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The map label for this gene is cbiH

Identifier: 16765357

GI number: 16765357

Start: 2105999

End: 2106724

Strand: Reverse

Name: cbiH

Synonym: STM2027

Alternate gene names: 16765357

Gene position: 2106724-2105999 (Counterclockwise)

Preceding gene: 16765358

Following gene: 16765356

Centisome position: 43.37

GC content: 56.89

Gene sequence:

>726_bases
ATGTTGAGCGTAATTGGTATTGGCCCCGGTTCGCAGGCAATGATGACGATGGAGGCGATTGAGGCGCTTCAGGCGGCGGA
AATCGTCGTCGGTTATAAAACCTACACCCACCTGGTGAAAGCCTTTACCGGCGATAAGCAGGTGATCAAAACCGGGATGT
GCAGAGAGATTGAACGCTGCCAGGCGGCGATTGAGCTGGCGCAGGCCGGGCATAACGTGGCGCTGATTAGCAGCGGCGAC
GCCGGTATTTACGGCATGGCGGGACTGGTGCTGGAGCTGGTGAGCAAGCAGAAGCTGGATGTAGAGGTTCGCCTTATTCC
GGGGATGACCGCCAGTATCGCAGCCGCCTCGCTGCTGGGTGCGCCGCTGATGCACGACTTCTGTCATATCAGCCTCAGCG
ACCTGCTGACCCCGTGGCCGGTAATTGAAAAACGTATTGTTGCCGCCGGAGAGGCAGACTTTGTTATCTGTTTTTACAAC
CCGCGCAGCCGCGGCCGCGAAGGGCATCTGGCGCGCGCGTTTGACCTGCTTGCCGCCAGTAAAAGCGCGCAAACGCCGGT
TGGCGTGGTGAAATCCGCCGGACGTAAGAAAGAGGAAAAATGGCTGACTACGCTCGGCGATATGGATTTTGAACCGGTAG
ATATGACCAGTCTGGTCATCGTTGGTAATAAAACGACCTATGTTCAGGATGGGCTGATGATAACGCCGCGAGGTTACACG
CTGTGA

Upstream 100 bases:

>100_bases
GCGTATCCGGCCCGGCAGCGTGGTTATTAAGCCAGGGACAATTGTTAGGCGAGACCCTGCGAGAACAGGGCGTCACTATT
ACTTTAGGAGTTGCACACTG

Downstream 100 bases:

>100_bases
ATGAGGGGGATGTGCTGGTGGTGGGCGGCACCAGCGATGCGCGCGCGTTATGCCGACAACTGGATGCGGCGAACGTCGCG
TACACCTTATCCGTGGCGAC

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L

Sequences:

>Translated_241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L
>Mature_241_residues
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERCQAAIELAQAGHNVALISSGD
AGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLGAPLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYN
PRSRGREGHLARAFDLLAASKSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
L

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIH_SALTY (Q05590)

Other databases:

- EMBL:   L12006
- EMBL:   AE006468
- RefSeq:   NP_460972.1
- ProteinModelPortal:   Q05590
- PRIDE:   Q05590
- GeneID:   1253548
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2027
- NMPDR:   fig|99287.1.peg.1952
- HOGENOM:   HBG305606
- OMA:   EATPEMA
- ProtClustDB:   PRK15478
- BioCyc:   MetaCyc:MONOMER-13222
- BioCyc:   STYP99287:STM2027-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01466

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: 2.1.1.131

Molecular weight: Translated: 25870; Mature: 25870

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERC
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLG
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHC
APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS
CHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEECCCCCC
L
C
>Mature Secondary Structure
MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERC
CEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLG
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHC
APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS
CHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHC
KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEECCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609