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The map label for this gene is yfgF [H]

Identifier: 16765823

GI number: 16765823

Start: 2618585

End: 2620798

Strand: Reverse

Name: yfgF [H]

Synonym: STM2503

Alternate gene names: 16765823

Gene position: 2620798-2618585 (Counterclockwise)

Preceding gene: 16765825

Following gene: 16765818

Centisome position: 53.95

GC content: 47.7

Gene sequence:

>2214_bases
ATGAAACTAAATAAAACTTATATAAATATCAGAGATAAATGGTGGGGACTACCGCTCATTCTCCCTTCGATTTTACTGCC
TGTTTTAAGTAGCGCAAATACTTATGCGCTTACCAGCACTGGAAATGTGGTTCTTTTTTACCTGCCGTTAGCTTTTATGC
TTAGCCTGATGCTCTTTTTCGGCTGGGCAGCACTGCCGGGCATTGTGCTTGCTATCTTCTGGCGCAGATACCCGCAGACA
GGGCTGTATGAAACCCTTTCCGTGACGATGCACTTTATCATTACTATCGTGCTAAGCTGGGGCGGCTATCGTGTCTTTTC
CCCCCGGCGAAACAATGTTTCCCACGGCGACGCCCACCTGCTGTTTCAACGAATATTCTGGCAGGTATTTTGTTCAGCAA
CATTATTTCTGGTCATCTACCAGTTTGCCGCTTTTGTTGGCATGTACGAAAGCAAAGCCAGCCTGATGGGGGTAATGCCC
TTTAATATCAACACGCTGATTAATTACCAGGCGTTACTGGTCGGTAACCTTGTCGGCGTGCCATTGTGCTATTTTATTAT
TCGTACTCTTCGCAATCCGCTGCACCTACGGGGGTATTATCAGCAATTAAAGCTACAGATTGATAGCAAGGCCACTAAAA
AAGAGATCGTTATTTGGCTGGCCGTATTGACTACACTAATGTTTATACTGTGTATGCCATTGACTGATAATAGTTCAATA
TTTAGTACCAACTATACGCTTTCGTTATTATTGCCGGTCATGCTGTGGGGCGCGATGCGCTATGGCTATAAGTTTATTTC
AATTATCTGGGCCGTCGTGCTAATTACGTCGATTCATTATTATCAGCGTTATATGCCCTGGTATTCAGGTTATGATACGC
AATTAGCGATTACCTCTTCGAGCTATCTGGTTTTTTCTTTTATTGTTAATTATATGTCTGTACTGGCCACGCGTCAGCGC
GTAGTGAGTGGTCGCGCACGGCGGCTGGCCTATCTTGACCCGGTTGTACATCTTCCCAATTTACGTGCGTTAAACCGCGC
GCTCCAAAATGCGCCCTGGTCTACGATCTGTTTTTTACACGTGCCGGGCCTGGAGCTATTGGGTAAAAATTATGGCGTCA
TGCTACGCATCCAGTATAAGCAAAAGCTCTCTCACTGGATTACGCCGATGCTGGCCTCAAATGAGTGCGTCTACCAGATG
TCAGGGCACGACCTGGTGCTGCGTCTTAATACAGAGGCGCATCAGCAGCGTATTGAAGCGCTGGATAAACATATTAAGCA
GTTTCGGTTTATCTGGGATGGACTGCCGCTACAGCCGCCTGTCGGCGTGAGCTATTGCTGCGTGCGTTCTCCGGTTAGCC
ACCTTTATCTGCTGTTGGGCGAATTAAGCACCAGTTCCGATCTTTCGTTGACGACTAACGCGCCGGAGGATTTACAGCGG
CGCGGCGCAATGCACCTGCAGCGCGATTTGAAGGGCAGAATTGCGATGATGAATCGCCTGCAACAGGCGTTGGAGCACGA
TCATTTTTTCCTGATGGCGCAGCCTATTTTCGGTGTTCGTGGGGACGTTTACCATGAAATCTTACTGCGACTCAGGGATG
ACAACGGCGACGTTATCAACCCGGACAATTTTTTACCCGTGGCGCATGAGTTTGGATTGTCTTCCGCTATCGATCTTTGG
GTGATTGAAAACACGCTGCGGTTTATGGCGGCCAGCCGGGAATATATGCCCGCGCATCGTTTTGCCATTAATCTGTCGCC
AACATCGGTATGCCGCGCTCGTTTCCCTGCAGAAGTTAAGCAGCTGCTTACGCAATATCAGGTTGAACCCTGGCAACTGG
TTTTTGAGGTAACGGAAAGCAACTCTCTCACCAATGCTGAGCAGGCGCAGTTGACGCTGGGACAGCTACAGCAGTTTGGC
TGTCAGATAGCGATTGATGATTTCGGTACTGGCTACGCCAGCTATGCGCGGCTGAAAAACGTCGACGCCGATATTCTTAA
AATTGATGGCAGTTTTATTCGCAATATAGTGTCAAACAGTCTCGATTATCAGATTGTGGCTTCTATTTGTCATTTAGCGC
GAATGAAAAATATGCAGGTCGTCGCGGAGTATGTCGAGAGCGAAGAAATACGAAGCGTAGTGCTCTCGTTGGGGATCGAC
TATCTTCAGGGATATTTAATTGGCGAGCCGCAGCTATTATCTGAGATTCAGTAA

