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The map label for this gene is dacD

Identifier: 16765392

GI number: 16765392

Start: 2136025

End: 2137197

Strand: Reverse

Name: dacD

Synonym: STM2062

Alternate gene names: 16765392

Gene position: 2137197-2136025 (Counterclockwise)

Preceding gene: 16765393

Following gene: 16765391

Centisome position: 44.0

GC content: 52.86

Gene sequence:

>1173_bases
ATGCTGTTGAAACGCCGTTTGTTTATTGCCGCTTCTTTATTCGCGATGCACCTTTCTCCTGCGCTTGCGGCCGATGCCGT
AAGTTTTGCTCCCCAACCGCCAGCCATTGATGCAGGCGCCTGGGTGTTGATGGATTACACTACGGGACAGGTTCTTACCG
CCGGTAATGAACACCAGCAACGTAATCCCGCCAGCCTTACCAAGCTAATGACGGGCTACGTCGTCGATCGCGCCATCGAT
AGTCATCGTATCAGCCCTGACGATATCGTCACAGTTGGGCGCGACGCCTGGGCGAAGGATAATCCGGTGTTTGTCGGCTC
CTCGTTGATGTTTTTAAAAGAAGGGGATCGCGTGTCGGTGCGTGATTTAAGCCGTGGCTTAATCGTGGATTCCGGTAACG
ATGCCTGTGTGGCGCTGGCGGATTATATCGCGGGCGGGCAGCCGCAGTTTGTGGCGATGATGAACAGCTATGTGAAAAAA
CTCAATTTACAGGATACCCATTTTGAAACCGTCCACGGTCTGGATGCGCCGGGACAACATAGCTCCGCGTATGACCTGGC
CGTACTCTCCCGGGCGATTATTCACGGCGAGCCTGAATTTTATCACATGTACAGCGAGAAAAGCCTGACCTGGAACGGCA
TCACGCAGCAGAACCGTAACGGCTTGTTATGGGATAAGACCATGCATATCGATGGGCTTAAAACCGGACACACATCGGGG
GCGGGATTTAATCTTATCGCATCGGCGGTGGACGGGCAGCGTCGACTTATCGCCGTTGTGATGGGGGCGAAAAGTTCGAA
AGGTCGTGAGGAACAGGCGCGAAAACTGTTGCAGTGGGGCCAGCAAAACTTTGCGACGGTGCAGATTTTGCACAGCGGTA
AAAAAGTGGGCAGCGAGCGAATCTGGTATGGAGACAAAGAAAAGATTGCGCTGGGTACGGAACAGGACTTCTGGATGGCG
CTGCCTAAAGCGGAAATTCCGCATATCAAAGCAAAATATGTGCTGGATAGAAAAGAGCTGGAGGCGCCAATCGCCGCTCA
TCAACAGGTAGGTGAAATAGAGCTCTACGATCGGGATAAACTGATCGCTCAGTGGCCGCTGGTCACTCTGGAATCGGTGG
GTAAAGGCGGTATGTTCTCTCGTCTGAGCGACTATTTTCAGCATAAAGCGTAA

Upstream 100 bases:

>100_bases
CAAGAAAATGTGCCTTGTGTCACATTTTCTTGCAATTTATCCATGTTTGTTTAACTGAACTTCCGTAAAAAGAACGGCAA
ATAGAGACCATCCTGAGGAC

Downstream 100 bases:

>100_bases
GCCGTTGTCACACACACCGTTCACCGTCATCTGAGGTGTCAAAAGGGCGGGATTGCGAGGTTGCTCACAAAATGATCACG
CTGTCCTTGTGACCGTAAAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGQQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA

Sequences:

>Translated_390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGQQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA
>Mature_390_residues
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKK
LNLQDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGQQNFATVQILHSGKKVGSERIWYGDKEKIALGTEQDFWMA
LPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDKLIAQWPLVTLESVGKGGMFSRLSDYFQHKA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=87.3711340206186, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1786851, Length=387, Percent_Identity=48.062015503876, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787062, Length=385, Percent_Identity=47.5324675324675, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87082054, Length=274, Percent_Identity=26.6423357664234, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACD_SALTY (P37604)

Other databases:

- EMBL:   L31538
- EMBL:   L31538
- EMBL:   AE006468
- EMBL:   L32188
- PIR:   D57143
- RefSeq:   NP_461007.1
- ProteinModelPortal:   P37604
- SMR:   P37604
- MEROPS:   S11.009
- GeneID:   1253583
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2062
- HOGENOM:   HBG728991
- OMA:   WGLQNFA
- ProtClustDB:   PRK11397
- BioCyc:   STYP99287:STM2062-MONOMER
- BRENDA:   3.4.16.4
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967
- Gene3D:   G3DSA:2.60.410.10
- PRINTS:   PR00725
- SMART:   SM00936

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11; SSF56601 PBP_transp_fold; SSF69189 Peniciliin-bd_prot-assoc

EC number: =3.4.16.4

Molecular weight: Translated: 43098; Mature: 43098

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: ACT_SITE 65-65 ACT_SITE 68-68 ACT_SITE 131-131 BINDING 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQ
CCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKKLNLQDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGQQNFATVQILHSGKKVGSER
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCC
IWYGDKEKIALGTEQDFWMALPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDK
EEECCCCEEEECCCCCCEEECCCCCCCCCCHHEEECHHHHCCCHHHHHCCCCEEEECCCC
LIAQWPLVTLESVGKGGMFSRLSDYFQHKA
EEECCCCHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLLKRRLFIAASLFAMHLSPALAADAVSFAPQPPAIDAGAWVLMDYTTGQVLTAGNEHQQ
CCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRISPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQPQFVAMMNSYVKKLNLQDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMHIDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGAKSSKGREEQARKLLQWGQQNFATVQILHSGKKVGSER
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCC
IWYGDKEKIALGTEQDFWMALPKAEIPHIKAKYVLDRKELEAPIAAHQQVGEIELYDRDK
EEECCCCEEEECCCCCCEEECCCCCCCCCCHHEEECHHHHCCCHHHHHCCCCEEEECCCC
LIAQWPLVTLESVGKGGMFSRLSDYFQHKA
EEECCCCHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291