 bases:

>100_bases
TATGTTTAATAAGCACAACACCAGCGAAATCATAACGTGCTTTTTAGCGCCATATAGTGCTAATCTGCCGCAACCATGTT
TAGTAAATTAAACAAGAACC

Downstream 100 bases:

>100_bases
AATGCCTGGGTCAGCGCCGTATAACTTTCAGAATAATGCTGGTCTGACCCGCGAATTACTAATCTGTCGCTAAAGCACTC
CCCGGCTTGCGGGGAGAATG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKQLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDALPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Sequences:

>Translated_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKQLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDALPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
>Mature_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKMGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKQLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDALPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=31.0810810810811, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1788928, Length=540, Percent_Identity=89.8148148148148, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=529, Percent_Identity=32.5141776937618, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1786942, Length=569, Percent_Identity=30.5799648506151, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=539, Percent_Identity=30.6122448979592, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=537, Percent_Identity=29.608938547486, Blast_Score=185, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI71986328, Length=459, Percent_Identity=27.4509803921569, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=31.1151079136691, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=31.4183123877917, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.734693877551, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.6376811594203, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663005, Length=558, Percent_Identity=30.1075268817204, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI17137288, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655642, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655647, Length=539, Percent_Identity=30.7977736549165, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_SALTY (Q8ZMX9)

Other databases:

- EMBL:   AE006468
- RefSeq:   NP_461576.1
- ProteinModelPortal:   Q8ZMX9
- SMR:   Q8ZMX9
- GeneID:   1254164
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2641
- NMPDR:   fig|99287.1.peg.2555
- HOGENOM:   HBG293998
- OMA:   GAYIWNR
- ProtClustDB:   PRK09077
- BioCyc:   STYP99287:STM2641-MONOMER
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 60110; Mature: 60110

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 244-244 ACT_SITE 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKMGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKQLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDALP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKMGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKQLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPVVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDALP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609