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The map label for this gene is pgtE

Identifier: 16765721

GI number: 16765721

Start: 2506055

End: 2506993

Strand: Reverse

Name: pgtE

Synonym: STM2395

Alternate gene names: 16765721

Gene position: 2506993-2506055 (Counterclockwise)

Preceding gene: 16765722

Following gene: 16765719

Centisome position: 51.61

GC content: 46.33

Gene sequence:

>939_bases
ATGAAAAAACATGCTATTGCAGTAATGATGATCGCCGTATTTTCTGAGTCGGTTTATGCGGAGTCTGCCTTATTTATTCC
GGACGTCTCTCCTGATAGCGTCACGACATCCCTTTCCGTGGGTGTGTTAAATGGTAAATCCAGGGAGCTGGTTTATGATA
CCGACACCGGGCGGAAGCTGAGTCAACTGGATTGGAAAATAAAAAATGTCGCCACGTTGCAGGGGGATTTATCATGGGAA
CCCTATTCGTTCATGACGCTGGACGCCCGCGGCTGGACGTCTTTGGCGTCGGGATCGGGTCATATGGTTGACCATGACTG
GATGAGCAGTGAGCAGCCAGGCTGGACCGATCGTTCAATTCATCCGGACACCAGCGTCAACTATGCTAATGAATACGATT
TGAACGTGAAAGGTTGGTTATTGCAGGGCGATAACTACAAGGCGGGCGTAACAGCGGGCTATCAGGAAACCCGTTTTAGC
TGGACGGCAAGAGGCGGGTCTTATATTTATGATAATGGTCGATATATTGGTAATTTTCCTCATGGCGTGCGCGGCATAGG
TTATAGCCAGCGTTTCGAAATGCCCTATATCGGGCTGGCGGGTGATTATCGTATTAATGACTTTGAGTGTAATGTACTGT
TTAAATACAGCGACTGGGTAAATGCGCATGATAATGACGAACACTACATGCGCAAACTTACCTTCCGTGAAAAAACGGAA
AATTCACGATATTATGGCGCTTCTATTGACGCCGGATATTATATTACCAGTAATGCAAAAATCTTTGCTGAGTTTGCTTA
CAGTAAATATGAAGAAGGTAAGGGCGGTACGCAAATCATAGATAAAACCAGCGGTGATACGGCGTATTTTGGTGGCGATG
CCGCAGGTATAGCTAATAATAACTATACGGTTACCGCGGGGTTGCAGTATCGCTTCTAG

Upstream 100 bases:

>100_bases
CAAGTTCTCTGGCGTAATAAATGTACTCTTGTCCGACGATTTGACAAGATGAAAACTTCATCACCTCTCCAGATTACATC
TGAATATGAGGACAAGAGAA

Downstream 100 bases:

>100_bases
ACCACATCGGGATGTCATCGGTCATAACCGGCCGATGACGACTTTTTGCTGAACGTATGGCATGTCCGGTGATATTGCAT
AGGGGCAATAAAAGCAACAT

Product: outer membrane protease

Products: Hydrolyzed protein [C]

Alternate protein names: E protein

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANNNYTVTAGLQYRF

Sequences:

>Translated_312_residues
MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANNNYTVTAGLQYRF
>Mature_312_residues
MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKLSQLDWKIKNVATLQGDLSWE
PYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSIHPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFS
WTARGGSYIYDNGRYIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANNNYTVTAGLQYRF

Specific function: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family

Homologues:

Organism=Escherichia coli, GI1786777, Length=320, Percent_Identity=46.5625, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGTE_SALTY (P06185)

Other databases:

- EMBL:   M13923
- EMBL:   AF239770
- EMBL:   AE006468
- PIR:   B28255
- RefSeq:   NP_461336.1
- ProteinModelPortal:   P06185
- SMR:   P06185
- MEROPS:   A26.004
- PRIDE:   P06185
- GeneID:   1253917
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2395
- NMPDR:   fig|99287.1.peg.2317
- HOGENOM:   HBG468061
- OMA:   NSASIEY
- ProtClustDB:   PRK10993
- BioCyc:   STYP99287:STM2395-MONOMER
- GO:   GO:0006508
- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079
- Gene3D:   G3DSA:2.40.128.90
- PIRSF:   PIRSF001522
- PRINTS:   PR00482

Pfam domain/function: PF01278 Omptin; SSF69917 OM_adhesin/protease_omptin

EC number: 3.4.21.87 [C]

Molecular weight: Translated: 35055; Mature: 35055

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00834 OMPTIN_1; PS00835 OMPTIN_2; PS00018 EF_HAND_1

Important sites: ACT_SITE 104-104 ACT_SITE 106-106 ACT_SITE 226-226 ACT_SITE 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKL
CCCHHHHHHEEHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCH
SQLDWKIKNVATLQGDLSWEPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSI
HHHCEEEEEEEEEECCCCCCCEEEEEEECCCCEEECCCCCCEEECCCCCCCCCCCCCCCC
HPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGRYIGNFP
CCCCCCCCCCEECCCEEEEEEECCCEEECEECCCCCCEEEEEECCCCEEEECCEEEECCC
HGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
CCCCCCCCCCCCCCCEEECCCCEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANN
CCEEEEEECCCCEEEECCCEEEEEEHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCC
NYTVTAGLQYRF
CEEEEEEEEECC
>Mature Secondary Structure
MKKHAIAVMMIAVFSESVYAESALFIPDVSPDSVTTSLSVGVLNGKSRELVYDTDTGRKL
CCCHHHHHHEEHHHHHHHHCCCEEEECCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCH
SQLDWKIKNVATLQGDLSWEPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPGWTDRSI
HHHCEEEEEEEEEECCCCCCCEEEEEEECCCCEEECCCCCCEEECCCCCCCCCCCCCCCC
HPDTSVNYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGRYIGNFP
CCCCCCCCCCEECCCEEEEEEECCCEEECEECCCCCCEEEEEECCCCEEEECCEEEECCC
HGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHYMRKLTFREKTE
CCCCCCCCCCCCCCCEEECCCCEEECEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGDTAYFGGDAAGIANN
CCEEEEEECCCCEEEECCCEEEEEEHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCCCC
NYTVTAGLQYRF
CEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: EDTA; Tosyl-L-Lys chloromethyl ketone [C]

Metal ions: NA

Kcat value (1/min): 40 [C]

Specific activity: 58.7

Km value (mM): 0.0036 {plasminogen}} 0.000058 {plasminogen}} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Arginine; Benzamidine; Co2+; Cu2+; Diisopropyl fluorophosphate; Fe2+; NaCl; Tosyl -L-phenyl alaninechloromethyl ketone; Zn2+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3023201; 10869088; 11677609; 2651422