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The map label for this gene is menE

Identifier: 16765632

GI number: 16765632

Start: 2411673

End: 2413040

Strand: Reverse

Name: menE

Synonym: STM2305

Alternate gene names: 16765632

Gene position: 2413040-2411673 (Counterclockwise)

Preceding gene: 16765633

Following gene: 16765631

Centisome position: 49.68

GC content: 59.94

Gene sequence:

>1368_bases
ATGACATTCACGGATTGGCCGTGGCGCCACTGGCGTCAGGTTCGCAGTCAGGCGCCCGCTCTGCGCCTGAACGATGAAGT
GCTAAGCTGGCGCGCCCTCTGTGAGCGTATTGATGTGCTGGCGGGCGGGTTTGCCGCGCAGGGCGTACGCGAGGGCGATG
GTGTCCTGCTGCGCGCCGGGAATCAGCCGCGCACTCTGTTGGCATGGCTTGCGCTGATGCAGTGCGGCGCGCGGGTTCTG
CCCGTCAACCCGCAGTTGCCCCAGACATTGCTGGAGGCGCTGGTGCCGAAGTTAACGCTGCGTTTTGCGCTGACACTGGA
AGGCGAAAATGCGTTTTCTGGATTAACCGCGTTGCAGATACAAAAGAGTACAGCGGCGTATGCTGTGGCCTGGCAACCGC
AGCGTCTGGTTTCCATGACGCTGACATCGGGTTCCACAGGATTGCCAAAAGCGGCTGTACATACGTGCCAGGCGCATCTT
GCCAGCGCGCAGGGGGTGCTTTCGCTAATGCCGTTTGGCCCTCAGGATGACTGGCTGCTATCGCTGCCGCTGTTTCATGT
ATCCGGGCAGGGTATTATGTGGCGTTGGCTCTTCGCCGGGGCGCGCATGACGGTACGCGATAAGCAACCGCTGGAGCAGA
TGCTGGCAGGGTGTACTCATGCTTCGCTGGTGCCGACGCAGCTGTGGCGGCTGCTGGCGAATCGGGCGGCGGTGACGCTG
AAAGCCGTTTTGCTCGGCGGCGCGGTGATTCCGGTTGAACTCACCGACCAGGCCAGCAAACAAGGGATTCGCTGCTGGTG
CGGGTATGGGCTCACGGAGTTCGCGTCTACGGTATGCGCCAAAGAGGCCGATGGTTCGGATGATGTTGGCGCGCCGCTTC
CCGGCAGAGAGATCAGAATCGTCGATAATGAAGTCTGGCTGCGCGCCGCCAGTATGGCGGAAGGTTACTGGCGTGATGGC
AAACTGATCCCGCTGGTTAATGACGAAGGCTGGTTTGCCACGCGCGATCGCGGCGAGCTGAACCACGGCAGGCTAACCAT
CGCCGGGCGGCTGGACAACCTGTTCTTTAGCGGCGGCGAAGGCATTCAGCCGGAGGAAGTCGAACGCGTTATTAATGCGC
ATCCGCTGGTGCAACAAGCCTTCGTCGTGCCGGTAGAAGATAAAGAGTTTGGTCATCGTCCCGTTGCGGTTGTGGAGTAT
GCATCGCAGGCCGGAGACGTGAACCTCGCTGAATGGGTTCGTGATAAGCTGGCGCGTTTTCAACAGCCGGTTCGCTGGTT
GACGATGCCGTCAGAACTGAAAAACGGAGGGATTAAAATATCTCGCCGTGCGCTCCAGCAGTGGGTGTGTGAGAACTGTA
AAAATTAA

Upstream 100 bases:

>100_bases
CTGGACTGGATACCTTGCATCTGATGCAGGCGCAACAGATTCGCCCCTGGCCCGGTAGCGCGTTGCCTTGTCTGAAGCGT
GAGGAGCTGGAACGACTGTT

Downstream 100 bases:

>100_bases
TGTTAGGTTAATATACAACCATTCCATCGCTATTGCCGTTTTGTTTATCCGTTGATGTGTAACGTGTTATGAACAATCAG
CGTGAAACGGGGGCGCTATG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDVLAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTMPSELKNGGIKISRRALQQWVCENCKN

Sequences:

>Translated_455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDVLAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTMPSELKNGGIKISRRALQQWVCENCKN
>Mature_454_residues
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDVLAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVLP
VNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTALQIQKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLA
SAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTLK
AVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDGK
LIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYA
SQAGDVNLAEWVRDKLARFQQPVRWLTMPSELKNGGIKISRRALQQWVCENCKN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA)

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily

Homologues:

Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=79.0645879732739, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=496, Percent_Identity=24.3951612903226, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1786801, Length=416, Percent_Identity=25.7211538461538, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788107, Length=520, Percent_Identity=23.8461538461538, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=431, Percent_Identity=25.2900232018561, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=449, Percent_Identity=26.5033407572383, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=22.5609756097561, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=354, Percent_Identity=25.9887005649718, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=363, Percent_Identity=26.9972451790634, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=511, Percent_Identity=23.679060665362, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENE_SALTY (P37418)

Other databases:

- EMBL:   AE006468
- EMBL:   U02281
- RefSeq:   NP_461247.1
- ProteinModelPortal:   P37418
- PRIDE:   P37418
- GeneID:   1253827
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2305
- NMPDR:   fig|99287.1.peg.2228
- HOGENOM:   HBG547964
- OMA:   ADCTHAS
- ProtClustDB:   PRK09029
- BioCyc:   STYP99287:STM2305-MONOMER
- BRENDA:   6.2.1.26
- HAMAP:   MF_00731
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- TIGRFAMs:   TIGR01923

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.26

Molecular weight: Translated: 50244; Mature: 50113

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDVLAGGFAAQGVREGDGVLLRAG
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTALQI
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEE
QKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
ECCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCEEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTMPSELKNGGIKISRRALQQWVCENCKN
HCCHHEECCCHHHCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDVLAGGFAAQGVREGDGVLLRAG
CCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTALQI
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEE
QKSTAAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
ECCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCEEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTMPSELKNGGIKISRRALQQWVCENCKN
HCCHHEECCCHHHCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837