Upstream 100 bases:

>100_bases
ATGACTATCGTGTGATGTATATCTCATCATCAATTTAAAGTCAGGTGGGATGCCTGTCTGTTAACGCCCCTTTGGGATTG
GGGGGTTCTGCTGAGCATCT

Downstream 100 bases:

>100_bases
AGACAGACGCCGCGGCGCGGCGTCTGGCTCACAATTTACGCGGCGATCTCCGGCGAGCTTTCTTCCTCAATATTGAGACG
CCAGCCAGTTACCGCTTCCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRIFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Sequences:

>Translated_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRIFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ
>Mature_737_residues
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFFGWAALPGIVLAIFWRRYPQT
GLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHLLFQRIFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMP
FNINTLINYQALLVGNLVGVPLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSSSYLVFSFIVNYMSVLATRQR
VVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLHVPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQM
SGHDLVLRLNTEAHQQRIEALDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVINPDNFLPVAHEFGLSSAIDLW
VIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVKQLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFG
CQIAIDDFGTGYASYARLKNVDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYVESEEIRSVVLSLGID
YLQGYLIGEPQLLSEIQ

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=733, Percent_Identity=73.2605729877217, Blast_Score=1152, Evalue=0.0,
Organism=Escherichia coli, GI87082096, Length=741, Percent_Identity=28.4750337381916, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790496, Length=243, Percent_Identity=34.156378600823, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787541, Length=435, Percent_Identity=27.1264367816092, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI226510982, Length=417, Percent_Identity=26.6187050359712, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081743, Length=248, Percent_Identity=29.8387096774194, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786507, Length=231, Percent_Identity=31.6017316017316, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788502, Length=246, Percent_Identity=30.0813008130081, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI87081980, Length=249, Percent_Identity=29.3172690763052, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081921, Length=463, Percent_Identity=24.4060475161987, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI87081845, Length=234, Percent_Identity=28.6324786324786, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1787055, Length=251, Percent_Identity=27.0916334661355, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789650, Length=246, Percent_Identity=22.3577235772358, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 84272; Mature: 84272

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAALPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRIFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCC
VDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYVESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC
>Mature Secondary Structure
MKLNKTYINIRDKWWGLPLILPSILLPVLSSANTYALTSTGNVVLFYLPLAFMLSLMLFF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHH
GWAALPGIVLAIFWRRYPQTGLYETLSVTMHFIITIVLSWGGYRVFSPRRNNVSHGDAHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LFQRIFWQVFCSATLFLVIYQFAAFVGMYESKASLMGVMPFNINTLINYQALLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCHHHHHHHHHHHHHHHHHH
PLCYFIIRTLRNPLHLRGYYQQLKLQIDSKATKKEIVIWLAVLTTLMFILCMPLTDNSSI
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FSTNYTLSLLLPVMLWGAMRYGYKFISIIWAVVLITSIHYYQRYMPWYSGYDTQLAITSS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECC
SYLVFSFIVNYMSVLATRQRVVSGRARRLAYLDPVVHLPNLRALNRALQNAPWSTICFLH
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEE
VPGLELLGKNYGVMLRIQYKQKLSHWITPMLASNECVYQMSGHDLVLRLNTEAHQQRIEA
CCCHHHHCCCCCEEEEEEHHHHHHHHHCHHHCCCCEEEEECCCEEEEEECCHHHHHHHHH
LDKHIKQFRFIWDGLPLQPPVGVSYCCVRSPVSHLYLLLGELSTSSDLSLTTNAPEDLQR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEECCCCHHHHH
RGAMHLQRDLKGRIAMMNRLQQALEHDHFFLMAQPIFGVRGDVYHEILLRLRDDNGDVIN
HCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEC
PDNFLPVAHEFGLSSAIDLWVIENTLRFMAASREYMPAHRFAINLSPTSVCRARFPAEVK
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHH
QLLTQYQVEPWQLVFEVTESNSLTNAEQAQLTLGQLQQFGCQIAIDDFGTGYASYARLKN
HHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCC
VDADILKIDGSFIRNIVSNSLDYQIVASICHLARMKNMQVVAEYVESEEIRSVVLSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHH
YLQGYLIGEPQLLSEIQ
HHCCEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]