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The map label for this gene is yfaW [H]

Identifier: 16765618

GI number: 16765618

Start: 2398214

End: 2399431

Strand: Reverse

Name: yfaW [H]

Synonym: STM2291

Alternate gene names: 16765618

Gene position: 2399431-2398214 (Counterclockwise)

Preceding gene: 16765619

Following gene: 16765617

Centisome position: 49.4

GC content: 53.28

Gene sequence:

>1218_bases
ATGGAGAATATTATGACTCTTCCTAAAATTAAACACGTTCGAGCCTGGTTTATTGGGGGCGCTACCGCAGAGAAAGGGGC
TGGCGGCGGGGATTATCACGACCAGGGAGGAAATCACTGGATTGATGACCATATCGCCACTCCCATGAGCAAATATCGCG
ACTATGAGCAGTCTCGTCAGTCTTTTGGGATCAACGTGTTAGGGACGCTGATTGTCGAAGTAGAGGCGGAAAATAGGCAG
ACGGGGTTCGCCGTTTCGACAGCAGGGGAGATGGGCTGTTTTATCGTAGAAAAACATCTTAACCGGTTTATTGAAGGCAA
ATGCGTAAGCGATATCAAACTTATTCACGATCAGATGCTCGGCGCGACCATGTACTACTCCGGTTCCGGCGGTCTGGTGA
TGAACACGATTTCCTGTGTGGATCTGGCGTTGTGGGATTTGTTTGGCAAGGTGGTTGGGCTGCCGGTTTATAAATTGCTG
GGCGGAGCGGTGCGCGATGAAATTCAGTTTTACGCCACCGGCGCACGCCCGGATCTGGCTAAAGAAATGGGCTTTATCGG
CGGTAAAATGCCGACTCACTGGGGACCGCACGATGGTGATGCGGGGATCCGTAAAGATGCCGCGATGGTGGCGGATATGC
GTGAAAAGTGCGGGCCGGATTTCTGGCTGATGCTCGACTGCTGGATGAGTCAGGATGTGAACTATGCGACTAAACTGGCT
CACGCCTGTGCGCCATTTAATCTCAAATGGATTGAAGAGTGTCTGCCGCCGCAGCAGTATGAAGGCTATCGCGAATTAAA
ACGCAACGCGCCTGCAGGGATGATGGTTACCAGCGGCGAACACCATGGTACGCTGCAATCCTTCCGCACTCTGGCAGAAA
CCGGCATTGATATTATGCAGCCGGATGTTGGCTGGTGCGGCGGATTAACCACGCTGGTTGAGATCGCCGCGCTTGCCAAA
TCGCGCGGGCAACTGGTGGTGCCGCACGGTTCGTCTGTCTATTCGCACCATGCAGTTATTACTTTCACTAATACCCCTTT
CAGCGAGTTCCTGATGACCAGCCCGGACTGCTCCACTCTGCGTCCGCAATTTGACCCGATTCTCCTGGATGAGCCGGTGC
CGGTGAATGGACGCATCCATAAATCAGTGCTGGATAAGCCGGGCTTCGGCGTCGAGCTTAACCGTGACTGTCACTTGAAA
CGCCCTTATAGCCACTAA

Upstream 100 bases:

>100_bases
GAGAATCTAGATTATTTGGCCGGTAAAGCTATTGCCTGTGCCAAAGATATTTCACGCCTGTTGGGATGGAAAAGTCCCTT
CGACTCACTCGCATCTTAAA

Downstream 100 bases:

>100_bases
TAACTCGTGCGTTGCCTTTGTACGGCAACGCCCTCATCCTGTTGAGGATATTGCTATGAGCATCACTTTACTTGACGGCG
TAGTGAAGAAAAACCGCGCA

Product: galactonate dehydratase

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENRQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH

Sequences:

>Translated_405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENRQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH
>Mature_405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENRQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=96.0099750623441, Blast_Score=813, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44707; Mature: 44707

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENRQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHH
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCHHCC
PDVGWCGGLTTLVEIAALAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENRQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHH
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCHHCC
PDVGWCGGLTTLVEIAALAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA